Title:
METHOD OF PREPARING QUEEN BEE EMBRYO PEPTIDES BY ENZYMOLYSIS
Document Type and Number:
WIPO Patent Application WO/2015/100692
Kind Code:
A1
Abstract:
Provided is a method of preparing queen bee embryo peptides by enzymolysis, comprising: pretreating a sample, removing lipids from the queen bee larvae powder, preparing queen bee larvae powder sample liquid, conducting ultrasonic treatment, conducting enzymolysis, placing the enzymatic hydrolysates into boiling water for 20 minutes to deactivate the enzyme, cooling to room temperature, adjusting the PH value of the enzymatic hydrolysates to 7.0, removing the supernatant after centrifuging, obtaining hydrolyzed queen bee embryo peptide powder after drying. The queen bee embryo polypeptides of the invention can be used to produce health care products for improving functions of the human body, and screen biologically active polypeptides effective for the human body.
Inventors:
ZHAO SHUMIN (CN)
BAO YONGGANG (CN)
SHI SONGCHUAN (CN)
BAO YONGGANG (CN)
SHI SONGCHUAN (CN)
Application Number:
PCT/CN2014/000972
Publication Date:
July 09, 2015
Filing Date:
November 05, 2014
Export Citation:
Assignee:
ZHAO SHUMIN (CN)
YU LI (CN)
YU LI (CN)
International Classes:
C12P21/06; G01N21/31
Foreign References:
| CN103725742A | 2014-04-16 | |||
| CN102533913A | 2012-07-04 | |||
| CN102251004A | 2011-11-23 |
Other References:
CHEN, MINGSHENG.: "Health care function research for bioactive peptide of honeybee''.", 2008 NATIONAL BEE PRODUCTS MARKET INFORMATION EXCHANGE CONFERENCE PROCEEDINGS., 31 December 2008 (2008-12-31), pages 81
LIU, XIUJUAN ET AL.: "Technological research for enzymatic hydrolysis of honeybee''.", STORAGE & PROCESS., vol. 06, no. 03, 31 December 2006 (2006-12-31), pages 33
CHEN, MINGSHENG.: "Health care function research for bioactive peptide of honeybee''.", NATIONAL BEE PRODUCTS MARKET INFORMATION EXCHANGE CONFERENCE PROCEEDINGS., 31 December 2008 (2008-12-31), pages 81
LIU, XIUJUAN ET AL.: "Technological research for enzymatic hydrolysis of honeybee''.", STORAGE & PROCESS., vol. 06, no. 03, 31 December 2006 (2006-12-31), pages 33
CHEN, MINGSHENG.: "Health care function research for bioactive peptide of honeybee''.", NATIONAL BEE PRODUCTS MARKET INFORMATION EXCHANGE CONFERENCE PROCEEDINGS., 31 December 2008 (2008-12-31), pages 81
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