Title:
METHOD FOR PREPARING OLIGONUCLEOTIDE BY USING RNA LIGASE
Document Type and Number:
WIPO Patent Application WO/2024/051143
Kind Code:
A1
Abstract:
Provided is a method for preparing an oligonucleotide by using an RNA ligase. The RNA ligase includes any one or more enzymes of the RNA ligase families Rnl1, Rnl2, Rnl3, and Rnl5, and the oligonucleotide includes natural RNA or non-natural RNA. The method can solve the problem that in the prior art, it is difficult to efficiently synthesize non-natural RNA chains, and the method is suitable for the field of RNA synthesis.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
WANG ZHAOSHUAI (CN)
LIU FANG (CN)
MA CUIPING (CN)
GENG YUHAN (CN)
YANG YIMING (CN)
LI JUAN (CN)
CUI LIXIN (CN)
ZHU WENXUAN (CN)
JIA XU (CN)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
WANG ZHAOSHUAI (CN)
LIU FANG (CN)
MA CUIPING (CN)
GENG YUHAN (CN)
YANG YIMING (CN)
LI JUAN (CN)
CUI LIXIN (CN)
ZHU WENXUAN (CN)
JIA XU (CN)
Application Number:
PCT/CN2023/082298
Publication Date:
March 14, 2024
Filing Date:
March 17, 2023
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N15/11; C12P19/34
Domestic Patent References:
| WO2021108647A1 | 2021-06-03 |
Foreign References:
| CN114430776A | 2022-05-03 | |||
| CN113439085A | 2021-09-24 | |||
| CN111479923A | 2020-07-31 | |||
| CN111819280A | 2020-10-23 | |||
| US20100279305A1 | 2010-11-04 |
Other References:
TURUNEN, J. ET AL.: "eBook available inter alia online, c.f. chapter 8.3.3 ", 22 June 2015, WILEY-VCH VERLAG GMBH & CO. KGAA, Weinheim, Germany , ISBN: 978-3-527-32776-8, article JANNE J. TURUNEN, LIUDMILA V. PAVLOVA, MARTIN HENGESBACH, MARK HELM, SABINE MÜLLER, ROLAND K. HARTMANN, MIKKO J. FRILANDER: "Chapter 3: Connection of RNA", pages: 45 - 88, XP009554077, DOI: 10.1002/9783527647064.ch3
Attorney, Agent or Firm:
KANGXIN PARTNERS , P.C. (CN)
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