Title:
METHOD FOR PRODUCING REVERSIBLY IMMORTALIZED CELL
Document Type and Number:
WIPO Patent Application WO/2022/097716
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for producing a reversibly immortalized cell, whereby it becomes possible to proliferate a cell having an immortalizing gene introduced therein for a long period without damaging the chromosome in the cell and it also becomes possible to remove the immortalizing gene; and a method for producing a large amount of reversibly immortalized cells which can be cloned and have stabilized quality. According to the present invention, a method for producing a reversibly immortalized cell is provided, the method comprising: a step for introducing a chromosomally unintegrated RNA virus vector which carries at least one immortalizing gene selected from the group consisting of Bmi-1 gene, TERT gene and SV40T gene into a mammalian cell to express the immortalizing gene in the cell; and a step for culturing the cell to proliferate the cell.
Inventors:
OSHIMURA MITSUO (JP)
TABATA TOSHIAKI (JP)
KAZUKI YASUHIRO (JP)
UNO NARUMI (JP)
TABATA TOSHIAKI (JP)
KAZUKI YASUHIRO (JP)
UNO NARUMI (JP)
Application Number:
PCT/JP2021/040757
Publication Date:
May 12, 2022
Filing Date:
November 05, 2021
Export Citation:
Assignee:
TRANS CHROMOSOMICS INC (JP)
NAT UNIV CORP TOTTORI UNIV (JP)
NAT UNIV CORP TOTTORI UNIV (JP)
International Classes:
C12N15/86; C12N5/10
Domestic Patent References:
| WO2010084970A1 | 2010-07-29 | |||
| WO2010008054A1 | 2010-01-21 | |||
| WO2017078176A1 | 2017-05-11 | |||
| WO2015046229A1 | 2015-04-02 |
Foreign References:
| JP2009291157A | 2009-12-17 | |||
| JP2003174870A | 2003-06-24 | |||
| JP2021061773A | 2021-04-22 | |||
| JP3953399B2 | 2007-08-08 | |||
| JP2017221219A | 2017-12-21 | |||
| JP5763340B2 | 2015-08-12 | |||
| JP2020186146A | 2020-11-19 |
Other References:
B AN, H. ET AL.: "Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors", PNAS, vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 14234 - 14239, XP002684868, Retrieved from the Internet DOI: 10.1073/PNAS.1103509108
FANG-YING MENG ET AL.: "Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination", WORLD JOURNAL OF GASTROENTEROLOGY, vol. 20, no. 36, 1 September 2014 (2014-09-01), pages 13119 - 13126
BAN HNISHISHITA NFUSAKI NTABATA TSAEKI KSHIKAMURA MTAKADA NINOUE MHASEGAWA MKAWAMATA S, PROC NATL ACAD SCI USA., vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 14234 - 9
BAN HNISHISHITA NFUSAKI NTABATA TSAEKI KSHIKAMURA MTAKADA NINOUE MHASEGAWA MKAWAMATA S, PROC NATL ACAD SCI U S A., vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 14234 - 9
OKAMOTO ET AL., BBRC, vol. 295, 2002, pages 354
FANG-YING MENG ET AL.: "Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination", WORLD JOURNAL OF GASTROENTEROLOGY, vol. 20, no. 36, 1 September 2014 (2014-09-01), pages 13119 - 13126
BAN HNISHISHITA NFUSAKI NTABATA TSAEKI KSHIKAMURA MTAKADA NINOUE MHASEGAWA MKAWAMATA S, PROC NATL ACAD SCI USA., vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 14234 - 9
BAN HNISHISHITA NFUSAKI NTABATA TSAEKI KSHIKAMURA MTAKADA NINOUE MHASEGAWA MKAWAMATA S, PROC NATL ACAD SCI U S A., vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 14234 - 9
OKAMOTO ET AL., BBRC, vol. 295, 2002, pages 354
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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