Title:
METHOD OF PRODUCING HIGH-PURITY 2-PYRONE-4,6-DICARBOXYLIC ACID AND METHOD OF PRODUCING INTERMEDIATE FOR SAME
Document Type and Number:
WIPO Patent Application WO/2020/165882
Kind Code:
A3
Abstract:
The present invention relates to a method of producing high-purity 2-pyrone-4,6-dicarboxylic acid (PDC), and a method of producing an intermediate for same, the methods comprising the steps of: 1) producing terephthalic acid (TPA) from PET; 2) inducing TPA to react with a recombinant microorganism strain; and 3) purifying the produced PDC to a high purity. By using a biomass-derived SiO2 catalyst according to the present invention, a preparation method of same, and a method of disintegration of TPA with PET using same, high-purity TPA can be produced at a faster rate than the rate of a conventional PET hydrolysis process under neutral conditions, wherein the TPA thus produced can be usefully utilized as a raw material for polyester fibers and the like. The present invention relates to a recombinant microorganism strain for PDC production, having incorporated therein nucleotide sequences that encode the enzymes TphB, TphAabc, LigAB, and LigC, and to a method of producing PDC, comprising a step of inducing the recombinant microorganism strain for PDC production to react with a substrate. The present invention provides a method of purifying PDC to a high purity by adding acetone to industrially produced or commercially available crude PDC or sodium salts thereof, to remove therefrom, by precipitation, impurities that are detectable by NMR and/or TLC analysis methods but difficult to remove or purify, thereafter extracting a resulting solution with an organic solvent and concentrating the solution, and then eluting a resulting concentrate with a solvent mixture of ethyl acetate and hexane by column chromatography.
Inventors:
CHA HYUN GIL (KR)
JOO JEONG CHAN (KR)
KIM HEE TAEK (KR)
KIM KYUNG AN (KR)
PARK JEYOUNG (KR)
KANG MYUNG JONG (KR)
HWANG SUNG YEON (KR)
OH DONG YEOP (KR)
LEE MIN WOO (KR)
JOO JEONG CHAN (KR)
KIM HEE TAEK (KR)
KIM KYUNG AN (KR)
PARK JEYOUNG (KR)
KANG MYUNG JONG (KR)
HWANG SUNG YEON (KR)
OH DONG YEOP (KR)
LEE MIN WOO (KR)
Application Number:
PCT/IB2020/051457
Publication Date:
May 06, 2021
Filing Date:
February 21, 2020
Export Citation:
Assignee:
KOREA RES INST CHEMICAL TECH (KR)
International Classes:
C07D309/38; C12N9/02; C12N15/70; C12P17/06
Foreign References:
| KR101157373B1 | 2012-06-21 | |||
| JP2005278549A | 2005-10-13 | |||
| US9394549B2 | 2016-07-19 | |||
| KR20180019699A | 2018-02-26 |
Other References:
MICHINOBU TSUYOSHI, HISHIDA MASAKIYO, SATO MASAE, KATAYAMA YOSHIHIRO, MASAI EIJI, NAKAMURA MASAYA, OTSUKA YUICHIRO, OHARA SEIJI, S: "Polyexters of 2-Pryone-4,6-Dicarboxylic Acid (PDC) Obtai ned from a Metabolic Intermediate of Lignin.", POLYMER JOURNAL, vol. 40, no. 1, pages 68 - 75, XP009527770, DOI: 10.1295/polymj.PJ2007158
Attorney, Agent or Firm:
DAE-A INTELLECTUAL PROPERTY CONSULTING (KR)
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