Title:
METHOD FOR PRODUCING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2021/251383
Kind Code:
A1
Abstract:
Provided is a method for producing a protein. The method of the present invention comprises culturing Talaromyces cellulolyticus in a medium, said Talaromyces cellulolyticus being capable of producing a target protein and having been modified so as to reduce the activity of protein Pep4, to thereby produce the target protein.
Inventors:
NITTA NOBUHISA (JP)
FUKADA HIROAKI (JP)
SHIMBO KAZUTAKA (JP)
MATSUDAIRA AKIKO (JP)
WATANABE TOSHIKI (JP)
USUDA YOSHIHIRO (JP)
FUKADA HIROAKI (JP)
SHIMBO KAZUTAKA (JP)
MATSUDAIRA AKIKO (JP)
WATANABE TOSHIKI (JP)
USUDA YOSHIHIRO (JP)
Application Number:
PCT/JP2021/021749
Publication Date:
December 16, 2021
Filing Date:
June 08, 2021
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N1/15; C12N15/31; C12P21/02
Domestic Patent References:
| WO2019073954A1 | 2019-04-18 | |||
| WO2019059404A1 | 2019-03-28 | |||
| WO2019073954A1 | 2019-04-18 | |||
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Other References:
LANDOWSKI CHRISTOPHER P., HUUSKONEN ANNE, WAHL RAMON, WESTERHOLM-PARVINEN ANN, KANERVA ANNE, HäNNINEN ANNA-LIISA, SALOVUORI N: "Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei", PLOS ONE, vol. 10, no. 8, pages e0134723, XP055783669, DOI: 10.1371/journal.pone.0134723
DATABASE UniProt [online] 11 December 2019 (2019-12-11), Database accession no. B6Q342_TALMQ
DATABASE UniProt [online] 26 February 2020 (2020-02-26), Database accession no. B8M4Z6_TALSN
INOUE H ET AL.: "Construction of a starch-inducible homologous expression system to produce cellulolytic enzymes from Acremonium cellulolyticus", J IND MICROBIOL BIOTECHNOL, vol. 40, no. 8, August 2013 (2013-08-01), pages 823 - 30, XP035330710, DOI: 10.1007/s10295-013-1286-2
FEMS MICROBIOL. LETT., vol. 351, 2014, pages 32 - 41
GENE, vol. 60, no. 1, 1987, pages 115 - 127
HIGUCHI, R: "PCR Technology", vol. 61, 1989, STOCKTON PRESS
KUNKEL, T.A. ET AL., METH. IN ENZYMOL, vol. 154, 1987, pages 367
CARTER P, METH. IN ENZYMOL, vol. 154, 1987, pages 382
"GenBank", Database accession no. GAM39722.1
MOL GEN GENET, no. 4, 24 June 1996 (1996-06-24), pages 451 - 60
BIOSCI BIOTECHNOL BIOCHEM., vol. 62, no. 12, December 1998 (1998-12-01), pages 2364 - 70
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, no. 5511-5515, 1998
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
"Molecular cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
HASHIMOTO, K ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, 1996, pages 22 - 30
LIN, J. T. ET AL., J. CHROMATOGR. A, vol. 808, 1998, pages 43 - 49
DATABASE UniProt [online] 11 December 2019 (2019-12-11), Database accession no. B6Q342_TALMQ
DATABASE UniProt [online] 26 February 2020 (2020-02-26), Database accession no. B8M4Z6_TALSN
INOUE H ET AL.: "Construction of a starch-inducible homologous expression system to produce cellulolytic enzymes from Acremonium cellulolyticus", J IND MICROBIOL BIOTECHNOL, vol. 40, no. 8, August 2013 (2013-08-01), pages 823 - 30, XP035330710, DOI: 10.1007/s10295-013-1286-2
FEMS MICROBIOL. LETT., vol. 351, 2014, pages 32 - 41
GENE, vol. 60, no. 1, 1987, pages 115 - 127
HIGUCHI, R: "PCR Technology", vol. 61, 1989, STOCKTON PRESS
KUNKEL, T.A. ET AL., METH. IN ENZYMOL, vol. 154, 1987, pages 367
CARTER P, METH. IN ENZYMOL, vol. 154, 1987, pages 382
"GenBank", Database accession no. GAM39722.1
MOL GEN GENET, no. 4, 24 June 1996 (1996-06-24), pages 451 - 60
BIOSCI BIOTECHNOL BIOCHEM., vol. 62, no. 12, December 1998 (1998-12-01), pages 2364 - 70
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, no. 5511-5515, 1998
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
"Molecular cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
HASHIMOTO, K ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, 1996, pages 22 - 30
LIN, J. T. ET AL., J. CHROMATOGR. A, vol. 808, 1998, pages 43 - 49
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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