Title:
METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELL BY USING CRISPR/CAS SYSTEM
Document Type and Number:
WIPO Patent Application WO/2022/139499
Kind Code:
A1
Abstract:
The present invention relates to a method for producing induced pluripotent stem cells by using a CRISPR/Cas system and, more specifically, to a method for producing induced pluripotent stem cells, the method comprising a step of inserting a reprogramming factor by using a CRSPR/Cas system containing in a safe harbor of somatic cells a delivery means including two guides RNAs and a Cas protein-encoding nucleic acid. The method for producing induced pluripotent stem cells according to the present invention can prevent mutation in an off-target region and integrate a reprogramming factor into an on-target region safely and specifically, and regulates the reprogramming factor with doxycycline to guarantee consistent expression, whereby induced pluripotent stem cells can be effectively produced. In addition, the induced pluripotent stem cells produced by the method exhibit multipotent markers similar to those of human embryonic stem cells and can differentiate into derivatives of all the three germ layers.
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Inventors:
CHOI SANGDUN (KR)
NASIR JAVAID (KR)
NASIR JAVAID (KR)
Application Number:
PCT/KR2021/019696
Publication Date:
June 30, 2022
Filing Date:
December 23, 2021
Export Citation:
Assignee:
UNIV AJOU IND ACADEMIC COOP FOUND (KR)
International Classes:
C12N5/074; C12N9/22; C12N15/10; C12N15/113; C12N15/90
Domestic Patent References:
| WO2018026723A1 | 2018-02-08 | |||
| WO2019169232A1 | 2019-09-06 |
Foreign References:
| KR20200094795A | 2020-08-07 |
Other References:
ESTHER PALOMINO LAGO: "ESTABLISHING A SAFE HARBOR SITE FOR THE INTRODUCTION OF GENETIC INFORMATION IN THE HUMAN CELLS BY THE RECOMBINATION SYSTEM attP/attB", 1 January 2018 (2018-01-01), XP055665410, Retrieved from the Internet
XIULING CHI, ZHENG, RUHONG JIANG, RUBY CHEN TSAI, LING-JIE KONG, ZONGBIN CUI: "A system for site-specific integration of transgenes in mammalian cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 14, no. 7, 25 July 2019 (2019-07-25), pages e0219842, XP055668688, DOI: 10.1371/journal.pone.0219842
XIULING CHI, ZHENG, RUHONG JIANG, RUBY CHEN TSAI, LING-JIE KONG, ZONGBIN CUI: "A system for site-specific integration of transgenes in mammalian cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 14, no. 7, 25 July 2019 (2019-07-25), pages e0219842, XP055668688, DOI: 10.1371/journal.pone.0219842
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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