Title:
METHOD FOR PRODUCING L-METHIONINE USING A BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2020/067487
Kind Code:
A1
Abstract:
The present invention provides a method for producing L-methionine by fermentation using a bacterium which has been modified to overexpress a 1st gene and a 2nd gene, such as genes having the nucleotide sequences shown in SEQ ID NOs: 1 and 3.
Inventors:
ZIYATDINOV MIKHAIL KHARISOVICH (RU)
LABANOV ANDREY OLEGOVICH (RU)
LABANOV ANDREY OLEGOVICH (RU)
Application Number:
PCT/JP2019/038308
Publication Date:
April 02, 2020
Filing Date:
September 27, 2019
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12P13/12; C12N15/63
Domestic Patent References:
| WO2019212052A1 | 2019-11-07 | |||
| WO1996015246A1 | 1996-05-23 | |||
| WO1995016042A1 | 1995-06-15 | |||
| WO2002010209A1 | 2002-02-07 | |||
| WO2008075483A1 | 2008-06-26 | |||
| WO2000018935A1 | 2000-04-06 | |||
| WO1995034672A1 | 1995-12-21 |
Foreign References:
| US20080311632A1 | 2008-12-18 | |||
| US20020110877A1 | 2002-08-15 | |||
| EP2695940A1 | 2014-02-12 | |||
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| US20060216796A1 | 2006-09-28 | |||
| EP0685555A1 | 1995-12-06 | |||
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| US5661012A | 1997-08-26 | |||
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| US20100297716A1 | 2010-11-25 |
Other References:
DATABASE UniProt [online] 16 September 2015 (2015-09-16), "Uncharacterized protein, gene PAJ_0966", XP002796203, retrieved from Pantoea ananatis (strain AJ13355) Database accession no. A0A0H3KVF7
DATABASE UniProt [online] 12 September 2018 (2018-09-12), "Uncharacterized protein DUF1852, ORF Names:C7427_101866", XP002796204, retrieved from Pantoea ananas (Erwinia uredovora) Database accession no. A0A2U1DL34
DATABASE UniProt [online] 18 May 2010 (2010-05-18), "MetE", XP002796205, retrieved from Pantoea ananatis (strain LMG 20103) Database accession no. D4GCV9
DATABASE UniProt [online] 12 September 2018 (2018-09-12), "Methionine synthase (B12-independent), gene C7431_101217", XP002796206, retrieved from Pantoea allii Database accession no. A0A2V2BG75
DATABASE UniProt [online] 25 October 2017 (2017-10-25), "Protein RarD", XP002796207, retrieved from Pantoea sp. VS1 Database accession no. A0A246NHM8
SAIER M.H. JR. ET AL.: "The Transporter Classification Database (TCDB): recent advances", NUCLEIC ACIDS RES., vol. 44, no. D1, 2016, pages D372 - 9
BACHMANN, B.J. ET AL.: "E. coli and Salmonella: cellular and molecular biology", 1996, ASM PRESS, article "Derivations and genotypes of some mutant derivatives of E. coli K-12", pages: 2460 - 2488
KATASHKINA J.I. ET AL.: "Use of the X Red-recombineering method for genetic engineering of Pantoea ananatis", BMC MOL. BIOL., vol. 10, 2009, pages 34, XP021048298, DOI: 10.1186/1471-2199-10-34
LEWIN B.: "Genes VIII", 2004, PEARSON EDUCATION, INC.
WHITE T.J. ET AL.: "The polymerase chain reaction", TRENDS GENET., vol. 5, 1989, pages 185 - 189, XP025207915, DOI: 10.1016/0168-9525(89)90073-5
BRADFORD M.M., ANAL. BIOCHEM., vol. 72, 1976, pages 248 - 254
LOWRY O.H. ET AL., J. BIOL. CHEM., vol. 193, 1951, pages 265 - 275
AKHVERDYAN V.Z. ET AL., BIOTECHNOL. (RUSSIAN, vol. 3, 2007, pages 3 - 20
KOMA D. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. 2, 2012, pages 815 - 829
PFLEGER B.F. ET AL.: "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", NAT. BIOTECHNOL., vol. 24, 2006, pages 1027 - 1032, XP055046133, DOI: 10.1038/nbt1226
MUTALIK V.K. ET AL.: "Precise and reliable gene expression via standard transcription and translation initiation elements", NAT. METHODS, vol. 10, 2013, pages 354 - 360
AKHVERDYAN V.Z. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 857 - 871
TYO K.E.J. ET AL., NATURE BIOTECHNOL., vol. 27, 2009, pages 760 - 765
GOLDSTEIN M.A.DOI R.H.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
GOLD L. ET AL., ANNU. REV. MICROBIOL., vol. 35, 1981, pages 365 - 403
HUI A. ET AL., EMBO J., vol. 3, 1984, pages 623 - 629
BERNARD R. GLICKJACK J. PASTERNAKCHERYL L. PATTEN: "Molecular Biotechnology: principles and applications of recombinant DNA", 2009, ASM PRESS
MANDEL M.HIGA A.: "Calcium-dependent bacteriophage DNA infection", J. MOL. BIOL., vol. 53, 1970, pages 159 - 162, XP024011744, DOI: 10.1016/0022-2836(70)90051-3
MORSE M.L. ET AL.: "Transduction in Escherichia coli K-12", GENETICS, vol. 41, no. 1, 1956, pages 142 - 156
MILLER J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LA. PRESS
DATSENKO K.A.WANNER B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645
ZHANG Y. ET AL., NATURE GENET., vol. 20, 1998, pages 123 - 128
MINAEVA N.I. ET AL., BMC BIOTECHNOLOGY, vol. 8, 2008, pages 63
ELIZABETH C. TYLER ET AL., COMPUTERS AND BIOMEDICAL RESEARCH, vol. 24, no. 1, 1991, pages 72 - 96
BARTON G.J. ET AL., J. MOL. BIOL., vol. 198, no. 2, 1987, pages 327 - 337
THOMPSON JD ET AL., NUCLEIC ACID RES., vol. 22, no. 22, 1994, pages 4673 - 4680
HIGUCHI, R.: "PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.FRITS, H.J., METHODS ENZYMOL., vol. 154, 1987, pages 367
CARRIER T.A.KEASLING J.D., BIOTECHNOL. PROG., vol. 15, 1999, pages 58 - 64
CHATTOPADHYAY M.K. ET AL.: "Control of methionine biosynthesis in Escherichia coli K12: a closer study with analogue-resistant mutants", J. GEN. MICROBIOL., vol. 137, no. 3, 1991, pages 685 - 691, XP009041956
USUDA Y.KURAHASHI O.: "Effects of deregulation of methionine biosynthesis on methionine excretion in Escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 71, no. 6, 2005, pages 3228 - 3234, XP055039220, DOI: 10.1128/AEM.71.6.3228-3234.2005
NAKAMURA T. ET AL.: "Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium", J. BACTERIOL., vol. 158, no. 3, 1984, pages 1122 - 1127
SAMBROOK J.RUSSELL D.W.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
DATABASE UniProt [online] 12 September 2018 (2018-09-12), "Uncharacterized protein DUF1852, ORF Names:C7427_101866", XP002796204, retrieved from Pantoea ananas (Erwinia uredovora) Database accession no. A0A2U1DL34
DATABASE UniProt [online] 18 May 2010 (2010-05-18), "MetE", XP002796205, retrieved from Pantoea ananatis (strain LMG 20103) Database accession no. D4GCV9
DATABASE UniProt [online] 12 September 2018 (2018-09-12), "Methionine synthase (B12-independent), gene C7431_101217", XP002796206, retrieved from Pantoea allii Database accession no. A0A2V2BG75
DATABASE UniProt [online] 25 October 2017 (2017-10-25), "Protein RarD", XP002796207, retrieved from Pantoea sp. VS1 Database accession no. A0A246NHM8
SAIER M.H. JR. ET AL.: "The Transporter Classification Database (TCDB): recent advances", NUCLEIC ACIDS RES., vol. 44, no. D1, 2016, pages D372 - 9
BACHMANN, B.J. ET AL.: "E. coli and Salmonella: cellular and molecular biology", 1996, ASM PRESS, article "Derivations and genotypes of some mutant derivatives of E. coli K-12", pages: 2460 - 2488
KATASHKINA J.I. ET AL.: "Use of the X Red-recombineering method for genetic engineering of Pantoea ananatis", BMC MOL. BIOL., vol. 10, 2009, pages 34, XP021048298, DOI: 10.1186/1471-2199-10-34
LEWIN B.: "Genes VIII", 2004, PEARSON EDUCATION, INC.
WHITE T.J. ET AL.: "The polymerase chain reaction", TRENDS GENET., vol. 5, 1989, pages 185 - 189, XP025207915, DOI: 10.1016/0168-9525(89)90073-5
BRADFORD M.M., ANAL. BIOCHEM., vol. 72, 1976, pages 248 - 254
LOWRY O.H. ET AL., J. BIOL. CHEM., vol. 193, 1951, pages 265 - 275
AKHVERDYAN V.Z. ET AL., BIOTECHNOL. (RUSSIAN, vol. 3, 2007, pages 3 - 20
KOMA D. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 93, no. 2, 2012, pages 815 - 829
PFLEGER B.F. ET AL.: "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", NAT. BIOTECHNOL., vol. 24, 2006, pages 1027 - 1032, XP055046133, DOI: 10.1038/nbt1226
MUTALIK V.K. ET AL.: "Precise and reliable gene expression via standard transcription and translation initiation elements", NAT. METHODS, vol. 10, 2013, pages 354 - 360
AKHVERDYAN V.Z. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 857 - 871
TYO K.E.J. ET AL., NATURE BIOTECHNOL., vol. 27, 2009, pages 760 - 765
GOLDSTEIN M.A.DOI R.H.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
GOLD L. ET AL., ANNU. REV. MICROBIOL., vol. 35, 1981, pages 365 - 403
HUI A. ET AL., EMBO J., vol. 3, 1984, pages 623 - 629
BERNARD R. GLICKJACK J. PASTERNAKCHERYL L. PATTEN: "Molecular Biotechnology: principles and applications of recombinant DNA", 2009, ASM PRESS
MANDEL M.HIGA A.: "Calcium-dependent bacteriophage DNA infection", J. MOL. BIOL., vol. 53, 1970, pages 159 - 162, XP024011744, DOI: 10.1016/0022-2836(70)90051-3
MORSE M.L. ET AL.: "Transduction in Escherichia coli K-12", GENETICS, vol. 41, no. 1, 1956, pages 142 - 156
MILLER J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LA. PRESS
DATSENKO K.A.WANNER B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, no. 12, 2000, pages 6640 - 6645
ZHANG Y. ET AL., NATURE GENET., vol. 20, 1998, pages 123 - 128
MINAEVA N.I. ET AL., BMC BIOTECHNOLOGY, vol. 8, 2008, pages 63
ELIZABETH C. TYLER ET AL., COMPUTERS AND BIOMEDICAL RESEARCH, vol. 24, no. 1, 1991, pages 72 - 96
BARTON G.J. ET AL., J. MOL. BIOL., vol. 198, no. 2, 1987, pages 327 - 337
THOMPSON JD ET AL., NUCLEIC ACID RES., vol. 22, no. 22, 1994, pages 4673 - 4680
HIGUCHI, R.: "PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.FRITS, H.J., METHODS ENZYMOL., vol. 154, 1987, pages 367
CARRIER T.A.KEASLING J.D., BIOTECHNOL. PROG., vol. 15, 1999, pages 58 - 64
CHATTOPADHYAY M.K. ET AL.: "Control of methionine biosynthesis in Escherichia coli K12: a closer study with analogue-resistant mutants", J. GEN. MICROBIOL., vol. 137, no. 3, 1991, pages 685 - 691, XP009041956
USUDA Y.KURAHASHI O.: "Effects of deregulation of methionine biosynthesis on methionine excretion in Escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 71, no. 6, 2005, pages 3228 - 3234, XP055039220, DOI: 10.1128/AEM.71.6.3228-3234.2005
NAKAMURA T. ET AL.: "Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium", J. BACTERIOL., vol. 158, no. 3, 1984, pages 1122 - 1127
SAMBROOK J.RUSSELL D.W.: "Molecular Cloning: A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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