METHOD FOR PURIFYING BONE MORPHOGENETIC PROTEIN

WIPO Patent Application WO/2011/142490

A1

The present invention relates to a method for purifying a protein belonging to the TGF-β superfamily, preferably BMP, and more preferably BMP2. In particular, a method for purifying BMP2 according to the present invention reduces and simplifies the steps as compared to conventional methods for purifying BMP2, and thus may bring about a reduction in the cost and time required for purification. In addition, the method for purifying BMP2 according to the present invention increases the final recovery ratio of BMP2 by solving the problems of taking a long time to purify BMP2 and of the final recovery ratio of BMP2 being lowered due to decomposition by protease in the intermediate steps and loss during the purification process as BMP2 is subjected to more and more purification steps. Furthermore, although the purification steps are reduced in the method for purifying BMP2 according to the present invention, the purity of recovered BMP2 is greatly improved using a filtration method, a chromatography method, columns, a buffer solution, and types and concentrations of compounds that are different from those of conventional methods for purifying BMP2, and optimizing the fractionation size of a thin film used in diafiltration.

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SHIM YOUNG-BOCK (KR)
KIM YEONG-SCHICK (KR)
CHOI YON-RAK (KR)
JANG JU-WOONG (KR)

PCT/KR2010/003021

November 17, 2011

May 12, 2010

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KOREA BONE BANK CO LTD (KR)
SHIM YOUNG-BOCK (KR)
KIM YEONG-SCHICK (KR)
CHOI YON-RAK (KR)
JANG JU-WOONG (KR)

C07K1/36; C07K14/51

US5650494A	1997-07-22
US6352972B1	2002-03-05
US7354722B1	2008-04-08
US7365051B2	2008-04-29

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See also references of EP 2407477A4

ORIGIN PATENT & LAW FIRM (KR)
특허법인오리진 (KR)

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