Title:
METHOD FOR PERFORMANCE ANALYSIS AND SCREENING OF SEPTICAEMIA PROTEIN THERAPEUTIC AGENTS
Document Type and Number:
WIPO Patent Application WO/2012/128577
Kind Code:
A2
Abstract:
The present invention relates to the construction of an assay system for analysing the performance of LRR (leucine rich repeat) proteins, that are constituted by the repeat combination of LRR modules, as septicaemia-inhibiting protein therapeutic agents, and for screening protein therapeutic agents that exhibit an outstanding inhibiting performance from a sequence variation library. More specifically, the present invention relates to: a performance analysis method or screening method for septicaemia protein therapeutic agents, comprising the step of adding purified MD-2 protein to an assay system using a cell line in which an LPS signal transmission receptor is expressed on the surface; a performance analysis method or screening method for septicaemia protein therapeutic agents, comprising the step of adding a purified foreign MD-2 protein to a THP-1 cell line; the cell line for performance analysis or for screening; and a kit for septicaemia therapeutic agent screening or performance analysis, comprising the cell line and purified foreign MD-2.
Inventors:
LEE SEUNG GOO (KR)
KIM HAK SUNG (KR)
KIM YU JUNG (KR)
LEE SANG CHUL (KR)
HAN JI EUN (KR)
KIM SU JIN (KR)
SOHN JUNG HOON (KR)
HA JAE SEOK (KR)
KIM HAK SUNG (KR)
KIM YU JUNG (KR)
LEE SANG CHUL (KR)
HAN JI EUN (KR)
KIM SU JIN (KR)
SOHN JUNG HOON (KR)
HA JAE SEOK (KR)
Application Number:
PCT/KR2012/002091
Publication Date:
September 27, 2012
Filing Date:
March 22, 2012
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
KOREA ADVANCED INST SCI & TECH (KR)
LEE SEUNG GOO (KR)
KIM HAK SUNG (KR)
KIM YU JUNG (KR)
LEE SANG CHUL (KR)
HAN JI EUN (KR)
KIM SU JIN (KR)
SOHN JUNG HOON (KR)
HA JAE SEOK (KR)
KOREA ADVANCED INST SCI & TECH (KR)
LEE SEUNG GOO (KR)
KIM HAK SUNG (KR)
KIM YU JUNG (KR)
LEE SANG CHUL (KR)
HAN JI EUN (KR)
KIM SU JIN (KR)
SOHN JUNG HOON (KR)
HA JAE SEOK (KR)
International Classes:
C12Q1/02; C12N15/63; G01N33/15; G01N33/53; G01N33/68; G16B15/20; G16B15/30
Foreign References:
| EP1657257A1 | 2006-05-17 |
Other References:
MATSUNAGA, N. ET AL.: 'TAK242(Reasatorvid), a small-molecule inhibitor of Toll-like receptor(TLR) 4 signaling, binds selectively to TLR4 an interferes with interactions between TLR4 and its adaptor molecules' MOLECULAR PHARMACOLOGY vol. 79, no. 1, January 2001, pages 34 - 41
JUNG, K. ET AL.: 'Toll-like receptor 4 decoy, TOY, attenates gram-negative bacterial sepsis' PLOS ONE vol. 4, no. ISS.10, 09 October 2009, page E7403
WEISS, T. ET AL.: 'Anti-inflammatory effects of moxifloxacin on activated human monocytic cell: inhibition of NF-kB and mitogen-activated protein kinase activation and of synthesis of proinflammatory cytokines' ANTIMICROBIAL AGENTS AND CHEMOTHERAPY vol. 48, no. 6, June 2004, pages 1974 - 1982
PUGIN, J ET AL.: 'Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock' BLOOD vol. 104, 24 August 2004, pages 4071 - 4079
JUNG, K. ET AL.: 'Toll-like receptor 4 decoy, TOY, attenates gram-negative bacterial sepsis' PLOS ONE vol. 4, no. ISS.10, 09 October 2009, page E7403
WEISS, T. ET AL.: 'Anti-inflammatory effects of moxifloxacin on activated human monocytic cell: inhibition of NF-kB and mitogen-activated protein kinase activation and of synthesis of proinflammatory cytokines' ANTIMICROBIAL AGENTS AND CHEMOTHERAPY vol. 48, no. 6, June 2004, pages 1974 - 1982
PUGIN, J ET AL.: 'Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock' BLOOD vol. 104, 24 August 2004, pages 4071 - 4079
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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