Title:
A METHOD OF PREPARING RECOMBINANT STAPHYLOKINASE
Document Type and Number:
WIPO Patent Application WO/1995/027048
Kind Code:
A1
Abstract:
The present invention relates to constructing expression vector pSTE-SAK-1 by using staphylokinase amplified by PCR and prokaryotic expression vector, such as PLY-4, and transforming E. coli with pSTE-SAK-1, and inducing the expression of such gene by heating. The engineered bacteria are propagated by fermenting, and destructed. The r-SAK product is purified from the supernatant by a two-step method which includes ion exchange and gel filtration; the purity and the yield of the staphylokinase of the invention are high, and cost low.
Inventors:
SONG HOUYAN (CN)
Application Number:
PCT/CN1995/000025
Publication Date:
October 12, 1995
Filing Date:
April 03, 1995
Export Citation:
Assignee:
SHANGHAI MEDICAL UNIVERSITY (CN)
SONG HOUYAN (CN)
SONG HOUYAN (CN)
International Classes:
C07K14/31; (IPC1-7): C12N9/48
Domestic Patent References:
| WO1993013209A1 | 1993-07-08 |
Foreign References:
| EP0077664A2 | 1983-04-27 |
Download PDF:
Previous Patent: HIGHLY-PURIFIED RECOMBINANT REVERSE TRANSCRIPTASE
Next Patent: HIGH-ALKALINE PROTEASE AND ITS USE
Next Patent: HIGH-ALKALINE PROTEASE AND ITS USE