Title:
METHOD FOR PRODUCING ISOPRENE MONOMER
Document Type and Number:
WIPO Patent Application WO/2015/080273
Kind Code:
A1
Abstract:
Provided is a biological method for improving isoprene productivity. More specifically, provided are: an isoprene synthase-expressing microorganism that shows improved expression of pyrophosphate phosphatase; and a method for producing an isoprene monomer, said method comprising culturing the isoprene synthase-expressing microorganism in a culture medium and thus synthesizing the isoprene monomer. The aforesaid microorganism may have an ability to synthesize dimethylallyl diphosphate via a methylerythritol phosphate pathway, or an ability to synthesize dimethylallyl diphosphate via a mevalonate pathway.
Inventors:
TAJIMA YOSHINORI (JP)
OONUKI AKIKO (JP)
RACHI HIROAKI (JP)
OONUKI AKIKO (JP)
RACHI HIROAKI (JP)
Application Number:
PCT/JP2014/081645
Publication Date:
June 04, 2015
Filing Date:
November 28, 2014
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N1/21; C12N15/09; C12P5/00
Domestic Patent References:
| WO2012088462A1 | 2012-06-28 | |||
| WO2013179722A1 | 2013-12-05 | |||
| WO2010031076A2 | 2010-03-18 | |||
| WO2014052054A1 | 2014-04-03 | |||
| WO2009076676A2 | 2009-06-18 | |||
| WO2010031062A1 | 2010-03-18 | |||
| WO2010027022A1 | 2010-03-11 | |||
| WO2007037460A1 | 2007-04-05 | |||
| WO2013069634A1 | 2013-05-16 | |||
| WO2008090770A1 | 2008-07-31 | |||
| WO2011087139A2 | 2011-07-21 |
Foreign References:
| JP2013216850A | 2013-10-24 | |||
| JP2011505841A | 2011-03-03 | |||
| JP2012516677A | 2012-07-26 | |||
| JP2011505841A | 2011-03-03 | |||
| JP2011518564A | 2011-06-30 | |||
| EP0952221A2 | 1999-10-27 | |||
| JP2005058827A | 2005-03-10 | |||
| RU2006134574A | 2008-04-10 | |||
| US20100062496A1 | 2010-03-11 | |||
| US7919284B2 | 2011-04-05 | |||
| US20140113344A1 | 2014-04-24 |
Other References:
MARY C. WILDERMUTH ET AL.: "Biochemical Characterization of Stromal and Thylakoid-Bound Isoforms of Isoprene Synthase in Willow Leaves", PLANT PHYSIOLOGY, vol. 116, 1998, pages 1111 - 1123, XP055346242
GARY M. SILVER; RAY FALL, PLANT PHYSIOL., vol. 97, 1991, pages 1588 - 1591
MARY C. WILDERMUTH; RAY FALL, PLANT PHYSIOL., vol. 116, 1998, pages 1111 - 1123
KUKKO-KALSKE E. ET AL., J. BACTERIOL., vol. 171, 1989, pages 4498 - 4500
KARLIN; ALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
METHODS ENZYMOL., vol. 183, 1990, pages 63
OLINS P. O. ET AL., GENE, vol. 73, 1988, pages 227 - 235
EVOLUTION, vol. 67, no. 4, 2013, pages 1026 - 1040
BIOTECHNOL. BIOENG., vol. 98, no. 2, 27 March 2007 (2007-03-27), pages 340 - 348
FUJISAKI ET AL., BIOCHEM., vol. 99, 1986, pages 1137 - 1146
DEBOER ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 80, 1983, pages 21 - 25
WU ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 75, 1978, pages 442 - 5446
APPL. MICROBIOL. BIOTECHNOL., vol. 90, 2011, pages 1915 - 1922
SCIENCE, vol. 277, 1997, pages 1453 - 1474
SACCHAROMYCES GENOME DATABASE, Retrieved from the Internet
NUCLEIC ACIDS RES., vol. 40, January 2012 (2012-01-01), pages D700 - D705
MOL GEN GENET., vol. 183, no. 2, 1981, pages 380 - 7
PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645
SAMBROOK, J. ET AL.: "Molecular Cloning: Laboratory Manuals", 1989, COLD SPRING HARBOR LABORATORY PRESS
J. BACTERIOL., vol. 182, no. 15, 2000, pages 4319 - 4327
MINAEVA NI ET AL., BMC BIOTECHNOL., vol. 8, 2008, pages 63
KATASHKINA JI ET AL., BMC MOL BIOL., vol. 10, 2009, pages 34
ANDREEVA IG ET AL., FEMS MICROBIOL LETT., vol. 318, no. 1, 2011, pages 55 - 60
BMC MOL BIOL., vol. 10, 2009, pages 34
WILDING, EI ET AL., J. BACTERIOL., vol. 182, no. 15, 2000, pages 4319 - 4327
TAYLOR LA; ROSE RE, NUCLEIC ACIDS RES., vol. 16, 1988, pages 358
KATASHKINA JI ET AL., BMC MOL. BIOL., vol. 10, 2009, pages 34
HARA Y ET AL.: "The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential", APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. L, January 2012 (2012-01-01), pages 331 - 41
See also references of EP 3075843A4
GARY M. SILVER; RAY FALL, PLANT PHYSIOL., vol. 97, 1991, pages 1588 - 1591
MARY C. WILDERMUTH; RAY FALL, PLANT PHYSIOL., vol. 116, 1998, pages 1111 - 1123
KUKKO-KALSKE E. ET AL., J. BACTERIOL., vol. 171, 1989, pages 4498 - 4500
KARLIN; ALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
METHODS ENZYMOL., vol. 183, 1990, pages 63
OLINS P. O. ET AL., GENE, vol. 73, 1988, pages 227 - 235
EVOLUTION, vol. 67, no. 4, 2013, pages 1026 - 1040
BIOTECHNOL. BIOENG., vol. 98, no. 2, 27 March 2007 (2007-03-27), pages 340 - 348
FUJISAKI ET AL., BIOCHEM., vol. 99, 1986, pages 1137 - 1146
DEBOER ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 80, 1983, pages 21 - 25
WU ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 75, 1978, pages 442 - 5446
APPL. MICROBIOL. BIOTECHNOL., vol. 90, 2011, pages 1915 - 1922
SCIENCE, vol. 277, 1997, pages 1453 - 1474
SACCHAROMYCES GENOME DATABASE, Retrieved from the Internet
NUCLEIC ACIDS RES., vol. 40, January 2012 (2012-01-01), pages D700 - D705
MOL GEN GENET., vol. 183, no. 2, 1981, pages 380 - 7
PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645
SAMBROOK, J. ET AL.: "Molecular Cloning: Laboratory Manuals", 1989, COLD SPRING HARBOR LABORATORY PRESS
J. BACTERIOL., vol. 182, no. 15, 2000, pages 4319 - 4327
MINAEVA NI ET AL., BMC BIOTECHNOL., vol. 8, 2008, pages 63
KATASHKINA JI ET AL., BMC MOL BIOL., vol. 10, 2009, pages 34
ANDREEVA IG ET AL., FEMS MICROBIOL LETT., vol. 318, no. 1, 2011, pages 55 - 60
BMC MOL BIOL., vol. 10, 2009, pages 34
WILDING, EI ET AL., J. BACTERIOL., vol. 182, no. 15, 2000, pages 4319 - 4327
TAYLOR LA; ROSE RE, NUCLEIC ACIDS RES., vol. 16, 1988, pages 358
KATASHKINA JI ET AL., BMC MOL. BIOL., vol. 10, 2009, pages 34
HARA Y ET AL.: "The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential", APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. L, January 2012 (2012-01-01), pages 331 - 41
See also references of EP 3075843A4
Attorney, Agent or Firm:
SAKAI, Hiroaki et al. (JP)
Hiroaki Sakai (JP)
Hiroaki Sakai (JP)
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