Title:
METHOD FOR PRODUCING VIRUS-LIKE PARTICLES ON THE BASIS OF POLYOMA VIRUSES
Document Type and Number:
WIPO Patent Application WO1999025837
Kind Code:
A3
Abstract:
The invention relates to a method for producing virus-like particles on the basis of polyoma viruses. The invention can be used in medical fields such as gene therapy, vaccine development and diagnosis as well as in veterinary medicine. The aim of the invention is to develop a method for producing virus-like particles for gene therapy, diagnosis and vaccine development. This method should enable a highly efficient production of non-infectious, endotoxin-free virus-like particles which are suitable for humane applications. The inventive method relates to the application of a yeast high-expression system which permits the production of large quantities of virus-like particles on the basis of the main capsid protein VP1 of polyoma viruses. By extracting said particles from yeast cells, these virus-like particles are endotoxin-free and suitable for humane applications.
Inventors:
SCHERNECK SIEGFRIED (DE)
SASNAUSKAS KESTUTIS (LT)
ULRICH RAINER (DE)
SASNAUSKAS KESTUTIS (LT)
ULRICH RAINER (DE)
Application Number:
PCT/DE1998/003329
Publication Date:
July 15, 1999
Filing Date:
November 13, 1998
Export Citation:
Assignee:
MAX DELBRUECK CENTRUM (DE)
SCHERNECK SIEGFRIED (DE)
SASNAUSKAS KESTUTIS (LT)
ULRICH RAINER (DE)
SCHERNECK SIEGFRIED (DE)
SASNAUSKAS KESTUTIS (LT)
ULRICH RAINER (DE)
International Classes:
A61K48/00; C07K14/025; C07K16/08; C12N7/04; C12N15/37; C12N15/81; A61K39/00; (IPC1-7): C12N15/37; C12N15/81; C12N7/04; C07K14/025
Other References:
FORSTOVA J ET AL: "POLYOMA VIRUS PSEUDOCAPSIDS AS EFFICIENT CARRIERS OF HETEROLOGOUS DNA INTO MAMMALIAN CELLS", HUMAN GENE THERAPY, vol. 6, no. 3, March 1995 (1995-03-01), pages 297 - 306, XP000645479
E.T. GILLOCK ET AL.: "Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system", J. VIROLOGY, vol. 71, no. 4, April 1997 (1997-04-01), AM.SOC.MICROBIOL.,WASHINGTON,US, pages 2857 - 2865, XP002100672
L. MONTROSS ET AL.: "Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1", J. VIROLOGY, vol. 65, no. 9, September 1991 (1991-09-01), AM.SOC.MICROBIOL.,WASHINGTON,US, pages 4991 - 4998, XP002100673
D.M. SALUNKE ET AL.: "Polymorphism in the assembly of polyomavirus capsid protein VP1", BIOPHYSICAL JOURNAL, vol. 56, November 1989 (1989-11-01), pages 887 - 900, XP002100674
D.M. SALUNKE ET AL.: "Self-assembly of purified polyomavirus capsid protein VP1", CELL, vol. 46, 12 September 1986 (1986-09-12), CELL PRESS,CAMBRIDGE,MA,US;, pages 895 - 904, XP002100675
Y. SHISHIDO ET AL.: "Assembly of JC virus-like particles in COS7 cells", J. MEDICAL VIROLOGY, vol. 51, no. 4, April 1997 (1997-04-01), Wiley-Liss, Inc. NY, US, pages 265 - 272, XP002100676
D. CHANG ET AL.: "Self-assembl of the JC virus major capsid protein, VP1, expressed in insect cells", J. GENERAL VIROLOGY, vol. 78, no. 6, June 1997 (1997-06-01), READING, BERKS, GB, pages 1435 - 1439, XP002100677
K.U. JANSEN ET AL.: "Vaccination with yeast-expressed cottentail rabbit papillomyvirus (CRPV) virus-like particles protects rabbits from CRPV-induced papiloma formation", VACCINE, vol. 13, no. 16, 1995, BUTTERWORTH-HEINEMANN LTD, BOSTON, MA, USA, pages 1509 - 1514, XP002100678
M.P. NEEPER ET AL.: "Expression of the major capsid protein of human papillomavirus type 11 in Saccharomyces cerevisiae", GENE, vol. 180, 1996, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 1 - 6, XP002100679
SASAGAWA T ET AL: "SYNTHESIS AND ASSEMBLY OF VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUSES TYPE 6 AND TYPE 16 IN FISSION YEAST SCHIZOSACCHAROMYCES POMBE", VIROLOGY, vol. 206, January 1995 (1995-01-01), pages 126 - 135, XP000615231
K.J. HOFMANN ET AL.: "Sequence dertermination of human papillomavirus type 6a and assembly of virus like particles in Saccharomyces cerevisiae", VIROLOGY, vol. 209, 1995, ACADEMIC PRESS, INC.,NEW YORK, US, pages 506 - 518, XP002100680
R.B. MORELAND AND R.L. GARCEA: "Characterization of a nuclear localization sequence in the polyomavirus capsid protein VP1", VIROLOGY, vol. 185, 1991, ACADEMIC PRESS, INC.,NEW YORK, US, pages 513 - 518, XP002100681
R. ULRICH ET AL.: "Hamster Polyomavirus-encoded proteins: gene cloning, heterologous expression and imunoreactivity", VIRUS GENES, vol. 12, no. 3, 1996, Kluwer Academic Publishers, Boston, Manufactured in The Netherlands, pages 265 - 274, XP002100682
K. SASNAUSKAS ET AL.: "Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae", GENE, vol. 122, 1992, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 207 - 211, XP002100683
DATABASE SCISEARCH ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL; SASNAUSKAS K ET AL: "Yeast cells allow high-level expression and formation of polyomavirus-like particles", XP002100684
E.T. GILLOCK ET AL.: "Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system", J. VIROLOGY, vol. 71, no. 4, April 1997 (1997-04-01), AM.SOC.MICROBIOL.,WASHINGTON,US, pages 2857 - 2865, XP002100672
L. MONTROSS ET AL.: "Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1", J. VIROLOGY, vol. 65, no. 9, September 1991 (1991-09-01), AM.SOC.MICROBIOL.,WASHINGTON,US, pages 4991 - 4998, XP002100673
D.M. SALUNKE ET AL.: "Polymorphism in the assembly of polyomavirus capsid protein VP1", BIOPHYSICAL JOURNAL, vol. 56, November 1989 (1989-11-01), pages 887 - 900, XP002100674
D.M. SALUNKE ET AL.: "Self-assembly of purified polyomavirus capsid protein VP1", CELL, vol. 46, 12 September 1986 (1986-09-12), CELL PRESS,CAMBRIDGE,MA,US;, pages 895 - 904, XP002100675
Y. SHISHIDO ET AL.: "Assembly of JC virus-like particles in COS7 cells", J. MEDICAL VIROLOGY, vol. 51, no. 4, April 1997 (1997-04-01), Wiley-Liss, Inc. NY, US, pages 265 - 272, XP002100676
D. CHANG ET AL.: "Self-assembl of the JC virus major capsid protein, VP1, expressed in insect cells", J. GENERAL VIROLOGY, vol. 78, no. 6, June 1997 (1997-06-01), READING, BERKS, GB, pages 1435 - 1439, XP002100677
K.U. JANSEN ET AL.: "Vaccination with yeast-expressed cottentail rabbit papillomyvirus (CRPV) virus-like particles protects rabbits from CRPV-induced papiloma formation", VACCINE, vol. 13, no. 16, 1995, BUTTERWORTH-HEINEMANN LTD, BOSTON, MA, USA, pages 1509 - 1514, XP002100678
M.P. NEEPER ET AL.: "Expression of the major capsid protein of human papillomavirus type 11 in Saccharomyces cerevisiae", GENE, vol. 180, 1996, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 1 - 6, XP002100679
SASAGAWA T ET AL: "SYNTHESIS AND ASSEMBLY OF VIRUS-LIKE PARTICLES OF HUMAN PAPILLOMAVIRUSES TYPE 6 AND TYPE 16 IN FISSION YEAST SCHIZOSACCHAROMYCES POMBE", VIROLOGY, vol. 206, January 1995 (1995-01-01), pages 126 - 135, XP000615231
K.J. HOFMANN ET AL.: "Sequence dertermination of human papillomavirus type 6a and assembly of virus like particles in Saccharomyces cerevisiae", VIROLOGY, vol. 209, 1995, ACADEMIC PRESS, INC.,NEW YORK, US, pages 506 - 518, XP002100680
R.B. MORELAND AND R.L. GARCEA: "Characterization of a nuclear localization sequence in the polyomavirus capsid protein VP1", VIROLOGY, vol. 185, 1991, ACADEMIC PRESS, INC.,NEW YORK, US, pages 513 - 518, XP002100681
R. ULRICH ET AL.: "Hamster Polyomavirus-encoded proteins: gene cloning, heterologous expression and imunoreactivity", VIRUS GENES, vol. 12, no. 3, 1996, Kluwer Academic Publishers, Boston, Manufactured in The Netherlands, pages 265 - 274, XP002100682
K. SASNAUSKAS ET AL.: "Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae", GENE, vol. 122, 1992, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 207 - 211, XP002100683
DATABASE SCISEARCH ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL; SASNAUSKAS K ET AL: "Yeast cells allow high-level expression and formation of polyomavirus-like particles", XP002100684
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