Title:
METHOD FOR PRODUCING APTAMER
Document Type and Number:
WIPO Patent Application WO/2014/010640
Kind Code:
A1
Abstract:
Disclosed is a method for producing an aptamer that binds specifically with a target substance without using SELEX. The method for producing an aptamer includes the chemical synthesis of an aptamer that has a gene sequence coding for a target protein or a base sequence identical to a region containing a G-quadruplex structure present in a control sequence or transcription product of the gene sequence, and that binds with the target protein or a related gene product of the gene sequence, or the chemical synthesis of an aptamer that has 90% or higher sequence identity with the base sequence of the aptamer, contains a G-quadruplex structure, and binds to the target protein to which the aptamer binds, or to the related gene product.
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Inventors:
IKEBUKURO KAZUNORI (JP)
YOSHIDA WATARU (JP)
YOSHIDA WATARU (JP)
Application Number:
PCT/JP2013/068901
Publication Date:
January 16, 2014
Filing Date:
July 10, 2013
Export Citation:
Assignee:
UNIV TOKYO NAT UNIV CORP (JP)
International Classes:
C12N15/115
Other References:
GATT,B. ET AL.: "Nucleic acid aptamers based on the G-quadruplex structure: therapeutic and diagnostic potential.", CURR MED CHEM, vol. 16, 2009, pages 1248 - 1265
SISSI,C. ET AL.: "The evolving world of protein- G-quadruplex recognition: a medicinal chemist's perspective.", BIOCHIMIE, vol. 93, 2011, pages 1219 - 1230
XIAO,J. ET AL.: "A genome-inspired DNA ligand for the affinity capture of insulin and insulin -like growth factor-2.", J SEP SCI, vol. 32, 2009, pages 1654 - 1664
SUN,D. ET AL.: "Facilitation of a structural transition in the polypurine/polypyrimidine tract within the proximal promoter region of the human VEGF gene by the presence of potassium and G-quadruplex-interactive agents.", NUCLEIC ACIDS RES, vol. 33, 2005, pages 6070 - 6080
QIN,Y. ET AL.: "Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4.", NUCLEIC ACIDS RES, vol. 35, 2007, pages 7698 - 7713
XU,Y. ET AL.: "Formation of the G-quadruplex and i-motif structures in retinoblastoma susceptibility genes (Rb).", NUCLEIC ACIDS RES, vol. 34, 2006, pages 949 - 954
YOSHIDA,W. ET AL.: "Aptamer selection based on g4-forming promoter region.", PLOS ONE, vol. 8, June 2013 (2013-06-01), pages E65497
WATARU YOSHIDA ET AL.: "Genome-chu no G-quadruplex Keisei Hairetsu Joho ni Motozuita Biomarker Ketsugo Aptamer no Tansaku", 93RD ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2013) KOEN YOSHISHU III, 8 March 2013 (2013-03-08), pages 935, 3 EL - 16
TAIKI SAITO ET AL.: "Genome-chu no G-quadruplex Kozo Keisei Hairetsu ni Chakumoku shita Aptamer Tansakuho no Kaihatsu", 93RD ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2013) KOEN YOSHISHU III, 8 March 2013 (2013-03-08), pages 935, 3 EL - 15
SISSI,C. ET AL.: "The evolving world of protein- G-quadruplex recognition: a medicinal chemist's perspective.", BIOCHIMIE, vol. 93, 2011, pages 1219 - 1230
XIAO,J. ET AL.: "A genome-inspired DNA ligand for the affinity capture of insulin and insulin -like growth factor-2.", J SEP SCI, vol. 32, 2009, pages 1654 - 1664
SUN,D. ET AL.: "Facilitation of a structural transition in the polypurine/polypyrimidine tract within the proximal promoter region of the human VEGF gene by the presence of potassium and G-quadruplex-interactive agents.", NUCLEIC ACIDS RES, vol. 33, 2005, pages 6070 - 6080
QIN,Y. ET AL.: "Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4.", NUCLEIC ACIDS RES, vol. 35, 2007, pages 7698 - 7713
XU,Y. ET AL.: "Formation of the G-quadruplex and i-motif structures in retinoblastoma susceptibility genes (Rb).", NUCLEIC ACIDS RES, vol. 34, 2006, pages 949 - 954
YOSHIDA,W. ET AL.: "Aptamer selection based on g4-forming promoter region.", PLOS ONE, vol. 8, June 2013 (2013-06-01), pages E65497
WATARU YOSHIDA ET AL.: "Genome-chu no G-quadruplex Keisei Hairetsu Joho ni Motozuita Biomarker Ketsugo Aptamer no Tansaku", 93RD ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2013) KOEN YOSHISHU III, 8 March 2013 (2013-03-08), pages 935, 3 EL - 16
TAIKI SAITO ET AL.: "Genome-chu no G-quadruplex Kozo Keisei Hairetsu ni Chakumoku shita Aptamer Tansakuho no Kaihatsu", 93RD ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2013) KOEN YOSHISHU III, 8 March 2013 (2013-03-08), pages 935, 3 EL - 15
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
Patent business corporation mountain stream international patent firm (JP)
Patent business corporation mountain stream international patent firm (JP)
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