Title:
METHOD FOR PRODUCING CDNA AND RNA CHAINS AND NUCLEOTIDE-IMMOBILIZED SUPPORT
Document Type and Number:
WIPO Patent Application WO/2007/020847
Kind Code:
A1
Abstract:
A method for producing a cDNA chain comprising the steps of: immobilizing a polynucleotide for DNA extension to an insoluble support having on the surface a polymeric substance containing a first unit having a group derived from a phosphoester constituting a hydrophilic region of a phospholipid and a second unit having a carboxylic acid derived group formed by an electron-withdrawing substituent binding to a carbonyl group; bringing a solution containing an RNA fragment, a nucleotide monomer and a reverse transcriptase or an enzyme having a polymerase activity into contact with the surface of the insoluble support; and forming a single-stranded cDNA by extending the polynucleotide for DNA extension by using the RNA fragment in the solution as a template.
Inventors:
KINOSHITA KENJI (JP)
YAKABE TORU (JP)
FUJIMOTO KENTARO (JP)
YOKOYAMA KANEHISA (JP)
FUJIWARA KAZUHIKO (JP)
YAKABE TORU (JP)
FUJIMOTO KENTARO (JP)
YOKOYAMA KANEHISA (JP)
FUJIWARA KAZUHIKO (JP)
Application Number:
PCT/JP2006/315734
Publication Date:
February 22, 2007
Filing Date:
August 09, 2006
Export Citation:
Assignee:
SUMITOMO BAKELITE CO (JP)
KINOSHITA KENJI (JP)
YAKABE TORU (JP)
FUJIMOTO KENTARO (JP)
YOKOYAMA KANEHISA (JP)
FUJIWARA KAZUHIKO (JP)
KINOSHITA KENJI (JP)
YAKABE TORU (JP)
FUJIMOTO KENTARO (JP)
YOKOYAMA KANEHISA (JP)
FUJIWARA KAZUHIKO (JP)
International Classes:
C12N15/00; C12M1/00; C12N15/09; G01N33/53; G01N37/00
Domestic Patent References:
| WO2005029095A1 | 2005-03-31 | |||
| WO2005029095A1 | 2005-03-31 | |||
| WO1993015228A1 | 1993-08-05 |
Foreign References:
| JPH08500722A | 1996-01-30 | |||
| GB2422335A | 2006-07-26 | |||
| EP1820857A1 | 2007-08-22 |
Other References:
See also references of EP 1930422A4
PARK, J. ET AL., ANAL. CHEM., vol. 76, 2004, pages 2649 - 2655
NOBUO IZUMIYA; TETSUO KATO; MICHIHIKO AOYAGI; MICHINORI WAKI: "Pepuchido Gosei no Kiso to Jikken", 1985, MARUZEN CO., LTD
PARK, J. ET AL., ANAL. CHEM., vol. 76, 2004, pages 2649 - 2655
NOBUO IZUMIYA; TETSUO KATO; MICHIHIKO AOYAGI; MICHINORI WAKI: "Pepuchido Gosei no Kiso to Jikken", 1985, MARUZEN CO., LTD
Attorney, Agent or Firm:
HAYAMI, Shinji (2-17-16 Ebisu-Nish, Shibuya-ku Tokyo 21, JP)
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