Title:
METHOD FOR PRETREATING BIOLOGICAL SAMPLE CONTAINING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/022107
Kind Code:
A1
Abstract:
Provided is a method for pretreating a biological sample in cases where a protein contained in the biological sample is determined using an immunological method.
A method for pretreating a biological sample in cases where a protein contained in the biological sample is determined using an immunological method, said method for pretreating a biological sample being characterized by comprising the following steps:
(1) the biological sample is frozen at a temperature higher than -80°C, in particular, at a temperature of not less than -70°C;
(2) the frozen biological sample is thawed; and
(3) the biological sample is solubilized using a surfactant.
Inventors:
SASAKI SEI (JP)
OHMOTO YASUKAZU (JP)
MORI TOYOKI (JP)
IWATA FUSAKO (JP)
MURAGUCHI MASAHIRO (JP)
OHMOTO YASUKAZU (JP)
MORI TOYOKI (JP)
IWATA FUSAKO (JP)
MURAGUCHI MASAHIRO (JP)
Application Number:
PCT/JP2012/070570
Publication Date:
February 14, 2013
Filing Date:
August 10, 2012
Export Citation:
Assignee:
OTSUKA PHARMA CO LTD (JP)
NAT UNIV CORP TOKYO MED & DENT (JP)
SASAKI SEI (JP)
OHMOTO YASUKAZU (JP)
MORI TOYOKI (JP)
IWATA FUSAKO (JP)
MURAGUCHI MASAHIRO (JP)
NAT UNIV CORP TOKYO MED & DENT (JP)
SASAKI SEI (JP)
OHMOTO YASUKAZU (JP)
MORI TOYOKI (JP)
IWATA FUSAKO (JP)
MURAGUCHI MASAHIRO (JP)
International Classes:
G01N33/543; G01N1/28; G01N33/48; G01N33/53
Domestic Patent References:
| WO2005093415A1 | 2005-10-06 |
Foreign References:
| JPH08178924A | 1996-07-12 | |||
| JPH07267987A | 1995-10-17 | |||
| JPH1160600A | 1999-03-02 | |||
| JP2005539228A | 2005-12-22 |
Other References:
"BIOMARKERS DEFINITIONS WORKING GROUP: BIOMARKERS AND SURROGATE ENDPOINTS: PREFERRED DEFINITIONS AND CONCEPTUAL FRAMEWORK", CLIN PHARMACOL THER, vol. 69, 2001, pages 89 - 95
"Cell Membrane Water Channel, Aquaporin", THE MEDICAL ASSOCIATION OF NIPPON MEDICAL SCHOOL, vol. 5, no. 2, 2009
AGRE P: "The aquaporin water channels", PROC AM THORAC SOC, vol. 3, no. 1, 2006, pages 5 - 13
AGRE P ET AL.: "Aquaporin CHIP: the archetypal molecular water channel", AM. J. PHYSIOL., vol. 265, no. 4, 1993, pages F463 - 76
GONEN T; WALZT: "The structure of aquaporins", Q. REV. BIOPHYS., vol. 39, no. 4, 2006, pages 361 - 96
EIJI ISHIKAWA: "Enzaimu imunoassei wa ko kaihatsu suru (This is How an Enzyme Immunoassay is Developed", July 2003, GAKKAI SHUPPAN SENTA
"Tanpakushitsu jikken handobukku: bunri seisei shitsuryo bunseki kotai sakusei bunshikan sogo sayo kaiseki nado no kihon genri to saishin purotokoru soshuhen (Protein Experiment Handbook - Basic Principles and Current Protocols of Separation/Purification, Mass Spectrometry, Antibody Production, Molec", August 2003, YODOSHA CO., LTD.
SASAKI S ET AL.: "Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct", CLIN. INVEST.., vol. 93, no. 3, 1994, pages 1250 - 1256
See also references of EP 2743696A4
"Cell Membrane Water Channel, Aquaporin", THE MEDICAL ASSOCIATION OF NIPPON MEDICAL SCHOOL, vol. 5, no. 2, 2009
AGRE P: "The aquaporin water channels", PROC AM THORAC SOC, vol. 3, no. 1, 2006, pages 5 - 13
AGRE P ET AL.: "Aquaporin CHIP: the archetypal molecular water channel", AM. J. PHYSIOL., vol. 265, no. 4, 1993, pages F463 - 76
GONEN T; WALZT: "The structure of aquaporins", Q. REV. BIOPHYS., vol. 39, no. 4, 2006, pages 361 - 96
EIJI ISHIKAWA: "Enzaimu imunoassei wa ko kaihatsu suru (This is How an Enzyme Immunoassay is Developed", July 2003, GAKKAI SHUPPAN SENTA
"Tanpakushitsu jikken handobukku: bunri seisei shitsuryo bunseki kotai sakusei bunshikan sogo sayo kaiseki nado no kihon genri to saishin purotokoru soshuhen (Protein Experiment Handbook - Basic Principles and Current Protocols of Separation/Purification, Mass Spectrometry, Antibody Production, Molec", August 2003, YODOSHA CO., LTD.
SASAKI S ET AL.: "Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct", CLIN. INVEST.., vol. 93, no. 3, 1994, pages 1250 - 1256
See also references of EP 2743696A4
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
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