Title:
METHOD FOR PREPARING INFLUENZA HEMAGGLUTININ GLYCOPROTEIN HAVING ANIMAL-CELL SUGAR-BASED MODIFICATION BY USING SUGAR-BASED ENGINEERING YEAST
Document Type and Number:
WIPO Patent Application WO/2016/078466
Kind Code:
A1
Abstract:
The present invention provides a method for preparing an influenza virus hemagglutinin glycoprotein having a mammal sugar-type structure and no fucose. The method comprises: making influenza virus hemagglutinin HA gene express in a yeast mutant, to obtain a recombinant yeast; and culturing the recombinant yeast, to obtain the influenza virus hemagglutinin glycoprotein having a mammal sugar-type structure and no fucose.
Inventors:
LIU BO (CN)
WU JUN (CN)
GONG XIN (CN)
CHANG SHAOHONG (CN)
WANG SHA (CN)
MA QINGJUN (CN)
WU JUN (CN)
GONG XIN (CN)
CHANG SHAOHONG (CN)
WANG SHA (CN)
MA QINGJUN (CN)
Application Number:
PCT/CN2015/088745
Publication Date:
May 26, 2016
Filing Date:
September 01, 2015
Export Citation:
Assignee:
INST OF BIOTECHNOLOGY ACADEMY OF MILITARY MEDICAL SCIENCES CHINA (CN)
International Classes:
C12P21/00; C07K14/11; C12N1/19; C12R1/645; C12R1/78; C12R1/84
Foreign References:
| CN102459603A | 2012-05-16 |
Other References:
SUBATHRA, M. ET AL.: "Recombinant Haemagglutinin Protein of Highly Pathogenic Avian Influenza A (H5N1) Virus Expressed in Pichia Pastoris Elicits a Neutralizing Antibody Response in Mice", JOURNAL OF VIROLOGICAL METHODS, vol. 187, no. 2, 31 July 2013 (2013-07-31), pages 20 - 25, XP055180947, DOI: doi:10.1016/j.jviromet.2012.07.026
TSAI, S.M. ET AL.: "Novel Post-translational Modifications of the Hemagglutinin and Neuraminidase Proteins of Avian Influenza Virus Expressed by Kluyveromyces Lactis", JOURNAL OF VIROLOGICAL METHODS, vol. 175, no. 1, 26 September 2015 (2015-09-26), pages 175 - 181
TSAI, S.M. ET AL.: "Novel Post-translational Modifications of the Hemagglutinin and Neuraminidase Proteins of Avian Influenza Virus Expressed by Kluyveromyces Lactis", JOURNAL OF VIROLOGICAL METHODS, vol. 175, no. 1, 26 September 2015 (2015-09-26), pages 175 - 181
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
北京纪凯知识产权代理有限公司 (CN)
北京纪凯知识产权代理有限公司 (CN)
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