Title:
METHOD FOR PRODUCING CARDIOMYOCYTES
Document Type and Number:
WIPO Patent Application WO/2019/151097
Kind Code:
A1
Abstract:
The present invention mainly addresses the problem of providing: a method for producing cardiomyocytes from somatic cells without artificially introducing any genes; or cardiomyocytes obtained from the production method; or a composition that includes a combination of chemical substances that can be used for the production method. The present invention provides, for example: a method for producing cardiomyocytes by directly inducing differentiation of somatic cells, the method characterized by including a step for culturing somatic cells in the presence of an MEK inhibitor and a cAMP inducer; cardiomyocytes obtained from the production method; and a composition for producing cardiomyocytes from somatic cells by directly inducing differentiation, the composition characterized by including an MEK inhibitor and a cAMP inducer. Cardiomyocytes obtained by the present invention are useful in regenerative medicine, etc.
Inventors:
DAI PING (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
Application Number:
PCT/JP2019/002193
Publication Date:
August 08, 2019
Filing Date:
January 24, 2019
Export Citation:
Assignee:
KATAOKA CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
International Classes:
C12N5/077; C12N9/99
Domestic Patent References:
| WO2016056438A1 | 2016-04-14 | |||
| WO2017159463A1 | 2017-09-21 | |||
| WO2018062269A1 | 2018-04-05 |
Foreign References:
| US20160186141A1 | 2016-06-30 | |||
| JP2017104091A | 2017-06-15 | |||
| JP2013524837A | 2013-06-20 | |||
| JP2014525268A | 2014-09-29 | |||
| JP2015213441A | 2015-12-03 | |||
| JP2018013301A | 2018-01-25 | |||
| US20160186141A1 | 2016-06-30 | |||
| US20180062692A1 | 2018-03-01 | |||
| JP2002348243A | 2002-12-04 |
Other References:
CAO, N. ET AL.: "Conversion of human fibroblasts into functional cardiomyocytes by small molecules", SCIENCE, vol. 352, no. 6290, 2016, pages 1216 - 1220, XP055366967, DOI: 10.1126/science.aaf1502
FU, Y. ET AL.: "Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails", CELL RES., vol. 25, no. 9, 2015, pages 1013 - 1024, XP055429604, DOI: 10.1038/cr.2015.99
PING DAI, YUKIMASA TAKEDA: "Direct Reprogramming by Small Molecules for Regenerative Medicine", JOURNAL OF KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, vol. 127, no. 1, 25 January 2018 (2018-01-25), pages 1 - 12, XP009522958, ISSN: 0023-6012
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, vol. 56, no. 3, 2015, pages 166 - 170
CELL RESEARCH, vol. 25, 2015, pages 1013 - 1024
SCIENCE, vol. 352, 2016, pages 1216 - 1220
CHEMICAL ABSTRACTS, Columbus, Ohio, US; abstract no. 2097416-76-5
See also references of EP 3747993A4
FU, Y. ET AL.: "Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails", CELL RES., vol. 25, no. 9, 2015, pages 1013 - 1024, XP055429604, DOI: 10.1038/cr.2015.99
PING DAI, YUKIMASA TAKEDA: "Direct Reprogramming by Small Molecules for Regenerative Medicine", JOURNAL OF KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, vol. 127, no. 1, 25 January 2018 (2018-01-25), pages 1 - 12, XP009522958, ISSN: 0023-6012
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, vol. 56, no. 3, 2015, pages 166 - 170
CELL RESEARCH, vol. 25, 2015, pages 1013 - 1024
SCIENCE, vol. 352, 2016, pages 1216 - 1220
CHEMICAL ABSTRACTS, Columbus, Ohio, US; abstract no. 2097416-76-5
See also references of EP 3747993A4
Attorney, Agent or Firm:
SHIMIZU Naoto (JP)
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