Title:
METHOD FOR PRODUCING INTESTINAL ENDOCRINE CELLS, PLURIPOTENT STEM CELL-DERIVED INTESTINAL ENDOCRINE CELLS, MEDIUM OR MEDIUM KIT, AND USE OF THE SAME
Document Type and Number:
WIPO Patent Application WO/2023/074648
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for producing intestinal endocrine cells using gut stem cells induced from pluripotent stem cells; a method for analyzing a disease mechanism; a method for evaluating an anaylte; a therapeutic agent screening method for digestive disorders; pluripotent stem cell-derived intestinal endocrine cells; a medium or a medium kit; and a kit for producing intestinal endocrine cells. The present invention provides a method for producing intestinal endocrine cells, the method including culturing gut stem cells or gut epidermal cells induced from pluripotent stem cells using a culture medium that does not substantially include a ligand of epidermal growth factor receptor but includes a cAMP activating agent and a DNA methyl transferase inhibitor.
Inventors:
YAMAZAKI NAO (JP)
MIMA SHINJI (JP)
IMAKURA YUKI (JP)
MIMA SHINJI (JP)
IMAKURA YUKI (JP)
Application Number:
PCT/JP2022/039602
Publication Date:
May 04, 2023
Filing Date:
October 25, 2022
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/071; C12N5/10; C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
| WO2020091020A1 | 2020-05-07 | |||
| WO2019156200A1 | 2019-08-15 | |||
| WO2017120543A1 | 2017-07-13 | |||
| WO2020091020A1 | 2020-05-07 | |||
| WO2014165663A1 | 2014-10-09 |
Foreign References:
| JP2008206510A | 2008-09-11 | |||
| JP2019527068A | 2019-09-26 | |||
| JP2011517560A | 2011-06-16 |
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TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM J B ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM J B ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM C HMOON J I ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL I PMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
no. 1135306-29-4
CIBELLI ET AL., SCIENCE, vol. 280, 1998, pages 1256 - 1147
WILMUT ET AL., NATURE, vol. 385, 1997, pages 810
IRIYA ET AL., PROTEIN NUCLEIC ACID ENZYME, vol. 44, 1999, pages 892
BAGUISI ET AL., NATURE BIOTECHNOLOGY, vol. 17, 1999, pages 456
WAKAYAMA ET AL., NATURE, vol. 394, 1998, pages 369
NATURE GENETICS, vol. 22, 1999, pages 127
PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 14984
RIDEOUT III ET AL., NATURE GENETICS, vol. 24, 2000, pages 109
TACHIBANA ET AL.: "Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer", CELL, 2013
YU J ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAKAJIMA ET AL., STEM CELLS, vol. 25, 2007, pages 983 - 985
KIM ET AL., CELL STEM CELL, vol. 1, 2007, pages 346 - 352
REVAZOVA ET AL., CLONING STEM CELLS, vol. 9, 2007, pages 432 - 449
REVAZOVA ET AL., CLONING STEM CELLS, vol. 10, 2008, pages 11 - 24
STRELCHENKO N. ET AL., REPROD BIOMED ONLINE, vol. 9, 2004, pages 623 - 629
KLIMANSKAYA I. ET AL., NATURE, vol. 444, 2006, pages 481 - 485
CHUNG Y ET AL., CELL STEM CELL, vol. 2, 2008, pages 113 - 117
ZHANG X. ET AL., STEM CELLS, vol. 24, 2006, pages 2669 - 2676
WASSARMAN, P. M. ET AL., METHODS IN ENZYMOLOGY, vol. 365, 2003
MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 9523, 1998, pages 13726 - 13731
TURNPENNY ET AL., STEM CELLS, vol. 21, no. 5, 2003, pages 598 - 609
TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM J B ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM J B ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM C HMOON J I ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL I PMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
no. 1135306-29-4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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