Title:
METHOD FOR PRODUCING POLYHYDROXYALKANOATE
Document Type and Number:
WIPO Patent Application WO/2023/210998
Kind Code:
A1
Abstract:
Disclosed is a method for producing a polyhydroxyalkanoate (PHA). The disclosed method for producing a PHA may comprise the steps of: partially dissolving a microbial biomass containing a PHA through a pretreatment process using a nonionic surfactant, and obtaining a pretreated biomass from the microbial biomass; obtaining a mixture in which the PHA is dissolved by dissolving the PHA of the pretreated biomass through a solvent extraction process using a non-polar, non-halogen solvent; forming a PHA precipitate by adding a precipitant to the mixture so as to precipitate the PHA; and recovering the non-halogen solvent from the mixture from which the PHA precipitate has been removed. The nonionic surfactant may comprise polyethylene glycol sorbitan monolaurate. The non-halogen solvent may comprise dimethyl carbonate (DMC).
Inventors:
KIM HEUNG DO (KR)
YOON HONG SUB (KR)
YOON HONG SUB (KR)
Application Number:
PCT/KR2023/004562
Publication Date:
November 02, 2023
Filing Date:
April 05, 2023
Export Citation:
Assignee:
YUNBIOTECH CO LTD (KR)
International Classes:
C12P7/625
Domestic Patent References:
| WO2013016566A1 | 2013-01-31 |
Foreign References:
| US20050287654A1 | 2005-12-29 |
Other References:
COLOMBO BIANCA, PEREIRA JOANA, MARTINS MARGARIDA, TORRES-ACOSTA MARIO A., DIAS ANA C.R.V., LEMOS PAULO C., VENTURA SÓNIA P.M., EIS: "Recovering PHA from mixed microbial biomass: Using non-ionic surfactants as a pretreatment step", SEPARATION AND PURIFICATION TECHNOLOGY, ELSEVIER SCIENCE, AMSTERDAM, NL, vol. 253, 1 December 2020 (2020-12-01), NL , pages 117521, XP093102654, ISSN: 1383-5866, DOI: 10.1016/j.seppur.2020.117521
SAMORÌ CHIARA, BASAGLIA MARINA, CASELLA SERGIO, FAVARO LORENZO, GALLETTI PAOLA, GIORGINI LORIS, MARCHI DAVIDE, MAZZOCCHETTI LAURA,: "Dimethyl carbonate and switchable anionic surfactants: two effective tools for the extraction of polyhydroxyalkanoates from microbial biomass", GREEN CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 17, no. 2, 1 January 2015 (2015-01-01), GB , pages 1047 - 1056, XP093102657, ISSN: 1463-9262, DOI: 10.1039/C4GC01821D
MONGILI BEATRICE, ABDEL AZIM ANNALISA, FRATERRIGO GAROFALO SILVIA, BATUECAS ESPERANZA, RE ANGELA, BOCCHINI SERGIO, FINO DEBORA: "Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)", BIOTECHNOLOGY FOR BIOFUELS, vol. 14, no. 1, XP093102659, DOI: 10.1186/s13068-020-01849-y
SAMORÌ CHIARA, BASAGLIA MARINA, CASELLA SERGIO, FAVARO LORENZO, GALLETTI PAOLA, GIORGINI LORIS, MARCHI DAVIDE, MAZZOCCHETTI LAURA,: "Dimethyl carbonate and switchable anionic surfactants: two effective tools for the extraction of polyhydroxyalkanoates from microbial biomass", GREEN CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 17, no. 2, 1 January 2015 (2015-01-01), GB , pages 1047 - 1056, XP093102657, ISSN: 1463-9262, DOI: 10.1039/C4GC01821D
MONGILI BEATRICE, ABDEL AZIM ANNALISA, FRATERRIGO GAROFALO SILVIA, BATUECAS ESPERANZA, RE ANGELA, BOCCHINI SERGIO, FINO DEBORA: "Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)", BIOTECHNOLOGY FOR BIOFUELS, vol. 14, no. 1, XP093102659, DOI: 10.1186/s13068-020-01849-y
Attorney, Agent or Firm:
KIM, Kwonseok (KR)
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