Title:
METHOD FOR PRODUCING SYNOVIAL MEMBRANE-DERIVED MESENCHYMAL STEM CELLS AND METHOD FOR PRODUCING CELL PREPARATION FOR JOINT TREATMENT
Document Type and Number:
WIPO Patent Application WO/2022/004879
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for producing synovial membrane-derived mesenchymal stem cells, said method having an increased proliferation rate in order to enable obtaining, from synovial tissue, a sufficient amount of synovial membrane-derived mesenchymal stem cells; a method for producing a cell preparation for joint treatment using said method for producing synovial membrane-derived mesenchymal stem cells; synovial membrane-derived mesenchymal stem cells; and a cell preparation for joint treatment. According to the present invention, the method for producing, from synovial tissue, synovial membrane-derived mesenchymal stem cells comprises treating synovial tissue for at least two hours with an enzyme mixture containing one or more collagenase and one or more neutral proteinase, without sterilization of said synovial tissue.
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Inventors:
SEKIYA ICHIRO (JP)
MIZUNO MITSURU (JP)
KATANO HISAKO (JP)
OZEKI NOBUTAKE (JP)
NAKAMURA KENTARO (JP)
YOSHIDA TOMOMI (JP)
MIZUNO MITSURU (JP)
KATANO HISAKO (JP)
OZEKI NOBUTAKE (JP)
NAKAMURA KENTARO (JP)
YOSHIDA TOMOMI (JP)
Application Number:
PCT/JP2021/025142
Publication Date:
January 06, 2022
Filing Date:
July 02, 2021
Export Citation:
Assignee:
FUJIFILM CORP (JP)
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
International Classes:
C12N5/0775; A61K35/32; A61P19/02
Foreign References:
| JP2020078282A | 2020-05-28 | |||
| JP5928961B2 | 2016-06-01 | |||
| JP5656183B2 | 2015-01-21 |
Other References:
YU ZHU, YUCHEN WANG, BIZENG ZHAO, XIN NIU, BIN HU, QING LI, JUNTAO ZHANG, JIAN DING, YUNFENG CHEN, YANG WANG: "Comparison of exosomes secreted by induced pluripotent stem cell-derived mesenchymal stem cells and synovial membrane-derived mesenchymal stem cells for the treatment of osteoarthritis", STEM CELL RESEARCH & THERAPY, vol. 8, no. 1, 64, 1 December 2017 (2017-12-01), pages 1 - 11, XP055583108, DOI: 10.1186/s13287-017-0510-9
PROCKOP, D. J., SCIENCE, vol. 276, 1997, pages 71 - 4
ZUK, P. A. ET AL., MOL BIOL CELL, vol. 13, 2002, pages 4279 - 95
CAO ET AL., NAT CELL BIOL, vol. 5, 2003, pages 640 - 6
DE BARI, C ET AL., ARTHRITIS RHEUM, vol. 44, 2001, pages 1928 - 42
FUKUMOTO, T ET AL., OSTEOARTHRITIS CARTILAGE, vol. 11, 2003, pages 55 - 64
SAKAGUCHI ET AL., ARTHRITIS RHUM, vol. 52, 2005, pages 2521 - 9
See also references of EP 4177340A4
PROCKOP, D. J., SCIENCE, vol. 276, 1997, pages 71 - 4
ZUK, P. A. ET AL., MOL BIOL CELL, vol. 13, 2002, pages 4279 - 95
CAO ET AL., NAT CELL BIOL, vol. 5, 2003, pages 640 - 6
DE BARI, C ET AL., ARTHRITIS RHEUM, vol. 44, 2001, pages 1928 - 42
FUKUMOTO, T ET AL., OSTEOARTHRITIS CARTILAGE, vol. 11, 2003, pages 55 - 64
SAKAGUCHI ET AL., ARTHRITIS RHUM, vol. 52, 2005, pages 2521 - 9
See also references of EP 4177340A4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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