Title:
METHOD FOR OBTAINING SEQUENCE FOR NON-CYCLIC ARTIFICIAL NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2024/101294
Kind Code:
A1
Abstract:
[Problem] To provide a method for obtaining a sequence for a nucleic acid including an artificial nucleic acid, more specifically a method for amplifying a sequence for a nucleic acid including a non-cyclic artificial nucleic acid to amplify the sequence. [Solution] Provided is a method for obtaining a sequence for a polynucleotide comprising a non-cyclic artificial nucleic acid, the method comprising: a step of hybridizing a polynucleotide comprising a non-cyclic artificial nucleic acid having DNA fragments respectively attached to both ends thereof to a DNA polynucleotide pool including random sequences; a step of chemically linking a primer region to each of the both ends of the DNA polynucleotide; a step of determining the sequence for the DNA polynucleotide; and a step of determining the sequence for the polynucleotide comprising the non-cyclic artificial nucleic acid on the basis of the sequence for the DNA polynucleotide.
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Inventors:
NEMOTO NAOTO (JP)
TAKIZAWA SAE (JP)
ASANUMA HIROYUKI (JP)
MURAYAMA KEIJI (JP)
TAKIZAWA SAE (JP)
ASANUMA HIROYUKI (JP)
MURAYAMA KEIJI (JP)
Application Number:
PCT/JP2023/039831
Publication Date:
May 16, 2024
Filing Date:
November 06, 2023
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION SAITAMA UNIV (JP)
NATIONAL UNIV CORPORATION TOKAI NATIONAL HIGHER EDUCATION AND RESEARCH SYSTEM (JP)
NATIONAL UNIV CORPORATION TOKAI NATIONAL HIGHER EDUCATION AND RESEARCH SYSTEM (JP)
International Classes:
C12Q1/6869; C12N15/11; C12Q1/6813; C12Q1/6851
Domestic Patent References:
| WO2013073602A1 | 2013-05-23 |
Foreign References:
| US20100081801A1 | 2010-04-01 | |||
| CN111500572A | 2020-08-07 | |||
| CN109295187A | 2019-02-01 |
Other References:
MURAYAMA KEIJI, ASANUMA HIROYUKI: "Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers", CHEMBIOCHEM, JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 22, no. 15, 3 August 2021 (2021-08-03), Hoboken, USA, pages 2507 - 2515, XP093135127, ISSN: 1439-4227, DOI: 10.1002/cbic.202100184
KEIJI MURAYAMA, YOSHIHIRO TANAKA, TAKASUKE TODA, HIROMU KASHIDA, HIROYUKI ASANUMA: "Highly Stable Duplex Formation by Artificial Nucleic Acids Acyclic Threoninol Nucleic Acid ( a TNA) and Serinol Nucleic Acid (SNA) with Acyclic Scaffolds", CHEMISTRY - A EUROPEAN JOURNAL, JOHN WILEY & SONS, INC, DE, vol. 19, no. 42, 11 October 2013 (2013-10-11), DE, pages 14151 - 14158, XP055568099, ISSN: 0947-6539, DOI: 10.1002/chem.201301578
KEIJI MURAYAMA, YOSHIHIRO TANAKA, TAKASUKE TODA, HIROMU KASHIDA, HIROYUKI ASANUMA: "Highly Stable Duplex Formation by Artificial Nucleic Acids Acyclic Threoninol Nucleic Acid ( a TNA) and Serinol Nucleic Acid (SNA) with Acyclic Scaffolds", CHEMISTRY - A EUROPEAN JOURNAL, JOHN WILEY & SONS, INC, DE, vol. 19, no. 42, 11 October 2013 (2013-10-11), DE, pages 14151 - 14158, XP055568099, ISSN: 0947-6539, DOI: 10.1002/chem.201301578
Attorney, Agent or Firm:
KAWAGUTI & PARTNERS (JP)
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