Title:
METHOD FOR PREDETERMINING THE BINDING OF A GIVEN COMPOUND TO A MEMBRANE RECEPTOR
Document Type and Number:
WIPO Patent Application WO/2011/018586
Kind Code:
A3
Abstract:
The invention relates to a method that makes it possible to predetermine if a compound to be tested preferably binds to a membrane receptor R1 or to a membrane receptor R2, said receptors being known to be expressed as a monomeric, homodimeric, or heterodimeric receptor on the surface of cells. Said method is implemented through the use of one or two FRET partner pairs.
Inventors:
TRINQUET ERIC (FR)
ZWIER JURRIAAN (FR)
MATHIS GERARD (FR)
PIN JEAN-PHILIPPE (FR)
DURROUX THIERRY (FR)
ZWIER JURRIAAN (FR)
MATHIS GERARD (FR)
PIN JEAN-PHILIPPE (FR)
DURROUX THIERRY (FR)
Application Number:
PCT/FR2010/051700
Publication Date:
April 07, 2011
Filing Date:
August 12, 2010
Export Citation:
Assignee:
CIS BIO INT (FR)
CENTRE NAT RECH SCIENT (FR)
TRINQUET ERIC (FR)
ZWIER JURRIAAN (FR)
MATHIS GERARD (FR)
PIN JEAN-PHILIPPE (FR)
DURROUX THIERRY (FR)
CENTRE NAT RECH SCIENT (FR)
TRINQUET ERIC (FR)
ZWIER JURRIAAN (FR)
MATHIS GERARD (FR)
PIN JEAN-PHILIPPE (FR)
DURROUX THIERRY (FR)
International Classes:
G01N33/542
Domestic Patent References:
| WO2007116069A1 | 2007-10-18 | |||
| WO2008001361A2 | 2008-01-03 |
Foreign References:
| FR2890174A1 | 2007-03-02 |
Other References:
MAUREL D ET AL: "Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 329, no. 2, 15 June 2004 (2004-06-15), pages 253 - 262, XP004509821, ISSN: 0003-2697
MAUREL D ET AL: "Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: Application to GPCR oligomerization", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 5, no. 6, 1 June 2008 (2008-06-01), pages 561 - 567, XP002556321, ISSN: 1548-7091, [retrieved on 20080518]
MILLIGAN G: "Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors", EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 21, no. 4, 1 March 2004 (2004-03-01), pages 397 - 405, XP002350686, ISSN: 0928-0987, DOI: DOI:10.1016/J.EJPS.2003.11.010
ILIEN B ET AL: "Fluorescence resonance energy transfer to probe human M1 muscarinic receptor structure and drug binding properties", JOURNAL OF NEUROCHEMISTRY, WILEY INTERSCIENCE, NEW YORK, NY, US, vol. 85, no. 3, 1 May 2003 (2003-05-01), pages 768 - 778, XP002265260, ISSN: 0022-3042
MAUREL D ET AL: "Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: Application to GPCR oligomerization", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 5, no. 6, 1 June 2008 (2008-06-01), pages 561 - 567, XP002556321, ISSN: 1548-7091, [retrieved on 20080518]
MILLIGAN G: "Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors", EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 21, no. 4, 1 March 2004 (2004-03-01), pages 397 - 405, XP002350686, ISSN: 0928-0987, DOI: DOI:10.1016/J.EJPS.2003.11.010
ILIEN B ET AL: "Fluorescence resonance energy transfer to probe human M1 muscarinic receptor structure and drug binding properties", JOURNAL OF NEUROCHEMISTRY, WILEY INTERSCIENCE, NEW YORK, NY, US, vol. 85, no. 3, 1 May 2003 (2003-05-01), pages 768 - 778, XP002265260, ISSN: 0022-3042
Attorney, Agent or Firm:
NEVANT, Marc et al. (158 rue de l'Université, Paris Cedex 07, FR)
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