METHOD FOR PRODUCING DNA MOLECULES HAVING AN ADAPTOR SEQUENCE ADDED THERETO, AND USE THEREOF

Title:

METHOD FOR PRODUCING DNA MOLECULES HAVING AN ADAPTOR SEQUENCE ADDED THERETO, AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2021/166989

Kind Code:

A1

Abstract:

The present invention provides: a method for producing DNA molecules having a novel adaptor sequence added thereto; and a use thereof. One embodiment of the present invention provides a method for producing DNA molecules having an adaptor sequence added thereto, the method comprising: a preparation step for preparing double-strand DNA in which a first DNA strand and a second DNA strand are at least partially hybridized; and an annealing step for annealing a partial double-strand oligonucleotide adapter with the 3' end of the first DNA strand of the double-strand DNA. The partial double-strand oligonucleotide adapter is provided with a 3' overhang that is a protruding end including an oligonucleotide having at least eight continuous random or predetermined base sequences and that anneals with the 3' end of the first DNA strand.

More Like This:

JPH0591881	NEW RECOMBINANT DNA MOLECULE ENCODING T CELL ANTIGEN RECEPTOR CHAIN, PREPARATION, ANTIBODY AND MEDICINE CONTAINING THEM
WO/2004/085643	METHODS AND DEVICES FOR PRODUCING BIOMOLECULES
JP6695802	Mutant enzymes and their uses

Inventors:

ICHIHASHI YASUNORI (JP)
HAKOYAMA TSUNEO (JP)

Application Number:

PCT/JP2021/006057

Publication Date:

August 26, 2021

Filing Date:

February 18, 2021

Export Citation:

Click for automatic bibliography generation Help

Assignee:

RIKEN (JP)

International Classes:

C12N15/10; C12Q1/6806; C12Q1/6855; C12Q1/6869; C12Q1/6876; C40B40/06

Foreign References:

JP2018515081A	2018-06-14
JP2016511007A	2016-04-14

Other References:

BRAD T. TOWNSLEY, MICHAEL F. COVINGTON, YASUNORI ICHIHASHI, KRISTINA ZUMSTEIN, NEELIMA R. SINHA: "BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction", FRONTIERS IN PLANT SCIENCE, vol. 6, XP055645033, DOI: 10.3389/fpls.2015.00366
AGARWAL S ET AL.: "Sequencing of first-strand cDNA library reveals full- length transcriptomes", NATURE COMMUNICATIONS, vol. 6, no. 6002, 21 January 2015 (2015-01-21), pages 1 - 12, XP055336676

Attorney, Agent or Firm:

HARAKENZO WORLD PATENT & TRADEMARK (JP)

Download PDF:

View/Download PDF PDF Help

Previous Patent: VEHICLE BODY STRUCTURAL MEMBER AND METHOD FOR DESIGNING VEHICLE BODY STRUCTURAL MEMBER

Next Patent: COMPOUND, THERMOPLASTIC RESIN, OPTICAL MEMBER, AND OPTICAL LENS