Title:
METHOD FOR OBTAINING TARGET DNA FRAGMENTS
Document Type and Number:
WIPO Patent Application WO/2018/147071
Kind Code:
A1
Abstract:
The present invention provides a method whereby n types of IIs restriction enzymes are used, first to m-th DNA fragments are linked so as to be arranged in ascending order, and a target DNA fragment is obtained. The method comprises: a step (i) in which an m number of DNA fragments are separated into a plurality of reaction systems; a step (ii) in which DNA fragments in each reaction system are linked order-specifically, using IIs restriction enzymes; a step (iii) in which the obtained linking DNA fragments are separated into at least one reaction system; a step (iv) in which the linking DNA fragments in each reaction system are linked order-specifically, using IIs restriction enzymes; and a step (v) in which steps (iii) and (iv) are repeated until the target DNA fragment is obtained.
Inventors:
YACHIE NOZOMU (JP)
ADACHI DAISUKE (JP)
SATO CHIKAKO (JP)
SUGAHARA JUNICHI (JP)
ADACHI DAISUKE (JP)
SATO CHIKAKO (JP)
SUGAHARA JUNICHI (JP)
Application Number:
PCT/JP2018/001958
Publication Date:
August 16, 2018
Filing Date:
January 23, 2018
Export Citation:
Assignee:
SPIBER INC (JP)
International Classes:
C12N15/09; C12N9/16
Domestic Patent References:
| WO2011154147A1 | 2011-12-15 |
Foreign References:
| JP2004041083A | 2004-02-12 | |||
| JP2016103986A | 2016-06-09 | |||
| JP2004141025A | 2004-05-20 |
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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