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Title:
METHOD FOR PRODUCING TRANS-POLYISOPRENOID, VECTOR, TRANSGENIC PLANT, METHOD FOR PRODUCING PNEUMATIC TIRE AND METHOD FOR PRODUCING RUBBER PRODUCT
Document Type and Number:
WIPO Patent Application WO/2018/116726
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for producing a trans-polyisoprenoid by which the productivity of a trans-rubber can be increased. The present invention pertains to: a method for producing a trans-polyisoprenoid in vitro, said method comprising using a gene encoding a protein of the trans-prenyltransferase (tPT) family and utilizing rubber particles to which the protein encoded by the gene is bound; or a method for producing a trans-polyisoprenoid, said method comprising introducing into a plant a vector, which comprises a gene encoding a tPT family protein bound to a promoter having a promoter activity of laticifer-specific gene expression, and thus laticifer-specifically expressing the protein encoded by the gene.

Inventors:
SAKURAI YUKO (JP)
YAMAGUCHI HARUHIKO (JP)
INOUE YUKINO (JP)
FUSHIHARA KAZUHISA (JP)
TAKAHASHI SEIJI (JP)
YAMASHITA SATOSHI (JP)
NAKAYAMA TORU (JP)
Application Number:
PCT/JP2017/041732
Publication Date:
June 28, 2018
Filing Date:
November 21, 2017
Export Citation:
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Assignee:
SUMITOMO RUBBER IND (JP)
UNIV TOHOKU (JP)
UNIV KANAZAWA (JP)
International Classes:
C12N15/09; A01H1/00; A01H5/00; B60C1/00; C12N5/10; C12P5/02
Foreign References:
JP2016154458A2016-09-01
JP2016093186A2016-05-26
JP2016149973A2016-08-22
JP2005312436A2005-11-10
JP2005308412A2005-11-04
Other References:
ANONYMOUS: "Sucessful in vitro synthesis of natural rubber by bioengineering. Contributing to the stable supply of natural rubber and the development of natural rubber with new molecular structure", TOHOKU UNIVERSITY, 16 November 2016 (2016-11-16), XP055606256, Retrieved from the Internet [retrieved on 20180208]
YAMASHITA, S. ET AL.: "Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliens is", ELIFE, vol. 5, 28 October 2016 (2016-10-28), pages e19022, XP055606257, Retrieved from the Internet
PHATTHIYA, A. ET AL.: "Cloning and expression of the gene encoding solanesyl diphosphate synthase from Hevea brasiliens is", PLANT SCIENCE, vol. 172, 2007, pages 824 - 831, XP005908363, DOI: doi:10.1016/j.plantsci.2006.12.015
TAKAHASHI SEIJI ET AL.: "Molecular insights of natural rubber biosynthesis an approach from prenyltransferase gene analysis", THE SOCIETY OF RUBBER SCIENCE AND TECHNOLOGY, vol. 76, no. 12, 2003, Japan, pages 446 - 452, XP055606259
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
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