Title:
METHOD FOR PRODUCING ORGANIC COMPOUND
Document Type and Number:
WIPO Patent Application WO/2012/105711
Kind Code:
A1
Abstract:
[Problem] To provide a simple and extremely efficient method for producing an organic compound by the enzyme method with which it is possible to bond in a desired bonding direction. [Solution] A method for producing an organic compound, characterized in that the method comprises a step for preparing an arbitrary compound and a nucleophilic compound and a step for bringing the arbitrary compound into contact with the nucleophilic compound in the presence of an enzyme or enzyme group having activity in terms of thioesterification of the arbitrary compound, wherein the nucleophilic compound is one that bonds by nucleophilic attack of the thioester moiety of the arbitrary compound.
Inventors:
KINO KUNIKI (JP)
NAKAGAWA TOMOKI (JP)
SATO MASARU (JP)
NAKAGAWA TOMOKI (JP)
SATO MASARU (JP)
Application Number:
PCT/JP2012/052626
Publication Date:
August 09, 2012
Filing Date:
February 06, 2012
Export Citation:
Assignee:
UNIV WASEDA (JP)
KINO KUNIKI (JP)
NAKAGAWA TOMOKI (JP)
SATO MASARU (JP)
KINO KUNIKI (JP)
NAKAGAWA TOMOKI (JP)
SATO MASARU (JP)
International Classes:
C12P1/00; C12P21/02
Other References:
DIECKMANN R. ET AL.: "Dipeptide synthesis by an isolated adenylate-forming domain of non-ribosomal peptide synthetases (NRPS).", FEES LETT., vol. 498, no. 1, 2001, pages 42 - 5
RALF DIECKMANN ET AL.: "Expression of an active adenylate-forming domain of peptide synthetases corresponding to acyl-CoA-synthetases", FEBS LETTERS, vol. 357, 1995, pages 212 - 216
R WECKERMANN ET AL.: "Complete nucleotide sequence of the tycA gene coding the tyrocidine synthetase 1 from Bacillus brevis.", NUCLEIC ACIDS RES., vol. 16, no. 24, 1988, pages 11841
EVA PFEIFER ET AL.: "Characterization of tyrocidine synthetase 1 (TY1): Requirement of posttranslational modification for peptide biosynthesis", BIOCHEMISTRY, vol. 34, no. 22, 1995, pages 7450 - 7459
RALF DIECKMANN ET AL.: "Probing the domain structure and ligand-induced conformational changes by limited proteolysis of tyrocidine synthetase 1", J.MOL.BIOL., vol. 288, 1999, pages 129 - 140
RALF DIECKMANN ET AL.: "Expression of an active adenylate-forming domain of peptide synthetases corresponding to acyl-CoA-synthetases", FEBS LETTERS, vol. 357, 1995, pages 212 - 216
R WECKERMANN ET AL.: "Complete nucleotide sequence of the tycA gene coding the tyrocidine synthetase 1 from Bacillus brevis.", NUCLEIC ACIDS RES., vol. 16, no. 24, 1988, pages 11841
EVA PFEIFER ET AL.: "Characterization of tyrocidine synthetase 1 (TY1): Requirement of posttranslational modification for peptide biosynthesis", BIOCHEMISTRY, vol. 34, no. 22, 1995, pages 7450 - 7459
RALF DIECKMANN ET AL.: "Probing the domain structure and ligand-induced conformational changes by limited proteolysis of tyrocidine synthetase 1", J.MOL.BIOL., vol. 288, 1999, pages 129 - 140
Attorney, Agent or Firm:
YAGUCHI, Taro et al. (JP)
Taro Yaguchi (JP)
Taro Yaguchi (JP)
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Claims: