Title:
METHOD FOR PREPARING PHOSPHATIDYLSERINE BY ULTRASONIC ASSISTED ENZYMOLYSIS
Document Type and Number:
WIPO Patent Application WO/2020/133126
Kind Code:
A1
Abstract:
Disclosed is a method for preparing phosphatidylserine by ultrasonic assisted enzymolysis, comprising the following steps: adding 100-130 parts of a phospholipid to a mixture containing 150-200 parts of L-serine, 10-20 parts of anhydrous calcium chloride, and 400-500 parts of pure water; adding 20-25 parts of phospholipase D to carry out an enzymolysis reaction; and applying an ultrasonic treatment during the enzymolysis reaction. The ultrasonic treatment technology is employed to assist phospholipase D in the enzymolysis reaction of phosphatidylcholine and serine for preparing phosphatidylserine. Parameters of the ultrasonic treatment, such as ultrasonic frequency, ultrasonic intensity, ultrasonic power, ultrasonic time, ultrasonic temperature and enzyme activity, are coordinately controlled, such that the enzymolysis conversion rate reaches 98% and above.
Inventors:
SUN YI (CN)
XIA HONGZHI (CN)
LI JIANGBO (CN)
TANG BO (CN)
JIN FENG (CN)
ZHU YULEI (CN)
XIA HONGZHI (CN)
LI JIANGBO (CN)
TANG BO (CN)
JIN FENG (CN)
ZHU YULEI (CN)
Application Number:
PCT/CN2018/124581
Publication Date:
July 02, 2020
Filing Date:
December 28, 2018
Export Citation:
Assignee:
NANTONG LICHENG BIOLOGICAL ENG CO LTD (CN)
International Classes:
C12P13/06
Domestic Patent References:
| WO2002012532A1 | 2002-02-14 |
Foreign References:
| CN107142289A | 2017-09-08 | |||
| CN103555783A | 2014-02-05 | |||
| CN107034248A | 2017-08-11 |
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM (ORDINARY PARTNERSHIP) (CN)
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