Title:
METHOD FOR PREPARING ERGOTHIONEINE BY BIOSYNTHESIS AND FERMENTATION MEDIUM
Document Type and Number:
WIPO Patent Application WO/2020/134687
Kind Code:
A1
Abstract:
A method for preparing ergothioneine by biosynthesis. The method comprises the following steps: 1) strain inoculating, on slant, mycelia of Hericium erinaceus into a liquid seed medium for cultivation, so as to obtain a seed liquid; 2) inoculating the obtained seed liquid into a fermentation medium for fermentation, adding a precursor substance for cultivation, and determining the fermentation progress by means of pH till the endpoint of the fermentation, so as to obtain a fermentation broth; and (3) adding enzymes after the fermentation is completed, so as to perform enzymolysis till the endpoint, and performing heating to inactivate the enzymes, so as to leach the ergothioneine from the cells of the mycelia into the fermentation broth outside the cells. Further provided is a method for determining an endpoint of fermentation of ergothioneine and a fermentation medium.
Inventors:
WEI YUJIE (CN)
LU ZHEN (CN)
JIA YUQIAN (CN)
SUN YUANJUN (CN)
CHEN WENWEN (CN)
SHI YANLI (CN)
LUAN YIHONG (CN)
GUO XUEPING (CN)
LU ZHEN (CN)
JIA YUQIAN (CN)
SUN YUANJUN (CN)
CHEN WENWEN (CN)
SHI YANLI (CN)
LUAN YIHONG (CN)
GUO XUEPING (CN)
Application Number:
PCT/CN2019/118946
Publication Date:
July 02, 2020
Filing Date:
November 15, 2019
Export Citation:
Assignee:
BLOOMAGE BIOTECHNOLOGY CORP LTD (CN)
SHANDONG BLOOMAGE HYINC BIOPHARM CORPORATION LTD (CN)
SHANDONG BLOOMAGE HYINC BIOPHARM CORPORATION LTD (CN)
International Classes:
C12P13/04; C12R1/645
Foreign References:
| CN109439701A | 2019-03-08 | |||
| CN105296559A | 2016-02-03 |
Other References:
MEI, BAOLIANG ET AL.: "Study on the Biosynthesis of L-ergothioneine by Enhancement of Nutritional Factors", FOOD RESEARCH AND DEVELOPMENT, vol. 36, no. 15, 31 August 2015 (2015-08-31), DOI: 20191220090216Y
KALARAS, M. D. ET AL.: "Mushrooms: A rich source of the antioxidants ergothioneine and glutathione", FOOD CHEMISTRY, 20 April 2017 (2017-04-20), XP085491844, DOI: 20191220090710Y
JIANG, LI ET AL.: "A Preliminary Study on Submerged Fermentation Process of Hericium Erinaceus", FOOD SCIENCE, vol. 22, no. 2, 31 December 2001 (2001-12-31), DOI: 20191220091122Y
JIANG, LI ET AL.: "A Preliminary Study on Submerged Fermentation Process of Hericium Erinaceus", FOOD SCIENCE, vol. 22, no. 2, 31 December 2001 (2001-12-31), DOI: 20191220134523X
LIU, MEISEN ET AL.: "Non-official translation: Research Progress on Hericium Erinaceus Liquid Fermentation", FOOD SCIENCE, vol. 19, no. 6, 31 December 1998 (1998-12-31), DOI: 20191220091312Y
LIU, MEISEN ET AL.: "Non-official translation: Research Progress on Hericium Erinaceus Liquid Fermentation", FOOD SCIENCE, vol. 19, no. 6, 31 December 1998 (1998-12-31), DOI: 20191220134539X
KALARAS, M. D. ET AL.: "Mushrooms: A rich source of the antioxidants ergothioneine and glutathione", FOOD CHEMISTRY, 20 April 2017 (2017-04-20), XP085491844, DOI: 20191220090710Y
JIANG, LI ET AL.: "A Preliminary Study on Submerged Fermentation Process of Hericium Erinaceus", FOOD SCIENCE, vol. 22, no. 2, 31 December 2001 (2001-12-31), DOI: 20191220091122Y
JIANG, LI ET AL.: "A Preliminary Study on Submerged Fermentation Process of Hericium Erinaceus", FOOD SCIENCE, vol. 22, no. 2, 31 December 2001 (2001-12-31), DOI: 20191220134523X
LIU, MEISEN ET AL.: "Non-official translation: Research Progress on Hericium Erinaceus Liquid Fermentation", FOOD SCIENCE, vol. 19, no. 6, 31 December 1998 (1998-12-31), DOI: 20191220091312Y
LIU, MEISEN ET AL.: "Non-official translation: Research Progress on Hericium Erinaceus Liquid Fermentation", FOOD SCIENCE, vol. 19, no. 6, 31 December 1998 (1998-12-31), DOI: 20191220134539X
Attorney, Agent or Firm:
ZHONGZI LAW OFFICE (CN)
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