Title:
METHOD FOR PRODUCING RECOMBINANT AAV9 VIRION
Document Type and Number:
WIPO Patent Application WO/2022/191168
Kind Code:
A1
Abstract:
[Problem] To disclose a method for producing recombinant adeno-associated virus particles (rAAV virions) having less empty viral contamination. The production method (a) cultures mammalian cells into which an AAV vector has been introduced in medium to release rAAV virions into the medium, (b) recovers a culture supernatant from the medium, and (c) purifies rAAV virions from the recovered culture supernatant using anion column chromatography and affinity column chromatography that uses a material having an affinity for an AAV capsid protein as the stationary phase.
Inventors:
OHTSUKI KOTA (JP)
HIRASHIMA TOMOKI (JP)
HIRASHIMA TOMOKI (JP)
Application Number:
PCT/JP2022/009902
Publication Date:
September 15, 2022
Filing Date:
March 08, 2022
Export Citation:
Assignee:
JAPAN CHEM RES (JP)
International Classes:
C12N15/864; C12N7/01; C12N7/02
Domestic Patent References:
| WO2019241535A2 | 2019-12-19 | |||
| WO2017160360A2 | 2017-09-21 |
Foreign References:
| US20210009964A1 | 2021-01-14 | |||
| JP5956331B2 | 2016-07-27 |
Other References:
AYUSO E., GENE THERAPY., vol. 17, 2010, pages 503 - 510
MARIA S., HUMAN GENE THERAPY METHODS., vol. 28, 2017, pages 101 - 108
NICOLE B., MOLECULAR THERAPY., vol. 6, 2002, pages 678 - 686
ALTSCHUL SF., J MOL. BIOL., vol. 215, 1990, pages 403 - 10
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA., vol. 85, 1988, pages 2444
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482 - 9
MARIA S., HUMAN GENE THERAPY METHODS., vol. 28, 2017, pages 101 - 108
NICOLE B., MOLECULAR THERAPY., vol. 6, 2002, pages 678 - 686
ALTSCHUL SF., J MOL. BIOL., vol. 215, 1990, pages 403 - 10
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA., vol. 85, 1988, pages 2444
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482 - 9
Attorney, Agent or Firm:
YAMAMOTO, Yoshiki et al. (JP)
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