Title:
METHOD FOR PREPARING LACTIC ACID BACTERIUM-DERIVED EXTRACELLULAR VESICLES WITH IMPROVED YIELD
Document Type and Number:
WIPO Patent Application WO/2023/177208
Kind Code:
A1
Abstract:
The present invention relates to a method for preparing lactic acid bacterium-derived extracellular vesicles with improved yield. The inventors of the present invention have identified the optimal medium composition and fermentation conditions that can increase the yield of extracellular vesicles secreted by lactic acid bacteria. Specifically, there were problems with the conventional Lactobacillus culture medium, such as a small yield of extracellular vesicle, or inedible components of the medium, and toxicity due to animal-derived components. However, the present inventors have developed a medium that is not toxic, contains no animal-derived components, and is edible, and confirming that the medium is free of cytotoxicity and increases the yield of extracellular vesicles when culturing Lactobacillus. Furthermore, the present inventors identified the optimal culture time that can increase the yield. Therefore, the method of preparing extracellular vesicles with improved yield according to the present invention is expected to be beneficially used in processes for mass production on an industrial scale of extracellular vesicles secreted by lactic acid bacteria, which can be utilized as pharmacological components.
Inventors:
KIM YOON-KEUN (KR)
SHIN TAE SEOP (KR)
SHIN TAE SEOP (KR)
Application Number:
PCT/KR2023/003468
Publication Date:
September 21, 2023
Filing Date:
March 15, 2023
Export Citation:
Assignee:
MD HEALTHCARE INC (KR)
International Classes:
C12N1/20; C12N1/02; C12R1/01; C12R1/225; C12R1/46
Domestic Patent References:
| WO2008148561A1 | 2008-12-11 |
Foreign References:
| KR20220033010A | 2022-03-15 | |||
| KR101997468B1 | 2019-07-08 | |||
| KR102269964B1 | 2021-06-28 | |||
| EP3705566A1 | 2020-09-09 |
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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