Title:
METHOD OF PRODUCING POLYMER BY NATIVE CHEMICAL LIGATION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/035478
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for producing a peptide-bond-containing polymer in high yield and in a short time. The method for producing a peptide-bond-containing polymer provided by the present invention comprises (A) a step in which a polymer fragment 1 having an unprotected cysteine residue and a polymer fragment 2 having both a protected cysteine residue and any desired activated amino acid residue are linked together by the NCL method to obtain a polymer fragment 1-2 having the protected cysteine residue and (B) a step in which a deprotection agent is added after the step (A) to obtain a polymer fragment 1-2 having unprotected cysteine residues, the step (A) and the step (B) being performed in a reaction system where a deactivator for the deprotection agent is present. Step (A), step (B), and step (C) can be repeated at least once.
Inventors:
OKAMOTO AKIMITSU (JP)
HAYASHI GOSUKE (JP)
KAMO NAOKI (JP)
HAYASHI GOSUKE (JP)
KAMO NAOKI (JP)
Application Number:
PCT/JP2018/030462
Publication Date:
February 21, 2019
Filing Date:
August 17, 2018
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C07K1/06; C07K1/02
Domestic Patent References:
| WO2012110536A1 | 2012-08-23 |
Other References:
TANG, S. ET AL.: "An Efficient One-Pot Four- Segment Condensation Method for Protein Chemical Synthesis", ANGEW. CHEM. INT. ED., vol. 54, no. 19, 4 May 2015 (2015-05-04), pages 5713 - 5717, XP055426013
MONBALIU, JC. ET AL.: "Recent trends in Cys- and Ser/Thr-based synthetic strategies for the elaboration of peptide constructs", CHEM. COMMUN., vol. 48, no. 95, 13 September 2012 (2012-09-13), pages 11601 - 11622, XP055571155
FAUVET, B. ET AL.: "One-pot total chemical synthesis of human alpha-synuclein", CHEM. COMMUN., vol. 49, no. 81, 18 October 2013 (2013-10-18), pages 9254 - 9256, XP055571161
MONBALIU, JC. ET AL.: "Recent trends in Cys- and Ser/Thr-based synthetic strategies for the elaboration of peptide constructs", CHEM. COMMUN., vol. 48, no. 95, 13 September 2012 (2012-09-13), pages 11601 - 11622, XP055571155
FAUVET, B. ET AL.: "One-pot total chemical synthesis of human alpha-synuclein", CHEM. COMMUN., vol. 49, no. 81, 18 October 2013 (2013-10-18), pages 9254 - 9256, XP055571161
Attorney, Agent or Firm:
YOKOTA Nobutaka et al. (JP)
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