Title:
METHOD FOR PRODUCING ARMORACIA RUSTICANA-DERIVED PEROXIDASE RECOMBINANT PROTEIN USING FILAMENTOUS FUNGI
Document Type and Number:
WIPO Patent Application WO/2013/180208
Kind Code:
A1
Abstract:
Provided is a modified polynucleotide which includes a wild-type base sequence encoding an armoracia rusticana-derived peroxidase polypeptide and a base sequence having at least one different codon, where the used frequency of the modified codon is the used frequency of three kinds of filamentous fungi Humicola, Aspergillus and Trichoderma, and, in the filamentous fungi, the polypeptide to be encoded can be expressed.
Inventors:
YOKOYAMA FUMIKAZU (JP)
OKAKURA KAORU (JP)
INOUE ATSUSHI (JP)
MURASHIMA KOICHIRO (JP)
NAGASATO TOSHIAKI (JP)
YANAI KOJI (JP)
NAKANE AKITAKA (JP)
OKAKURA KAORU (JP)
INOUE ATSUSHI (JP)
MURASHIMA KOICHIRO (JP)
NAGASATO TOSHIAKI (JP)
YANAI KOJI (JP)
NAKANE AKITAKA (JP)
Application Number:
PCT/JP2013/064995
Publication Date:
December 05, 2013
Filing Date:
May 30, 2013
Export Citation:
Assignee:
MEIJI SEIKA PHARMA CO LTD (JP)
International Classes:
C12N15/09; C12N1/15; C12N9/08; C12Q1/28; D06L4/40; G01N33/535
Domestic Patent References:
| WO2013015326A1 | 2013-01-31 | |||
| WO2012098246A1 | 2012-07-26 | |||
| WO2011021616A1 | 2011-02-24 | |||
| WO2005056787A1 | 2005-06-23 | |||
| WO1998011239A1 | 1998-03-19 | |||
| WO2001090375A1 | 2001-11-29 | |||
| WO1997000944A1 | 1997-01-09 |
Foreign References:
| JP2008527985A | 2008-07-31 | |||
| JP2008523829A | 2008-07-10 | |||
| JP2010183872A | 2010-08-26 | |||
| JPH04262785A | 1992-09-18 |
Other References:
SMITH, A. T. ET AL.: "Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme", J. BIOL. CHEM., vol. 265, 1990, pages 13335 - 13343, XP002900903
MORAWSKI, B. ET AL.: "Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris", PROTEIN ENG., vol. 13, no. 5, 2000, pages 377 - 384, XP002158266
MATSUI, T. ET AL.: "High-efficiency secretory production of peroxidase Cla using vesicular transport engineering in transgenic tobacco", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 102, no. 2, 2006, pages 102 - 109, XP028042205
TE'O, V. S. J. ET AL.: "Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei", FEMS MICROBIOLOGY LETTERS, vol. 190, no. 1, 2000, pages 13 - 19, XP002423247
FUJIYAMA ET AL., EUR. J. BIOCHEM, vol. 173, 1988, pages 681 - 687
LIN ET AL., BIOTECHNOL. PROG., vol. 15, 1999, pages 467 - 471
MORAWSKI ET AL., PROTEIN ENGINEERING, vol. 13, 2000, pages 377 - 384
MATSUI ET AL., J. BIOSCI. BIOENG., vol. 102, 2006, pages 102 - 109
EUR. J. BIOCHEM., vol. 173, no. 3, 1988, pages 681 - 687
H. HORIUCHI ET AL., J. BACTERIOL., vol. 170, 1988, pages 272 - 278
J. SAMBROOK; E. F. FRITSCH; T. MANIATLS: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
MORAWSKI, B. ET AL.: "Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris", PROTEIN ENG., vol. 13, no. 5, 2000, pages 377 - 384, XP002158266
MATSUI, T. ET AL.: "High-efficiency secretory production of peroxidase Cla using vesicular transport engineering in transgenic tobacco", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 102, no. 2, 2006, pages 102 - 109, XP028042205
TE'O, V. S. J. ET AL.: "Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei", FEMS MICROBIOLOGY LETTERS, vol. 190, no. 1, 2000, pages 13 - 19, XP002423247
FUJIYAMA ET AL., EUR. J. BIOCHEM, vol. 173, 1988, pages 681 - 687
LIN ET AL., BIOTECHNOL. PROG., vol. 15, 1999, pages 467 - 471
MORAWSKI ET AL., PROTEIN ENGINEERING, vol. 13, 2000, pages 377 - 384
MATSUI ET AL., J. BIOSCI. BIOENG., vol. 102, 2006, pages 102 - 109
EUR. J. BIOCHEM., vol. 173, no. 3, 1988, pages 681 - 687
H. HORIUCHI ET AL., J. BACTERIOL., vol. 170, 1988, pages 272 - 278
J. SAMBROOK; E. F. FRITSCH; T. MANIATLS: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
Patent business corporation cent Crest international patent firm (JP)
Patent business corporation cent Crest international patent firm (JP)
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