Title:
METHOD FOR PREPARING QUIESCENT HEPATIC STELLATE CELL, AND MODEL FOR EVALUATION OF ACTIVATION OF QUIESCENT HEPATIC STELLATE CELL
Document Type and Number:
WIPO Patent Application WO/2020/166726
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for preparing a quiescent hepatic stellate cell by inducing the differentiation of a pluripotent stem cell; and a method for screening for a chemical substance involved in the activation of a quiescent hepatic stellate cell. According to the present invention, a method for preparing a quiescent hepatic stellate cell is provided, which comprises a step of inducing the differentiation of an iPS cell and fractionating a quiescent hepatic stellate cell by employing a NGFR-positive phenotype as an index. Also provided is a screening method for selecting as a candidate substance that can be used as a drug for treating or preventing a fibrosis-related liver disease on the basis of the expression amount of a gene marker that is expressed specifically in a hepatic stellate cell that is activated by introducing a reporter gene into the quiescent hepatic stellate cell.
Inventors:
MIYAJIMA ATSUSHI (JP)
KIDO TAKETOMO (JP)
KOUI YUTA (JP)
KIDO TAKETOMO (JP)
KOUI YUTA (JP)
Application Number:
PCT/JP2020/006147
Publication Date:
August 20, 2020
Filing Date:
February 17, 2020
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12N5/077; C12N5/10; C12Q1/02; C12Q1/6897
Domestic Patent References:
| WO2016148216A1 | 2016-09-22 |
Foreign References:
| CN104232588A | 2014-12-24 |
Other References:
KOUI, Y. ET AL.: "An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells", STEM CELL REPORTS, vol. 9, 8 August 2017 (2017-08-08), pages 490 - 498, XP055694369, DOI: 10.1016/j.stemcr.2017.06.010
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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