Title:
METHOD FOR PROMOTING DE NOVO ROOT REGENERATION OF ULTRADIAN RHYTHM-BASED PLANTS
Document Type and Number:
WIPO Patent Application WO/2021/054741
Kind Code:
A1
Abstract:
Ultradian rhythms (URs) have a period of less than 24 hours. However, the regulation and function of these URs are not clear. The inventors of the present invention have confirmed circadian-independent URs having a period of less than 3 hours in excised Arabidopsis leaves. Gene expression and functional analyses suggest that URs are a positive regulator of de
novo root regeneration (DNRR). 4,073 genes, including phytohormone- and DNRR-related genes representing URs have been identified by transcriptomic analysis. The inventors of the present invention have identified regulator of ultradian rhythm (rou) mutants using forward genetic screening and have confirmed that enhancer of ABA co-receptor 1 (EAR1) is essential in UR cycling. rou1 mutants exhibited a lack of URs, and reduced root regeneration and related gene expression. Hormonal treatment changed EAR1 levels in post-transcriptional and post-translational processes, which means that EAR1 mediates dynamic hormone-related gene expression in excised leaves, thus producing URs and promoting DNRR. According to the present invention, it can be confirmed that leaf excision induces URs, leading to DNRR through hormonal regulation, which is a possible survival mechanism in plants.
Inventors:
HONG SUNGHYUN (KR)
NAM HONG GIL (KR)
VU THI QUY (KR)
PARK SUNG JUN (KR)
LIM JUNHYUN (KR)
NAM HONG GIL (KR)
VU THI QUY (KR)
PARK SUNG JUN (KR)
LIM JUNHYUN (KR)
Application Number:
PCT/KR2020/012564
Publication Date:
March 25, 2021
Filing Date:
September 17, 2020
Export Citation:
Assignee:
INST BASIC SCIENCE (KR)
International Classes:
A01H1/06; A01H5/06; A01H6/20; C12N15/82
Foreign References:
| CN105420245B | 2019-03-29 | |||
| CN109879944A | 2019-06-14 |
Other References:
WANG KAI, HE JUNNA, ZHAO YANG, WU TING, ZHOU XIAOFENG, DING YANGLIN, KONG LINGYAO, WANG XIAOJI, WANG YU, LI JIGANG, SONG CHUN-PENG: "EAR1 Negatively Regulates ABA Signaling by Enhancing 2C Protein Phosphatase Activity", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 30, no. 4, 1 April 2018 (2018-04-01), US, pages 815 - 834, XP055795327, ISSN: 1040-4651, DOI: 10.1105/tpc.17.00875
SUN LI RONG, WANG YI BIN, HE SHI BIN, HAO FU SHUN: "Mechanisms for Abscisic Acid Inhibition of Primary Root Growth", PLANT SIGNALING & BEHAVIOR, vol. 13, no. 9, 2 September 2018 (2018-09-02), pages e1500069, XP055795328, DOI: 10.1080/15592324.2018.1500069
ASAD MUHAMMAD, ZAKARI SHAMSU, ZHAO QIAN, ZHOU LUJIAN, YE YU, CHENG FANGMIN: "Abiotic Stresses Intervene with ABA Signaling to Induce Destructive Metabolic Pathways Leading to Death: Premature Leaf Senescence in Plants", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 2, pages 256, XP055795330, DOI: 10.3390/ijms20020256
ZHAO YANG, GAO JINGHUI, IM KIM JEONG, CHEN KONG, BRESSAN RAY A., ZHU JIAN-KANG: "Control of Plant Water Use by ABA Induction of Senescence and Dormancy: An Overlooked Lesson from Evolution", PLANT AND CELL PHSIOLOGY, OXFORD UNIVERSITY PRESS, UK, vol. 58, no. 8, 1 August 2017 (2017-08-01), UK, pages 1319 - 1327, XP055795333, ISSN: 0032-0781, DOI: 10.1093/pcp/pcx086
SUN LI RONG, WANG YI BIN, HE SHI BIN, HAO FU SHUN: "Mechanisms for Abscisic Acid Inhibition of Primary Root Growth", PLANT SIGNALING & BEHAVIOR, vol. 13, no. 9, 2 September 2018 (2018-09-02), pages e1500069, XP055795328, DOI: 10.1080/15592324.2018.1500069
ASAD MUHAMMAD, ZAKARI SHAMSU, ZHAO QIAN, ZHOU LUJIAN, YE YU, CHENG FANGMIN: "Abiotic Stresses Intervene with ABA Signaling to Induce Destructive Metabolic Pathways Leading to Death: Premature Leaf Senescence in Plants", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 2, pages 256, XP055795330, DOI: 10.3390/ijms20020256
ZHAO YANG, GAO JINGHUI, IM KIM JEONG, CHEN KONG, BRESSAN RAY A., ZHU JIAN-KANG: "Control of Plant Water Use by ABA Induction of Senescence and Dormancy: An Overlooked Lesson from Evolution", PLANT AND CELL PHSIOLOGY, OXFORD UNIVERSITY PRESS, UK, vol. 58, no. 8, 1 August 2017 (2017-08-01), UK, pages 1319 - 1327, XP055795333, ISSN: 0032-0781, DOI: 10.1093/pcp/pcx086
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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