Title:
METHOD FOR PRODUCING CELL AGGREGATION
Document Type and Number:
WIPO Patent Application WO/2016/121737
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing a cell aggregation using a suspension culture, wherein it is easy to adjust the size of the cell aggregation so as to be appropriate for culturing, and the likelihood of damaging the cells is low. The present invention pertains to a method for producing a cell aggregation that involves a step for culturing cells while suspended in a liquid culture medium containing a lysophospholipid.
Inventors:
HORIGUCHI KAZUKI (JP)
SAKAI YASUYUKI (JP)
IBUKI MASATO (JP)
SAKAI YASUYUKI (JP)
IBUKI MASATO (JP)
Application Number:
PCT/JP2016/052128
Publication Date:
August 04, 2016
Filing Date:
January 26, 2016
Export Citation:
Assignee:
KANEKA CORP (JP)
UNIV TOKYO (JP)
UNIV TOKYO (JP)
International Classes:
C12N5/10; C12N5/0735
Domestic Patent References:
| WO2014133170A1 | 2014-09-04 |
Foreign References:
| JP2008099662A | 2008-05-01 | |||
| JP2006505248A | 2006-02-16 |
Other References:
FRISCA F. ET AL.: "Rho/ROCK pathway is essential to the expansion, differentiation, and morphological rearrangements of human neural stem/progenitor cells induced by lysophosphatidic acid", J LIPID RES, vol. 54, no. 5, May 2013 (2013-05-01), pages 1192 - 1206, XP055472085
BREWER GJ. ET AL.: "Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.", J NEUROSCI RES., vol. 35, no. 5, pages 567 - 76, XP001097842
GARCIA-GONZALO FR . ET AL.: "Albumin-associated lipids regulate human embryonic stem cell self- renewal.", PLOS ONE., vol. 3, no. 1, December 2008 (2008-12-01), pages e1384 ., XP002548861
AVERY K. ET AL.: "Sphingosine-1-phosphate mediates transcriptional regulation of key targets associated with survival, proliferation, and pluripotency in human embryonic stem cells.", STEM CELLS DEV., 17 December 2008 (2008-12-17), pages 1195 - 206, XP055472091
RYU JM. ET AL.: "Sphingosine-1-phosphate-induced Flk-1 transactivation stimulates mouse embryonic stem cell proliferation through S1P1/S1P3-dependent beta-arrestin/c-Src pathways.", STEM CELL RES., vol. 12, no. 1, 7 September 2013 (2013-09-07), pages 69 - 85, XP028671035
See also references of EP 3252153A4
BREWER GJ. ET AL.: "Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.", J NEUROSCI RES., vol. 35, no. 5, pages 567 - 76, XP001097842
GARCIA-GONZALO FR . ET AL.: "Albumin-associated lipids regulate human embryonic stem cell self- renewal.", PLOS ONE., vol. 3, no. 1, December 2008 (2008-12-01), pages e1384 ., XP002548861
AVERY K. ET AL.: "Sphingosine-1-phosphate mediates transcriptional regulation of key targets associated with survival, proliferation, and pluripotency in human embryonic stem cells.", STEM CELLS DEV., 17 December 2008 (2008-12-17), pages 1195 - 206, XP055472091
RYU JM. ET AL.: "Sphingosine-1-phosphate-induced Flk-1 transactivation stimulates mouse embryonic stem cell proliferation through S1P1/S1P3-dependent beta-arrestin/c-Src pathways.", STEM CELL RES., vol. 12, no. 1, 7 September 2013 (2013-09-07), pages 69 - 85, XP028671035
See also references of EP 3252153A4
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
Download PDF:
Previous Patent: ORCHESTRATOR DEVICE, SYSTEM, AND METHOD AND PROGRAM FOR CREATING VIRTUAL MACHINE
Next Patent: METHOD FOR DRUG EVALUATION OF CILOSTAZOL
Next Patent: METHOD FOR DRUG EVALUATION OF CILOSTAZOL