Title:
METHOD FOR OBTAINING GENE MODULE AND ROUTE OF RICE LEAF SHEATH PROTOPLAST TRANSFORMATION
Document Type and Number:
WIPO Patent Application WO/2021/073122
Kind Code:
A1
Abstract:
Provided is a method for obtaining a gene module and route of rice sheath protoplast transformation, in particular: optimized conditions for transforming rice leaf sheath protoplast using a PEG-mediated method are provided, the conditions comprising the most suitable concentration ratio of protoplast to plasmid used for transformation, a PEG solution and amount that are used, the standing and culturing conditions once a rice leaf sheath protoplast suspension is added to the PEG solution, the pH values of related solutions, etc. It is confirmed by comparative experiments that the method of the present invention increases the transformation efficiency to 80%, and enables intelligent transformation components to function in 80% of plant cells.
Inventors:
GU XIAOFENG (CN)
LIU QING (CN)
LIU QING (CN)
Application Number:
PCT/CN2020/093803
Publication Date:
April 22, 2021
Filing Date:
June 01, 2020
Export Citation:
Assignee:
BIOTECHNOLOGY RES INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C12N15/82; C12N5/04
Foreign References:
| CN110656126A | 2020-01-07 | |||
| CN103710377A | 2014-04-09 | |||
| CN107488675A | 2017-12-19 | |||
| CN110241069A | 2019-09-17 | |||
| CN109810937A | 2019-05-28 |
Other References:
WEI LIU, HAO LIU, SHUANGYU DONG, FENGWEI GU, ZHIQIANG CHEN, JIAFENG WANG, HUI WANG: "Construction of Rice Leaf Sheath Protoplast Transformation System and Transient Expression of Pik-H4 and AvrPik-H4 Proteins", SCIENTIA AGRICULTURA SINICA, vol. 50, no. 23, 1 January 2017 (2017-01-01), pages 4575 - 4584, XP055802458, DOI: 10.3864/j.issn.0578-1752.2017.23.010
SUN HE, LANG ZHIHONG, ZHU LI, HUANG DAFANG: "Optimized condition for protoplast isolation from maize, wheat and rice leaves", CHINESE JOURNAL OF BIOTECHNOLOGY FEBRUARY, vol. 29, no. 2, 1 December 2013 (2013-12-01), pages 224 - 234, XP055802459, DOI: 10.13345/j.cjb.2013.02.007
LI, WENBIN ET AL.: "Transgenic rice plants produced by peg mediated plasmid uptake into protoplasts", ACTA BOTANICA SINICA, vol. 37, no. 5, 31 May 1995 (1995-05-31), XP009527395, ISSN: 0577-7496
SUN HE, LANG ZHIHONG, ZHU LI, HUANG DAFANG: "Optimized condition for protoplast isolation from maize, wheat and rice leaves", CHINESE JOURNAL OF BIOTECHNOLOGY FEBRUARY, vol. 29, no. 2, 1 December 2013 (2013-12-01), pages 224 - 234, XP055802459, DOI: 10.13345/j.cjb.2013.02.007
LI, WENBIN ET AL.: "Transgenic rice plants produced by peg mediated plasmid uptake into protoplasts", ACTA BOTANICA SINICA, vol. 37, no. 5, 31 May 1995 (1995-05-31), XP009527395, ISSN: 0577-7496
Attorney, Agent or Firm:
HAIHONG JIACHENG INTELLECTUAL PROPERTY & PARTNERS (CN)
Download PDF:
Previous Patent: PLANAR TRANSFORMER AND ACTIVE CIRCUIT
Next Patent: HYDROGENATION CATALYST MODIFIED WITH SOLID PHASE MODIFIER AND USE THEREOF
Next Patent: HYDROGENATION CATALYST MODIFIED WITH SOLID PHASE MODIFIER AND USE THEREOF