Title:
METHOD FOR PRODUCING BASIC L-AMINO ACIDS OR SALTS THEREOF BY FERMENTATION OF AN ENTEROBACTERIACEAE BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2020/138178
Kind Code:
A1
Abstract:
The present invention provides a method for producing a basic L-amino acid such as, for example, L-ornithine L-citrulline, L-arginine, L-histidine, and L-lysine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding a protein having L-methionine/branched-chain amino acid exporter activity.
Inventors:
ZIYATDINOV MIKHAIL KHARISOVICH (RU)
ROSTOVA YULIA GEORGIEVNA (RU)
IGONINA OLGA NIKOLAEVNA (RU)
KNIAZEV ANDREI VIACHESLAVOVICH (RU)
ROSTOVA YULIA GEORGIEVNA (RU)
IGONINA OLGA NIKOLAEVNA (RU)
KNIAZEV ANDREI VIACHESLAVOVICH (RU)
Application Number:
PCT/JP2019/050844
Publication Date:
July 02, 2020
Filing Date:
December 25, 2019
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12P13/08; C12P13/10; C12P13/24
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ECKHARDT T. ET AL., MOL. GEN. GENET., vol. 138, 1975, pages 225 - 232
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BACHMANN, B.J. ET AL.: "E. coli and Salmonella: cellular and molecular biology", 1996, ASM PRESS, article "Derivations and genotypes of some mutant derivatives of E. coli K-12", pages: 2460 - 2488
"Amino Acid Fermentation", 30 May 1986, GAKKAI SHUPPAN CENTER (LTD., pages: 77 - 100
DOROSHENKO V.G. ET AL.: "The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants", PRIKL. BIOCHIM. MIKROBIOL. (RUSSIAN, vol. 49, no. 2, 2013, pages 149 - 154
LIU Q. ET AL.: "YjeH is a novel exporter of L-methionine and branched-chain amino acids in Escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 81, no. 22, 2015, pages 7753 - 7766
DOROSHENKO V. ET AL.: "YddG from Escherichia coli promotes export of aromatic amino acids", FEMS MICROBIOL. LETT., vol. 275, no. 2, 2007, pages 312 - 318, XP055061953, DOI: 10.1111/j.1574-6968.2007.00894.x
LIVSHITS V.A. ET AL.: "Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli", RES. MICROBIOL., vol. 154, no. 2, 2003, pages 123 - 135, XP002464283, DOI: 10.1016/S0923-2508(03)00036-6
BRADFORD M.M., ANAL. BIOCHEM., vol. 72, 1976, pages 248 - 254
LOWRY O.H. ET AL., J. BIOL. CHEM., vol. 193, 1951, pages 265 - 275
"GenBank", Database accession no. NC_000913.3
WHITE T.J. ET AL.: "The polymerase chain reaction", TRENDS GENET., vol. 5, 1989, pages 185 - 189, XP025207915, DOI: 10.1016/0168-9525(89)90073-5
AKHVERDYAN V.Z. ET AL., BIOTECHNOL. (RUSSIAN, vol. 3, 2007, pages 3 - 20
KOMA D. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. 2, 2012, pages 815 - 829
PFLEGER B.F. ET AL.: "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", NAT. BIOTECHNOL., vol. 24, 2006, pages 1027 - 1032, XP055046133, DOI: 10.1038/nbt1226
MUTALIK V.K. ET AL.: "Precise and reliable gene expression via standard transcription and translation initiation elements", NAT. METHODS, vol. 10, 2013, pages 354 - 360
AKHVERDYAN V.Z. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 857 - 871
TYO K.E.J. ET AL., NATURE BIOTECHNOL., vol. 27, 2009, pages 760 - 765
GOLDSTEIN M.A. ET AL.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
GOLD L. ET AL., ANNU. REV. MICROBIOL., vol. 35, 1981, pages 365 - 403
HUI A. ET AL., EMBO J., vol. 3, 1984, pages 623 - 629
DATSENKO K.A.WANNER B.L.: "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645, XP002210218, DOI: 10.1073/pnas.120163297
ZHANG Y. ET AL., NATURE GENET., vol. 20, 1998, pages 123 - 128
BERNARD R. GLICKJACK J. PASTERNAKCHERYL L. PATTEN: "Molecular Biotechnology: principles and applications of recombinant DNA", 2009, ASM PRESS
MANDEL M.HIGA A.: "Calcium-dependent bacteriophage DNA infection", J. MOL. BIOL., vol. 53, 1970, pages 159 - 162, XP024011744, DOI: 10.1016/0022-2836(70)90051-3
MORSE M.L. ET AL.: "Transduction in Escherichia coli K-12", GENETICS, vol. 41, no. 1, 1956, pages 142 - 156
MILLER J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LA. PRESS
MINAEVA N. ET AL., BMC BIOTECHNOLOGY, vol. 8, 2008, pages 63
SAMBROOK J.RUSSELL D.W.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
MOORE S.D.: "Assembling new Escherichia coli strains by Pl-duction", METHODS MOL. BIOL., vol. 765, 2011, pages 155 - 169
QIAN LIU ET AL: "YjeH Is a Novel Exporter of L-Methionine and Branched-Chain AminoAcids in Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 81, no. 22, 28 August 2015 (2015-08-28), US, pages 7753 - 7766, XP055682128, ISSN: 0099-2240, DOI: 10.1128/AEM.02242-15
VOGEL H.J.MACLELLAN W.L., ACETYLORNITHINASE (ESCHERICHIA COLI), METHODS ENZYMOL., vol. 17A, 1970, pages 265 - 269
ECKHARDT T. ET AL., MOL. GEN. GENET., vol. 138, 1975, pages 225 - 232
SAIER M.H. JR. ET AL.: "The Transporter Classification Database (TCDB): recent advances", NUCLEIC ACIDS RES., vol. 44, no. D1, 2016, pages D372 - 9
JACK D.L. ET AL.: "The amino acid/polyamine/organocation (APC) superfamily of transporters specific for amino acids, polyamines and organocations", MICROBIOLOGY, vol. 146, no. 8, 2000, pages 1797 - 1814
SAIER M.H. ET AL.: "The transporter classification database", NUCLEIC. ACIDS RES., vol. 42, no. 1, 2014, pages D251 - 8
BACHMANN, B.J. ET AL.: "E. coli and Salmonella: cellular and molecular biology", 1996, ASM PRESS, article "Derivations and genotypes of some mutant derivatives of E. coli K-12", pages: 2460 - 2488
"Amino Acid Fermentation", 30 May 1986, GAKKAI SHUPPAN CENTER (LTD., pages: 77 - 100
DOROSHENKO V.G. ET AL.: "The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants", PRIKL. BIOCHIM. MIKROBIOL. (RUSSIAN, vol. 49, no. 2, 2013, pages 149 - 154
LIU Q. ET AL.: "YjeH is a novel exporter of L-methionine and branched-chain amino acids in Escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 81, no. 22, 2015, pages 7753 - 7766
DOROSHENKO V. ET AL.: "YddG from Escherichia coli promotes export of aromatic amino acids", FEMS MICROBIOL. LETT., vol. 275, no. 2, 2007, pages 312 - 318, XP055061953, DOI: 10.1111/j.1574-6968.2007.00894.x
LIVSHITS V.A. ET AL.: "Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli", RES. MICROBIOL., vol. 154, no. 2, 2003, pages 123 - 135, XP002464283, DOI: 10.1016/S0923-2508(03)00036-6
BRADFORD M.M., ANAL. BIOCHEM., vol. 72, 1976, pages 248 - 254
LOWRY O.H. ET AL., J. BIOL. CHEM., vol. 193, 1951, pages 265 - 275
"GenBank", Database accession no. NC_000913.3
WHITE T.J. ET AL.: "The polymerase chain reaction", TRENDS GENET., vol. 5, 1989, pages 185 - 189, XP025207915, DOI: 10.1016/0168-9525(89)90073-5
AKHVERDYAN V.Z. ET AL., BIOTECHNOL. (RUSSIAN, vol. 3, 2007, pages 3 - 20
KOMA D. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. 2, 2012, pages 815 - 829
PFLEGER B.F. ET AL.: "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", NAT. BIOTECHNOL., vol. 24, 2006, pages 1027 - 1032, XP055046133, DOI: 10.1038/nbt1226
MUTALIK V.K. ET AL.: "Precise and reliable gene expression via standard transcription and translation initiation elements", NAT. METHODS, vol. 10, 2013, pages 354 - 360
AKHVERDYAN V.Z. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 857 - 871
TYO K.E.J. ET AL., NATURE BIOTECHNOL., vol. 27, 2009, pages 760 - 765
GOLDSTEIN M.A. ET AL.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
GOLD L. ET AL., ANNU. REV. MICROBIOL., vol. 35, 1981, pages 365 - 403
HUI A. ET AL., EMBO J., vol. 3, 1984, pages 623 - 629
DATSENKO K.A.WANNER B.L.: "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645, XP002210218, DOI: 10.1073/pnas.120163297
ZHANG Y. ET AL., NATURE GENET., vol. 20, 1998, pages 123 - 128
BERNARD R. GLICKJACK J. PASTERNAKCHERYL L. PATTEN: "Molecular Biotechnology: principles and applications of recombinant DNA", 2009, ASM PRESS
MANDEL M.HIGA A.: "Calcium-dependent bacteriophage DNA infection", J. MOL. BIOL., vol. 53, 1970, pages 159 - 162, XP024011744, DOI: 10.1016/0022-2836(70)90051-3
MORSE M.L. ET AL.: "Transduction in Escherichia coli K-12", GENETICS, vol. 41, no. 1, 1956, pages 142 - 156
MILLER J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LA. PRESS
MINAEVA N. ET AL., BMC BIOTECHNOLOGY, vol. 8, 2008, pages 63
SAMBROOK J.RUSSELL D.W.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
MOORE S.D.: "Assembling new Escherichia coli strains by Pl-duction", METHODS MOL. BIOL., vol. 765, 2011, pages 155 - 169
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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