Title:
METHOD FOR PRODUCING L-PENICILLAMINE
Document Type and Number:
WIPO Patent Application WO/2023/176487
Kind Code:
A1
Abstract:
The production method according to the present invention is characterized in that a D-amino acid oxidase used in step 2 in the scheme shown below is a polypeptide selected from the below-mentioned polypeptides (a) to (c). According to the production method, L-penicillamine can be produced from D-penicillamine with high efficiency without the need of performing racemization and optical resolution. (a) A polypeptide which comprises the amino acid sequence represented by SEQ ID NO:2; (b) a polypeptide which comprises an amino acid sequence having such a structure that one or more amino acid residues other than a valine residue at position-230 and a phenylalanine residue at position-242 in the amino acid sequence represented by SEQ ID NO:2 are substituted, added, inserted or deleted, and has a D-amino acid oxidase activity; and (c) a polypeptide which comprises an amino acid sequence having 90% or higher sequence identity to the amino acid sequence represented by SEQ ID NO:2 (provided that a valine residue at position-230 and a phenylalanine residue at position-242 in SEQ ID NO:2 are conserved in the amino acid) and has a D-amino acid oxidase activity.
Inventors:
IWAMOTO YUICHI (JP)
IWASAKI AKIRA (JP)
IWASAKI AKIRA (JP)
Application Number:
PCT/JP2023/007888
Publication Date:
September 21, 2023
Filing Date:
March 02, 2023
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12P13/04; A61K31/198; C07C319/06; C07C323/58; C07D277/06; C07D277/12; C12N9/06; C12N15/53
Foreign References:
| CN113307754A | 2021-08-27 | |||
| CN112500323A | 2021-03-16 |
Other References:
DATABASE UNIPROTKB ANONYMOUS : "A0A452J134 · A0A452J134_9SAUR", XP093092941, retrieved from UNIPROT
BEARD, T. M. ET AL.: "Deracemisation and stereoinversion of alpha-amino acids using D-amino acid oxidase and hydride reducing agents", CHEMICAL COMMUNICATIONS, vol. 3, 2002, pages 246 - 247, XP002257817, DOI: 10.1039/b107580m
YOUNG DOUGLAS W., SEN PRABIR K., MORECOMBE DONALD J.: "The Stereochemistry of beta-Lactam Formation in Penicillin Biosynthesis", EUROPEAN JOURNAL OF BIOCHEMISTRY, PUBLISHED BY SPRINGER-VERLAG ON BEHALF OF THE FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES, vol. 75, no. 1, 1 May 1977 (1977-05-01), pages 133 - 147, XP093092938, ISSN: 0014-2956, DOI: 10.1111/j.1432-1033.1977.tb11511.x
DATABASE UNIPROTKB ANONYMOUS : "A0A1L8HZZ7 · A0A1L8HZZ7_XENLA", XP093092942, retrieved from UNIPROT
BEARD, T. M. ET AL.: "Deracemisation and stereoinversion of alpha-amino acids using D-amino acid oxidase and hydride reducing agents", CHEMICAL COMMUNICATIONS, vol. 3, 2002, pages 246 - 247, XP002257817, DOI: 10.1039/b107580m
YOUNG DOUGLAS W., SEN PRABIR K., MORECOMBE DONALD J.: "The Stereochemistry of beta-Lactam Formation in Penicillin Biosynthesis", EUROPEAN JOURNAL OF BIOCHEMISTRY, PUBLISHED BY SPRINGER-VERLAG ON BEHALF OF THE FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES, vol. 75, no. 1, 1 May 1977 (1977-05-01), pages 133 - 147, XP093092938, ISSN: 0014-2956, DOI: 10.1111/j.1432-1033.1977.tb11511.x
DATABASE UNIPROTKB ANONYMOUS : "A0A1L8HZZ7 · A0A1L8HZZ7_XENLA", XP093092942, retrieved from UNIPROT
Attorney, Agent or Firm:
USFI PATENT ATTORNEYS INTERNATIONAL OFFICE (JP)
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