Title:
METHOD FOR PRODUCING USEFUL SUBSTANCE VIA NOVEL XYLOSE METABOLIC SYSTEM BASED ON IMPROVED IRON METABOLIC ENGINEERING
Document Type and Number:
WIPO Patent Application WO/2021/193666
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a novel method that makes it possible to produce a useful substance more efficiently using yeast. The present invention is a method for producing a xylose metabolic substance, the method being characterized by culturing, in a medium that includes a sulfur-containing compound, recombinant host cells that excessively express a polynucleotide encoding at least one protein which controls biosynthesis of an iron-sulfur cluster and that have a polynucleotide introduced therein, the polynucleotide encoding a group of other-species-derived enzymes involved in a xylose metabolic path. Preferably, the recombinant host cells have a polynucleotide introduced therein, the polynucleotide encoding a molecule that imparts oxidative stress resistance to the recombinant host cells.
Inventors:
HASUNUMA TOMOHISA (JP)
KONDO AKIHIKO (JP)
HIDESE RYOTA (JP)
BAMBA TAKAHIRO (JP)
YUKAWA TAKAHIRO (JP)
KONDO AKIHIKO (JP)
HIDESE RYOTA (JP)
BAMBA TAKAHIRO (JP)
YUKAWA TAKAHIRO (JP)
Application Number:
PCT/JP2021/012073
Publication Date:
September 30, 2021
Filing Date:
March 23, 2021
Export Citation:
Assignee:
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
C12N15/53; C12N15/52; C12P7/18; C12P7/42; C12P7/50; C12N1/19
Domestic Patent References:
| WO2019159831A1 | 2019-08-22 |
Other References:
YAKAWA, TAKAHIRO ET AL.: "Development of a 1, 2, 4-butanetriol production process from xylose based on improvement of an iron-sulfur protein", LECTURE ABSTRACTS OF THE 70TH SBJ ANNUAL MEETING, 7 August 2018 (2018-08-07)
SALUSJARVI L. ET AL.: "Production of ethylene glycol or glycolic acid from D- xylose in Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 101, 2017, pages 8151 - 8163, XP036347977, DOI: 10.1007/s00253-017-8547-3
BORGSTROM C. ET AL.: "Identification of modifications procuring growth on xylose in recombinant Saccharomyces cerevisiae strains carrying the Weimberg pathway", METABOLIC ENGINEERING, vol. 55, 2019, pages 1 - 11, XP085767824, DOI: 10.1016/j.ymben.2019.05.010
AKADA R. ET AL.: "Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae", YEAST, vol. 19, 2002, pages 17 - 28, XP008140064, DOI: 10.1002/yea.797
SLININGER P. J. ET AL.: "Culture Nutrition and Physiology Impact the Inhibitor Tolerance of the Yeast Pichia stipitis NRRL Y-7124", BIOTECHNOLOGY AND BIOENGINEERING, vol. 102, no. 3, 2009, pages 778 - 790, XP071097662, DOI: 10.1002/bit.22110
OSIRO KAREN O., BORGSTRÖM CELINA, BRINK DANIEL P., FJÖLNISDÓTTIR BIRTA LÍF, GORWA-GRAUSLUND MARIE F.: "Exploring the xylose paradox in Saccharomyces cerevisiae through in vivo sugar signalomics of targeted deletants", MICROBIAL CELL FACTORIES, vol. 18, no. 1, 1 December 2019 (2019-12-01), pages 88, XP055862511, DOI: 10.1186/s12934-019-1141-x
WALLACE-SALINAS VALERIA, SIGNORI LORENZO, LI YING-YING, ASK MAGNUS, BETTIGA MAURIZIO, PORRO DANILO, THEVELEIN JOHAN M, BRANDUARDI : "Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate", AMB EXPRESS, vol. 4, no. 1, 1 December 2014 (2014-12-01), XP055862514, DOI: 10.1186/s13568-014-0056-5
SALUSJARVI L. ET AL.: "Production of ethylene glycol or glycolic acid from D- xylose in Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 101, 2017, pages 8151 - 8163, XP036347977, DOI: 10.1007/s00253-017-8547-3
BORGSTROM C. ET AL.: "Identification of modifications procuring growth on xylose in recombinant Saccharomyces cerevisiae strains carrying the Weimberg pathway", METABOLIC ENGINEERING, vol. 55, 2019, pages 1 - 11, XP085767824, DOI: 10.1016/j.ymben.2019.05.010
AKADA R. ET AL.: "Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae", YEAST, vol. 19, 2002, pages 17 - 28, XP008140064, DOI: 10.1002/yea.797
SLININGER P. J. ET AL.: "Culture Nutrition and Physiology Impact the Inhibitor Tolerance of the Yeast Pichia stipitis NRRL Y-7124", BIOTECHNOLOGY AND BIOENGINEERING, vol. 102, no. 3, 2009, pages 778 - 790, XP071097662, DOI: 10.1002/bit.22110
OSIRO KAREN O., BORGSTRÖM CELINA, BRINK DANIEL P., FJÖLNISDÓTTIR BIRTA LÍF, GORWA-GRAUSLUND MARIE F.: "Exploring the xylose paradox in Saccharomyces cerevisiae through in vivo sugar signalomics of targeted deletants", MICROBIAL CELL FACTORIES, vol. 18, no. 1, 1 December 2019 (2019-12-01), pages 88, XP055862511, DOI: 10.1186/s12934-019-1141-x
WALLACE-SALINAS VALERIA, SIGNORI LORENZO, LI YING-YING, ASK MAGNUS, BETTIGA MAURIZIO, PORRO DANILO, THEVELEIN JOHAN M, BRANDUARDI : "Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate", AMB EXPRESS, vol. 4, no. 1, 1 December 2014 (2014-12-01), XP055862514, DOI: 10.1186/s13568-014-0056-5
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES (JP)
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