Title:
METHOD FOR PREPARING LONG-CHAIN RNA MODIFIED AT SPECIFIC SITE
Document Type and Number:
WIPO Patent Application WO/2022/170705
Kind Code:
A1
Abstract:
Provided is a method for preparing long-chain RNA, the method comprising a synthesis step, an annealing step, and a linking step. In the method for preparing long-chain RNA, an RNA segment of a first chain and a nucleic acid segment in a second chain are designed, so that chemical modification for any precise sites in long-chain RNA can be realized, so that the long-chain RNA obtains improved stability, improved immunogenicity, etc. By means of the preparation method, a double-stranded assembly formed by means of the complementation of continuous single-stranded RNA and fragmented single-stranded nucleic acid chains can be obtained, and single-stranded long-chain RNA can be obtained from the double-stranded assembly merely by means of simple denaturation, so that the current synthesis step of the long-chain RNA is effectively simplified, the synthesis efficiency is improved, and the preparation method is suitable for industrial large-scale preparation.
Inventors:
LIU DONGSHENG (CN)
Application Number:
PCT/CN2021/098422
Publication Date:
August 18, 2022
Filing Date:
June 04, 2021
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C12N15/10
Foreign References:
| CN103993002A | 2014-08-20 | |||
| CN1898397A | 2007-01-17 | |||
| CN103865922A | 2014-06-18 | |||
| CN1131157A | 1996-09-18 | |||
| CN106536734A | 2017-03-22 |
Other References:
MARKO NICHOLAS F; FRANK BRYAN; QUACKENBUSH JOHN; LEE NORMAN H: "A robust method for the amplification of RNA in the sense orientation", BMC GENOMICS, vol. 6, no. 1, 1 March 2005 (2005-03-01), London, UK , pages 27, XP021002289, ISSN: 1471-2164, DOI: 10.1186/1471-2164-6-27
A. H. EL-SAGHEER, T. BROWN: "New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 35, 31 August 2010 (2010-08-31), pages 15329 - 15334, XP055084941, ISSN: 00278424, DOI: 10.1073/pnas.1006447107
FRANCINE E. WINCOTT: "Strategies for Oligoribonucleotide Synthesis According to the Phosphoramidite Method", CURRENT PROTOCOLS IN NUCLEIC ACID CHEMISTRY, JOHN WILEY & SONS, INC., US, vol. Unit 3.5, 31 May 2001 (2001-05-31), US , pages 3.5.1 - 3.5.12, XP009538842, ISSN: 1934-9289, DOI: 10.1002/0471142700.nc0305s00
A. H. EL-SAGHEER, T. BROWN: "New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 35, 31 August 2010 (2010-08-31), pages 15329 - 15334, XP055084941, ISSN: 00278424, DOI: 10.1073/pnas.1006447107
FRANCINE E. WINCOTT: "Strategies for Oligoribonucleotide Synthesis According to the Phosphoramidite Method", CURRENT PROTOCOLS IN NUCLEIC ACID CHEMISTRY, JOHN WILEY & SONS, INC., US, vol. Unit 3.5, 31 May 2001 (2001-05-31), US , pages 3.5.1 - 3.5.12, XP009538842, ISSN: 1934-9289, DOI: 10.1002/0471142700.nc0305s00
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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