Title:
METHOD FOR PREPARING CONSTANT-TEMPERATURE AMPLIFICATION MIXED ENZYME SYSTEM
Document Type and Number:
WIPO Patent Application WO/2023/093061
Kind Code:
A1
Abstract:
Provided in the present invention is a method for preparing a constant-temperature amplification mixed enzyme system. Specifically, disclosed in the present invention is a method comprising: removing the vast majority of glycerol from a prepared glycerol-containing RPA enzyme system by means of dialysis, and then concentrating the enzyme system containing trace glycerol by using an ultrafiltration method. Experiments show that the amplification performance of an RPA mixed enzyme system prepared by means of such method is significantly improved.
Inventors:
JIANG XIWEN (CN)
LIAN XIANLAN (CN)
XIE XIAOCHENG (CN)
LU XUELAN (CN)
XU HAOJIAN (CN)
LIAN XIANLAN (CN)
XIE XIAOCHENG (CN)
LU XUELAN (CN)
XU HAOJIAN (CN)
Application Number:
PCT/CN2022/103697
Publication Date:
June 01, 2023
Filing Date:
July 04, 2022
Export Citation:
Assignee:
DAAN GENE CO LTD (CN)
International Classes:
C12Q1/6844
Domestic Patent References:
| WO2002101004A2 | 2002-12-19 |
Foreign References:
| US20010041334A1 | 2001-11-15 | |||
| CN113981045A | 2022-01-28 |
Other References:
SHI YI, XU CHANGPING;YU BEIBEI;LU YIYU;MEI LINGLING: "Research Progress in Recombinase Polymerase Amplification（RPA）", BINGDU XUEBAO - CHINESE JOURNAL OF VIROLOGY, ZHONGGUO WEISHENGWU XUCHUI, BEIJING,, CN, vol. 36, no. 3, 28 April 2020 (2020-04-28), CN , pages 522 - 532, XP093068376, ISSN: 1000-8721, DOI: 10.13242/j.cnki.bingduxuebao.003708
MILENA EULER; YONGJIE WANG; OLIVER NENTWICH; OLAF PIEPENBURG; FRANK T. HUFERT; MANFRED WEIDMANN;: "Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus", JOURNAL OF CLINICAL VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 54, no. 4, 9 May 2012 (2012-05-09), NL , pages 308 - 312, XP028425700, ISSN: 1386-6532, DOI: 10.1016/j.jcv.2012.05.006
WANG YA-NAN, CHEN CHANG-GUO: "Advances in the research of recombinase polymerase amplification technology", MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY, BEIJING : RENMIN JUNYI CHUBANSHE, CN, vol. 46, no. 5, 28 May 2021 (2021-05-28), CN , pages 504 - 511, XP093068388, ISSN: 0577-7402, DOI: 10.11855/j.issn.0577-7402.2021.05.13
KUI SUN, XING ，XU WEI-WEI : "Advances in recombinase polymerase amplification", MILITARY MEDICAL SCIENCES, CN, vol. 39, no. 10, 25 October 2015 (2015-10-25), CN , pages 802 - 804, XP093068393, ISSN: 1674-9960
MILENA EULER; YONGJIE WANG; OLIVER NENTWICH; OLAF PIEPENBURG; FRANK T. HUFERT; MANFRED WEIDMANN;: "Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus", JOURNAL OF CLINICAL VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 54, no. 4, 9 May 2012 (2012-05-09), NL , pages 308 - 312, XP028425700, ISSN: 1386-6532, DOI: 10.1016/j.jcv.2012.05.006
WANG YA-NAN, CHEN CHANG-GUO: "Advances in the research of recombinase polymerase amplification technology", MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY, BEIJING : RENMIN JUNYI CHUBANSHE, CN, vol. 46, no. 5, 28 May 2021 (2021-05-28), CN , pages 504 - 511, XP093068388, ISSN: 0577-7402, DOI: 10.11855/j.issn.0577-7402.2021.05.13
KUI SUN, XING ，XU WEI-WEI : "Advances in recombinase polymerase amplification", MILITARY MEDICAL SCIENCES, CN, vol. 39, no. 10, 25 October 2015 (2015-10-25), CN , pages 802 - 804, XP093068393, ISSN: 1674-9960
Attorney, Agent or Firm:
SHANGHAI GOOIP CO., LTD. (CN)
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