Title:
METHOD FOR PREPARING STEM CELL CONSTITUENT EXTRACT AND METHOD FOR PREPARING HAIR LOSS TREATMENT AGENT BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2016/072660
Kind Code:
A1
Abstract:
The present invention relates to a method for preparing a stem cell constituent extract and a method for preparing a hair loss treatment agent by using the same. The method for preparing a stem cell constituent extract, according to the present invention, comprises the steps of: obtaining cultured stem cells from which a medium has been removed; counting the number of stem cells by dispersing the stem cells in an isotonic carrier liquid; removing the carrier liquid and obtaining the stem cells; dispersing the stem cells in a hypotonic extender of an amount determined by considering the number of stem cells and the target concentration; and pulverizing the stem cells in the extender. The method for preparing a hair loss treatment agent, containing a stem cell constituent extract, according to the present invention, comprises the steps of: culturing stem cells in a culture solution; washing the stem cells after removing the culture solution; counting the number of stem cells by dispersing the stem cells in an isotonic carrier liquid; removing the carrier liquid and obtaining the stem cells; dispersing the stem cells in a hypotonic extender of an amount determined by considering the number of stem cells and the target concentration; pulverizing the stem cells in the extender; removing cell membrane residues; and filtering out, from the stem cell extract, only a component having a molecular weight of 3,000-5,000.
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Inventors:
KIM YOUNGSIL (KR)
Application Number:
PCT/KR2015/011467
Publication Date:
May 12, 2016
Filing Date:
October 28, 2015
Export Citation:
Assignee:
TIARA STEM CELL INST (KR)
International Classes:
C12N5/074; A61K8/98; A61K35/545; A61Q7/00; C12N5/02
Foreign References:
| KR20140125735A | 2014-10-29 | |||
| KR20140081762A | 2014-07-01 | |||
| KR20090121541A | 2009-11-26 | |||
| KR20100008763A | 2010-01-26 |
Other References:
PARK, B. -S. ET AL.: "Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion", BIOMEDICAL RESEARCH, vol. 31, no. 1, 2010, pages 27 - 34, XP009158891, DOI: doi:10.2220/biomedres.31.27
RAJASINGH ET AL.: "Cell -free embryonic stem cell extract-mediated derivation of multipotent stem cells from NIH3T3 fibroblasts for functional and anatomical ischemic tissue repair", CIRCULATION RESEARCH, vol. 102, no. 11, 2008, pages e107 - e117
RAJASINGH ET AL.: "Cell -free embryonic stem cell extract-mediated derivation of multipotent stem cells from NIH3T3 fibroblasts for functional and anatomical ischemic tissue repair", CIRCULATION RESEARCH, vol. 102, no. 11, 2008, pages e107 - e117
Attorney, Agent or Firm:
KIM, DAEHYUN (KR)
κΉλν (KR)
κΉλν (KR)
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