[Specification]

A METHOD FOR PREPARING PIG PLACENTAL EXTRACT TO USE AS A FEED ADDITIVE AND USE THEREOF [Technical Field]

The present invention relates to a method for preparing the pig placental extract for the feed additive and its use as a animal feed, more specifically, to the method for preparing the pig placental extract in which the pig placenta which is discharged from a farmhouse is treated by hot water extract, acidic or alkaline hydrolysis or pulverization to obtain crude protein for the purpose of using the pig placenta as the feed additive capable of producing quality meat. [Background Art]

The placenta, which is an organ connecting a fetus and the mother's womb, is known to have a diversity of function for the growth of the fetus that it can supply oxygen and various kinds of nutrients necessary for growth from the mother's body to a fetus as well as producing a hormone necessary to a fetus and synthesizing a factor necessary for enhancing immunity.

The placenta is discharged from the mother's body when the fetus is born. The placenta contains a plenty of effective ingredient such as various kinds of amino acid, active peptide, vitamin, mineral, enzyme and a variety of the growth factor. Owing to the effective ingredients, the placenta has been called as "Jahageo" in "Bonchogangmok" and "Dongeuybogam" from ancient times, and has been used in the traditional oriental medicines as well as its efficacy has attained attention for the purpose of maintaining beauty and health, recently. In addition, there is an opinion in a traditional oriental medicine that if a patient eats a part of animal corresponding to the

diseased part, he can recover, but it can be considered that it is efficacious to provide effective ingredients lacking in the diseased part.

From ancient times, by making an estimate of such a potential function, the placenta has been used as a medicine, however components and a medicinal efficacy are not clarified in detailed. However, by the diversity of study in biochemistry, it has been gradually discovered that the placenta has a various function such as anti-oxidation effect, anti-inflammation effect, injury remedial effect, cosmetic effect, fatigue recovery, immunity increment, habitude improvement and hematogenous functions.

Amino acid contained in the placenta extract, which is the source of life, have a diversity of physiological function as a factor in forming biological tissues and controlling in vivo chemical reaction. Among them, mineral functions in an intracellular metabolism and functions in controlling a blood and a body fluid, and a fatty acid functions as an important factor in forming a cell.

Meanwhile, the feed for a domestic animal contains a proper amount of protein, vitamin, mineral and fatty acid et al., in which protein did not digested and absorbed fully as amino acid and has a tendency to discard with feces. In such reasons, if the feed intake of the domestic animals is reduced due to the excessive stress such as the warmth, the disease or the dense stall, the immunity function of domestic animals is depreciated and it bring about indisposition of the disease defense system to cause the stagnation in growth. In the mean time, it is difficult to add artificially higher saturated and unsaturated fatty acids and organic chelating mineral constituents with the large amount because it is expensive in the market.

Accordingly, the inventors contrive the present invention in the decision that it can be possible to stimulate various kinds of metabolism of domestic animals and to

reduce an outbreak of disease due to a diversity of stress and thus to increase growth capability and productivity of domestic animals with increasing contents of amino acid, mineral and fatty acid susceptible to lacking in the existing animal feed, by the method of recovering the pig placenta which is higher nutritive resources but discarded from the farmhouse presently, processing and adding into the animal feed, and also to contribute to decrease an environmental contamination to some degree with reducing the pig placenta waste of the farmhouse. [Disclosure] [Technical Problem]

The object of the present invention is to provide the pig placental extract capable of using as a feed additive to produce quality meat and the preparation method thereof.

Another object of the present invention is to clarify the use of the pig placental extract as a feed additive by adding the pig placental extract to an animal feed and observing the growth of animal. [Technical Solution]

The above object is solved by preparing the pig placental extract via the method of hot water extract, acidic or alkaline hydrolysis, or pulverization, examining the obtained pig placental extract by each method and adding the pig placental extract into an animal feed and then observing the growth of animal.

According to the one aspect of the present invention, the method for preparing the pig placental extract comprises: mixing 1 kg of pig placental with 500 mL of 3N HCl or 500 mL of 3N NaOH and acidic or alkaline hydrolyzing the mixture or extracting the mixture with 500 mL of

hot water at 110 ^° C for 5 to 12 hours; removing a solid by filtering the hydrolyzed solution or hot water extract to obtained the pig placental extract; concentrating the pig placental extract without a solid and neutralizing the concentrated extract by treating with an anion exchange resin to neutralize; filtering the neutralized pig placental extract and sterilizing at 121 ^° C for 15 minutes.

The above acidic or alkaline hydrolysis is conducted for 5 to 12 hours, preferably 5 hours.

The pig placental extract prepared by the present invention can be used as an animal drinking water additive.

The content of nitrogen in the pig placental extract for using as an animal drinking water additive is suitably 0.1 to 0.4 %, preferably 0.4 %.

According to the one example of the present invention, the pig placental extract for animal feed additive is prepared by the acidic hydrolyzing the pig placental at 110 ^° C for 5 hours and alkaline hydrolyzing the hydrolyzed solution of the pig placental of pH 2 and the pig placental with NaOH, mixing one of the hydrolyzed solution of the pig placental of pH 10, a hot water extract of the placenta or a powder of the placenta with excipient in the ratio of 1:1, and drying the mixture to the water content of less than 10 %.

In case of feeding the animal feed containing 0.2 to 0.4 wt% of the pig placental extract for the animal feed additive to animal, there is an effect of promoting the growth of animal.

The content of nitrogen in the pig placental extract for using as an animal feed

additive is suitably 0.1 to 0.4 %, preferably 0.4 %.

The present invention will be explained with reference to the examples described hereinafter. However, these examples merely illustrates the present invention, they do not restricted the invention. [Effect of the invention]

The present invention which relates to the method for preparing the pig placental extract and its use as a animal feed, has an effect on providing the method for preparing the pig placental extract for the animal drinking water additive or feed additive. The pig placental extract of the present invention used as the animal drinking water additive or feed additive to effect on producing quality meat by the promotion of the growth of animal. [Brief description of the figures]

Figure 1 is a schematic diagram showing a manufacturing process of the animal drinking water additive or feed additive using the pig placental by the acidic hydrolysis method.

Figure 2 is a schematic diagram showing a manufacturing process of the animal feed additive by the alkaline hydrolysis method.

Figure 3 are graphs showing a Daily gain, A and Feed conversion rate, B of ICR mouse grown by feeding a drinking water containing the pig placental extract obtained by the acidic hydrolysis method for 9 days.

Figure 4 are graphs showing a change of weight and daily weight gain of pig grown by feeding a feed containing the pig placental extract obtained by the acidic hydrolysis method for 19 days.

Figure 5 is a graph showing an effect on daily weight gain and feed conversion

rate of mouse grown by feeding a feed containing the pig placental feed additive or the pig placental powder obtained by hot water extract of alkaline hydrolysis method.

Figure 6 is a graph showing an effect of the pig placental feed additive or the pig placental powder obtained by hot water extract of alkaline hydrolysis method on daily weight gain of weaning pig. [Best Mode for Carrying Out the Invention]

Example 1: Preparation of the pig placental extract

The frozen pig placental was obtained from the Hyoung-jae farm(Kimcheon, Kyengbuk, Korea) and the Hanareum farm(Yongin, Kyeongki, Korea), and the pig placental was thawed and washed to remove the umbilical cord blood and the amnion.

Preparation example 1: Preparation of the pig placental extract by the acidic hydrolysis method

1 kg of the washed pig placental was mixed with 500 mL of 3N HCl and the mixture was hydrolyzed at 110 ^° C for about 5 hours, and the next process was continued after confirming complete hydrolysis of protein by the Burette reaction and the trichroloacetic acid reaction. After confirming the negative response of protein, a solid was removed by filtering with 8 μm filter. The pig placental extract without a solid was condensed and neutralized by treating with anion exchange resin (pH 7). The neutralized pig placental extract was filtered with 0.45 and 0.2 μm filters to remove the residue and sterilized at 121 ^° C for 15 minutes to obtain the drinking water preparation containing the pig placental extract. The content of the crude protein was measured by the Kildal method. And then, it was used for the test of feeding the drinking water.

1 kg of the washed pig placental was mixed with 500 mL of 3N HCl and the

mixture was hydrolyzed at 110 ^° C for about 5 hours and then the hydrolyzed solution of the pig placental was treated with NaOH to adjust to pH 2. The hydrolyzed solution of the pig placental of pH 2 was mix with corncob in the ratio of 1 : 1 and the mixture was dried to the water content of 10%, and then it was used as the feed additive. The content of the feed additive are showed in Tables 1 and 2. <Table 1>

The analysis for the content of free amino acids (A), minerals (B) and heavy metals (C) in the pig placental extract obtained by acidic hydrolysis method

<Table 2>

The analysis for the content of fatty acids in the pig placental extract obtained

by acidic hydrolysis method

Preparation example 2; Preparation of the pig placental extract by the hot water extraction method

1 kg of the washed pig placental was mixed with 500 mL of water and the mixture was hot water extracted at 110°C for about 5 to 12 hours. The obtained hot water extract was passed through 200 mesh filter to firstly remove solids which was not resolved, and was secondly passed through 0.45 μm and 0.2 μm filters in order to remove the remaining solids. Then, the crude protein content of the hot water extract was measured. The hot water extract was condensed or diluted to obtain the pig

placental hot water extract having 0.4 % of the final nitrogen content. The obtained the pig placental hot water extract was mix with excipient (corncob) in the ratio of 1 : 1 and the mixture was dried to the water content of 10%, and then it was added to the feed of mouse in amount of 0.2 wt% as the feed additive. The content of the pig placental hot water extract are showed in Tables 3 to 5.

Preparation example 3: Preparation of the pig placental extract by the alkaline hydrolysis method

1 kg of the washed pig placental was mixed with 500 mL of 3N NaOH and the mixture was hydrolyzed at 110 ^° C for about 5 hours, and the next process was continued after confirming complete hydrolysis of protein by the Burette reaction and the trichroloacetic acid reaction. The content of the crude protein was measured by the Kildal method, and the final nitrogen content was controlled to 0.4 % by the condensation or dilution. Then the hydrolyzed solution of the pig placental was treated with HCl to adjust to pH 10. The hydrolyzed solution of the pig placental of pH 10 was mix with excipient (corncob) in the ratio of 1:1 and the mixture was dried to the water content of 10%, and then it was added to the feed of mouse in amount of 0.2 wt% as the feed additive. The content of hydrolyzed solution of the pig placental is showed in Tables 3 to 5. The process of the alkaline hydrolysis method is illustrated in Figure 2.

Preparation example 4: Preparation of the pig placental powder

The washed pig placental was freeze-dried to control the water content below to 10 %, and the freeze-dried pig placental was pulverized by using the pulverizer and then

it was added to the feed of mouse in amount of 0.2 wt% as the feed additive. The content of the dried pig placental powder are showed in Tables 3 to 5. <Table 3>

The analysis for amino acid contents in the pig placental extract in accordance with the preparation process

<Table 4>

The analysis for mineral contents in the pig placental extract in accordance with the preparation process

<Table 5>

The analysis for fatty acid contents in the pig placental extract in accordance with the preparation process

[Mode for Carrying Out the Invention]

Example 2: The effect of the pig placental extract in accordance with the preparation process on the growth of animal

The effect of animal feed or drinking water containing the pig placental extracts prepared in preparation examples 1 to 4 of example 1 with different process on the growth of animal was examined.

Experimental example 1: The test for feeding of drinking pig placental extract obtained by the acidic hydrolysis method

The pig placental extract prepared in preparation example 1 of example 1 was added in drinking water and was compared its effect with available product A of company B.

Six-week-old ICR mouse (Korea Biolink) was purchased and alleviated for 7 days in breeding room controlling with temperature of 21 to 24 ^° C, moisture of 40±5 % and lighting time of 12 hours. Healthy 30 males and females mice were used in the test. 5 mice were distributed in each mouse cage and bred for 9 days.

60 g of solid feed was supplied to each cage every day. As drinking water, pure water was supplied to controlling group, and the mixture of pure water and 4 wt% of the pig placental extract (PPE; nitrogen content: 0.1%, 0.2% and 0.4%), the human placenta extract(HPE; nitrogen content 0.2%) and product A(aminolite) of company B was supplied to treating group according to the condition. 0.2 mL of drinking water was supplied to one mouse per day.

The test was performed with total 6 mice comprising controlling group (Table 6). Mice were bred with sufficient feed and drinking water for 9 days. Death rate was measured every day during the breeding period. The amount of weight change, feed intake and drinking water intake were measured for 9 days and a variation amount was expressed with value by dividing it by the population, and its effect was analyzed.

In addition, the blood was taken from mouse after finishing the test and the amount of total protein, cholesterol, alkaline phosphatase, blood urine nitrogen(BUN), neutral fat and IgG in blood were measured, and histopathological resion was performed by the autopsy.

<Table 6>

The conditions of the test for feeding of drinking pig placental extract obtained by the acidic hydrolysis method

<Table 7>

The analysis of the growth of ICR mouse according to the change of supplying constituent and concentration under the standard breeding conditions

* Values in Table 7 was expressed as mean values by breeding 5 mice per each condition on sex

As shown in Table 7 and Figure 3, ICR mouse was not dead for 9 days of breeding period. In the result of analysis of daily weight increment and feed demand after the test, the treating group having high nitrogen content in the pig placental extracts which is added in drinking water showed the highest weight increment in all of female and male, and feed demand was also analyzed to have the lowest value, hi case of female, the weight gain in each the treating group of the pig placental extract was increased 1.1, 1.4 and 1.6 times than the controlling group corresponding to 0.1 %, 0.2 % and 0.4 % of nitrogen content respectively. In case of male, which was lower than that of female, the weight gain in each the treating group of the pig placental extract having 0.4% of nitrogen content was increased about 1.3 times than the controlling group. The treating group with the human placental extract (0.2% N) also showed the increased weight gain than the controlling group. In the result of comparison to the treating group with the pig placental extract, female showed the same weight gain in the human placental extract with the treating group with the pig placental extract having similar nitrogen content, however, male showed the somewhat higher weight gain in the human placental extract with the treating group with the pig placental extract.

However, in comparison with the amino acid product A of another company, the treating group with the pig placental extract showed the higher weight gain and feed demand. Moreover, in the result of autopsy after completing the test, any abnormal indication was not showed in both of the treating group and the controlling group.

<Table 8>

Concentration of IgG and cholesterol in accordance with each experimental condition

In the result of analysis for the content of ingredient in among each treating group through serum test, there is no difference in the content of total protein and albumin, alkaline phosphatage, BUN, neutral fat (not showed). As shown in table 8, the content of cholesterol in both of male and female was higher in the treating group than that of the controlling group, and the content of IgG in male was also higher in the treating group than that of the controlling group.

Example 2: The test for the pig placental extract obtained by the acidic hydrolysis method as the feed additive

In order to confirm availability of the pig placental extract as animal feed additive, the pig placental extract having 0.4 % of nitrogen content for the animal feed additive were added into the powdered feed at a fixed ratio and then the feed mixture was dried into the form of solid and supplied.

Experimental example 1: The test for mouse

Three- week-old ICR mouse (Korea Biolink) was purchased and alleviated for 7 days in breeding room controlling with temperature of 21 to 24 ^° C, moisture of 40±5 % and lighting time of 12 hours. Healthy 30 mice were used in the test. The test was performed repeatedly twice according to each condition after distributing 5 mice in each mouse cage. The breeding period was 10 days.

The pig placental extract for the feed additive having 0.4 % of nitrogen content which is prepared in preparation example 1 was mixed with mouse feed at the ratio of 0, 0.4 and 4 wt% and the feed mixture was dried into solid and then 60 g of solid feed was supplied to each cage every day. As drinking water, pure water was sufficiently supplied to all of controlling group and treating group.

The test was performed repeatedly twice with dividing 3 groups comprising controlling group (Table 9). Mice were bred with sufficient feed and drinking water for 10 days. Death rate was measured every day during the breeding period. The amount of weight change, feed intake and drinking water intake after 10 days of breeding were measured and a variation amount was divided by the population (population of twice repetition) and expressed by value, and its effect was analyzed.

In addition, the blood was taken from mouse after finishing the test and the amount of total protein, cholesterol, alkaline phosphatase, blood urine nitrogen(BUN), neutral fat and IgG in blood were measured and its significance different was confirmed. Histopathological resion was performed by the autopsy.

<Table 9>

The conditions of test for the pig placental extract as the feed additive

<Table 10>

Comparison for the growth of mouse according to concentration of the pig placental extract added in feed

As shown in Table 10, in the result of breeding mouse with the feed comprising the pig placental extract for 10 days, the weight increment was highest when 0.4 wt% of the pig placental extract was added into the feed and it is rather reduced than the controlling group when 4 wt% of the pig placental extract was added into the feed.

In the result of confirming the feed demand by using the weight increment and the feed intake, the feed demand was lowest when 0.4 wt% of the pig placental extract was added into the feed (12.02).

<Table 11>

The examination for effect of the pig placental extract on mouse through serum analysis

As shown in Table 11, in the result of serum analysis, the higher content of the pig placental extract in the feed is increased, the higher total protein and albumin is increased and its significance was recognized by statistical processing(p<0.05). hi addition, significance of BUN in the treating group to that of the controlling group was recognized by statistical analysis, however it showed a tendency of decrease in adding the pig placental extract. However, SGOT(Serum Glutamic Oxalate Transaminate), SGPT(Serum Glutamic Pyruvate Transaminate), triglyceride, alkaline phosphatase, total cholesterol and IgG showed a tendency of increase and decrease between the treating group and the controlling group, but there is no significance(p>0.05).

Experimental example 2: The test for pig

The pig placental extract for the feed additive prepared in preparation example 1 was added into the feed for weaning pig and its effect on the growth of pig was examined.

The present test was practiced in the Hanareum farm in Yong-in using weaning pig for 19 days and it was performed three times repeatedly by dividing with 40 pigs of the treating groups which is supplied the feed comprising the pig placental extract and 40 pigs of the controlling group which is supplied the feed without the pig placental extract (total 240 pigs). The pig placental extract having 0.4% of nitrogen content for the feed additive prepared in preparation example 1 was added into the feed for weaning

pig which is supplied adjust to feeding day, in amount of 0.2 % and 0.4 %(w/w) of the feed and then the feed mixture was dried in the form of solid and supplied. After and before 19 days, mean value of the initiation weight and the ending weight in each condition were measured to estimate the daily weight gain and the results were compared.

As shown in Figure 4A, the test was performed in that 80 heads of weaning pig (3 times repetition) which is initiated weaning were divided in two conditions and the feed for weaning pig in which the pig placental extract was added in amount of 0.2 %(w/w) was supplied for 19 days. Ln the result of the test, although the mean initiation weight of the treating group was lower than that of the controlling group in amount of 0.91 kg, the mean ending weight of the treating group was higher than that of the controlling group in amount of 0.08 kg such that the weight gain of the treating group showed higher than that of the controlling group in amount of 0.99 kg.

Moreover, as shown in Figure 4B, the daily weight gain of the treating group which is supplied the pig placental extract was 0.3 kg/day, but that of the controlling group was 0.25 kg/day such that the daily weight gain of the treating group was higher than that the controlling group in amount of about 17%.

As shown in Figures 4C and 4D, in the result of supplying the feed for weaning pig comprising 0.4 %(w/w) of the pig placental extract, the treating group also showed 1.12 kg of the weight such that the daily weight gain of the treating group was higher than that the controlling group in amount of about 19%.

From the above results, it can be known that the pig placental extract increase the growth of weaning pig.

Example 3: The test for the pig placental extract obtained by the hot water extract, the alkaline hydrolysis or the pulverization as the feed additive

In order to confirm availability of the pig placental extract in accordance with conditions of the preparing process as animal feed additive, the pig placental extract or powder having 0.4 % of nitrogen content for the animal feed additive prepared in preparation example 2 to 4 of example 1 were added into the animal feed in amount of 0.2 %(w/w) and then the feed mixture was dried into the form of solid and supplied.

Experimental example 1: The test for mouse

Three-week-old ICR mouse (Korea Biolink) was purchased and alleviated for 7 days in breeding room controlling with temperature of 21 to 24 "C, moisture of 40±5 % and lighting time of 12 hours. Healthy 30 mice were used in the test. The test was performed repeatedly twice according to each condition after distributing 5 mice in each mouse cage. The breeding period was 21 days. 60 g of the feed was supplied to each cage every day. As drinking water, pure water was sufficiently supplied to all of controlling group and treating group.

The test was performed repeatedly twice with dividing total 4 groups (Table 12). Mice were bred with sufficient feed and drinking water for 21 days. Death rate was measured every day during the breeding period. The amount of weight change, feed intake and drinking water intake after 21 days of breeding were measured and a variation amount was divided by the population (population of twice repetition) and expressed by value, and its effect was analyzed.

In addition, the blood was taken from mouse after finishing the test and the amount of IgG(Immunoglobulin G) in blood were measured and effect on immunity

was analyzed . Histopathological resion was performed by the autopsy.

<Table 12>

The conditions of test for the pig placental extract as the feed additive in mouse

<Table 13>

The examination for effect of the pig placental extract on mouse through serum analysis

As shown in Figure 5, the treating group which is supplied by the pig placental extract obtained by the alkaline hydrolysis showed the highest daily weight gain of 0.38 g/day, which is about 1.2 times higher than that of the controlling group and is the elevated value of about 18 %.

Moreover, the treating group which is supplied by the pig placental powder as the feed additive can be accomplished a similar result to the pig placental extract obtained by the alkaline hydrolysis since it showed about 15 % of the elevated value of the daily weight gain.

However, as comparing the treating group which is supplied by the pig placental feed additive and the controlling group, even though the treating group showed the enhanced the daily weight gain of 6 %, it is lower than that of other two treating groups.

Such values were similar or higher than that of resulted in the test for the pig placental extract obtained by the acidic hydrolysis in which daily weight gain was enhanced is about 8 %, which showed that the acidic hydrolysis as well as three preparation process of the pig placental material can be used in the preparation of the

pig placental feed additive.

In addition, three treating groups which were supplied by the pig placental extract as the feed additive showed the lower feed demand than that of the controlling group, in which the treating groups which was supplied by the pig placental extract obtained by the alkaline hydrolysis showed the lowest feed demand of 12.34 %.

In order to examine the effect of the pig placental extract as the feed additive on the change in immunity of mouse, IgG content in blood was analyzed through the serum analysis.

<Table 14>

The comparison of IgG content of mouse which was supplied by the pig placental extract as the feed additive

As shown in Table 14, the controlling group which was not supplied by the pig placental extract as the feed additive showed values below detection level, however each treating group which were supplied by the pig placental extract as the feed additive were detected IgG besides the first test for the pig placental extract obtained by the hot water extract and the treating group which was supplied by the pig placental extract

obtained by the pulverization, showed the highest IgG content (mean 7.94 mg/dL). In the result of analysis using the statistical program, even though the significance was not showed, it can be considered that the supplement of the pig placental feed additive contributes to the increment of IgG synthesis in blood of mouse to some degree since the treating groups which were supplied by the pig placental feed additive showed the higher IgG content than that of the controlling group.

Experimental example 2: The test for weaning pig

The pig placental extract of powder prepared in preparation examples 2 to 4 was added into the feed according to the weaning pig program of the testing farm and its effect on the growth of pig was examined.

The present test was practiced in the Hanareum farm in Yong-in using weaning pig for 19 days and it was performed two times repeatedly by dividing with three parts of the treating group which was supplied the feed comprising the pig placental extract and one part of the controlling group which is supplied the feed without the pig placental extract. The pig placental feed additive (0.4% of nitrogen content) was added into the feed for weaning pig in amount of 0.2 %(w/w) of the feed adjusting to feeding day. After and before 19 days, the initiation weight and the ending weight in each condition were measured and the results were compared. The above experiment was repeated twice and the mean values were showed in Table 15. <Table 15>

The effect of the pig placental feed additive on the growth of pig

As shown in table 15, there was no dead piglet. The daily weight gain of three treating groups showed higher values than that of the controlling group, in which the treating group supplying the pig placental feed additive prepared by the alkaline hydrolysis showed the highest daily weight gain(0.22 kg/day)(Figure 6). This value is about 1.2 times higher than that of the controlling group, which can be estimated that the supplement of the pig placental feed additive causes to positive effect on the growth of weaning pig.

The feed demand of the treating groups besides the treating group supplying the pig placental feed additive prepared by the hot water extract, showed the low value of about 20-30 %, which means that the feed efficacy was improved.

In general, weaning pig which is starting weaning has to separate from sow to adapt oneself to new circumstances, and is supplied with a weaning food or a feed. Moreover, weaning pig is experienced the contest competition with the new entity in such a new circumstances, by which a stress is caused to weaning pig. The stress is main cause in the illness intrusiveness of pig and therefore it can be considered that just weaning pig exposes to the risk of the illness intrusiveness by the stress. Accordingly, it is very important to manage early weaning pig in pig production and it can have on effect on the final fattening pig shipment. It can be confirmed that the supply of the pig placental feed additive performed in this experiment gave the positive effect on the growth of early weaning pig.

Consequently, all of the method for preparing process of the pig placental using the acidic or alkaline hydrolysis, hot water extract or pulverization can be effectively used in the preparation of the pig placental feed additive, and it could be confirmed that the produced pig placental feed additive have an effect on increasing the growth of pig. [Industrial Availability]

The present invention which relates to the method for preparing the pig placental extract and its use as a animal feed, has an effect on providing the method for preparing the pig placental extract for the animal drinking water additive or feed additive. The pig placental extract of the present invention have on effect on promoting the growth of animal by using as the animal drinking water additive or feed additive. Accordingly, the pig placental extract of the present invention as the animal drinking water or feed

additive can be produced quality meat such that the present invention is very useful in the animal feed industry.