Title:
METHOD FOR PRODUCING EXOGENOUS PROTEIN BY USING ESCHERICHIA COLI
Document Type and Number:
WIPO Patent Application WO/2021/200784
Kind Code:
A1
Abstract:
Provided is a method for producing an exogenous protein in a culture solution by means of Escherichia coli, wherein the protein is secreted in the culture solution and can be purified directly from a culture supernatant without applying a load to a post-treatment process after culturing, by suppressing an increase in the viscosity of the culture supernatant. The present invention relates to a method for producing an exogenous protein, wherein Escherichia coli including a gene encoding a peptidoglycan-binding lipoprotein (Pal) having a partial mutation and a gene encoding an exogenous protein is cultured, and said exogenous protein is secreted in the culture supernatant.
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Inventors:
TOKUNAGA TOMOHISA (JP)
NISHIYAMA TOZO (JP)
ISHIGURO KOJIRO (JP)
MINAKUCHI KAZUNOBU (JP)
NISHIYAMA TOZO (JP)
ISHIGURO KOJIRO (JP)
MINAKUCHI KAZUNOBU (JP)
Application Number:
PCT/JP2021/013185
Publication Date:
October 07, 2021
Filing Date:
March 29, 2021
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N15/31; C12P21/02
Domestic Patent References:
| WO2020007493A1 | 2020-01-09 |
Other References:
CASCALES, E. ET AL.: "Deletion analyses of the peptidoglycan- associated lipoprotein Pal reveals three independent binding sequences including a TolA bo x", MOLECULAR MICROBIOLOGY, vol. 51, no. 3, 2004, pages 873 - 885, XP055562131, DOI: 10.1046/j.1365-2958.2003.03881.x
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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