Title:
METHOD FOR NON-FREEZE LOW-TEMPERATURE PRESERVATION OF MAMMALIAN EMBRYO OR FERTILIZED EGG
Document Type and Number:
WIPO Patent Application WO/2014/030211
Kind Code:
A1
Abstract:
Disclosed is a novel means which enables the satisfactory preservation of a mammalian embryo and a mammalian fertilized egg in a non-frozen state for a longer period compared with conventional means and also enables the hatchability and the conception rate of the embryo after the preservation to be maintained at higher levels. A method for preserving a mammalian embryo or fertilized egg according to the present invention comprises: immersing the mammalian embryo or fertilized egg in a culture medium containing 20 to 80% (V/V) of serum and 10 to 100 mM of a Good's buffer; and then preserving the embryo or fertilized egg under non-freeze low-temperature conditions. A preservation solution for a mammalian embryo or fertilized egg according to the present invention is essentially composed of a culture medium containing 20 to 80% (V/V) of serum and 10 to 100 mM of a Good's buffer. The Good's buffer is preferably HEPES.
Inventors:
IDETA ATSUSHI (JP)
AOYAGI YOSHITO (JP)
AOYAGI YOSHITO (JP)
Application Number:
PCT/JP2012/071079
Publication Date:
February 27, 2014
Filing Date:
August 21, 2012
Export Citation:
Assignee:
NAT FEDERATION AGRIC COOP ASS (JP)
IDETA ATSUSHI (JP)
AOYAGI YOSHITO (JP)
IDETA ATSUSHI (JP)
AOYAGI YOSHITO (JP)
International Classes:
C12N5/073; C12N5/075
Foreign References:
| JPS6472742A | 1989-03-17 | |||
| JP2010248160A | 2010-11-04 | |||
| JP2002233356A | 2002-08-20 |
Other References:
YOSHIAKI ONO ET AL.: "Sampling of fertilized eggs of cattle by use of Eagle's minimum essential medium added trisaminomethane, and the conservation and the transplantation", ANIMAL-HUSBANDRY, vol. 40, no. 3, 1986, pages 65 - 66
TSUCHIYA,H. ET AL.: "Short-term preservation of mouse oocytes at 5 °C", EXP.ANIM., vol. 50, no. 5, 2001, pages 441 - 443
SHOICHIRO YAMAGUCHI ET AL.: "Mouse Sojitsuhai no Tanki Teion Hozon", THE JAPANESE SOCIETY ZOOTECHNICAL SCIENCE ANNUAL MEETING ABSTRACTS, 20 March 2002 (2002-03-20), pages 106, V28-22
MASSIP,A. ET AL.: "Calving outcome following transfer of embryos produced in vitro in different conditions", ANIM. REPROD.SCI., vol. 44, 1996, pages 1 - 10, XP000982421, DOI: doi:10.1016/0378-4320(95)01467-5
TSUCHIYA,H. ET AL.: "Short-term preservation of mouse oocytes at 5 °C", EXP.ANIM., vol. 50, no. 5, 2001, pages 441 - 443
SHOICHIRO YAMAGUCHI ET AL.: "Mouse Sojitsuhai no Tanki Teion Hozon", THE JAPANESE SOCIETY ZOOTECHNICAL SCIENCE ANNUAL MEETING ABSTRACTS, 20 March 2002 (2002-03-20), pages 106, V28-22
MASSIP,A. ET AL.: "Calving outcome following transfer of embryos produced in vitro in different conditions", ANIM. REPROD.SCI., vol. 44, 1996, pages 1 - 10, XP000982421, DOI: doi:10.1016/0378-4320(95)01467-5
Attorney, Agent or Firm:
TANIGAWA, Hidejiro (JP)
Mountain stream Eijiro (JP)
Mountain stream Eijiro (JP)
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