Title:
METHOD FOR PURIFYING POLYPEPTIDE SIMILAR TO GLP-1
Document Type and Number:
WIPO Patent Application WO/2020/114002
Kind Code:
A1
Abstract:
A method for purifying a polypeptide similar to GLP-1, the purification method uses supercritical chromatography and is characterized in that butyl-bonded silica gel is used as a chromatographic column of a stationary phase, an Al phase in a mobile phase is carbon dioxide, and a B phase is an organic solvent; the organic solvent is selected from among methanol, acetonitrile, or a mixture thereof; the temperature of a column is 30-40°C, and linear elution is performed under a certain elution pressure, the elution pressure being linear elution from a column pressure at a column temperature X0.25 to a column pressure at a column temperature X0.3, and the unit of column pressure being MPa. The preparation method is environmentally friendly, and is highly efficient, has high purity and has high yield.
Inventors:
YIN CHUANLONG (CN)
MI PENGCHENG (CN)
ZENG CHI (CN)
TAO ANJIN (CN)
YUAN JIANCHENG (CN)
MI PENGCHENG (CN)
ZENG CHI (CN)
TAO ANJIN (CN)
YUAN JIANCHENG (CN)
Application Number:
PCT/CN2019/101595
Publication Date:
June 11, 2020
Filing Date:
August 20, 2019
Export Citation:
Assignee:
HYBIO PHARMACEUTICAL CO LTD (CN)
International Classes:
C07K1/16; C07K7/16
Domestic Patent References:
| WO2016019587A1 | 2016-02-11 | |||
| WO2003068397A1 | 2003-08-21 |
Foreign References:
| CN105777872A | 2016-07-20 | |||
| CN107929718A | 2018-04-20 | |||
| CN103421092A | 2013-12-04 | |||
| CN105008009A | 2015-10-28 |
Other References:
DAVID TOGNARELLI ET AL: "Rapid Peptide Separation by Supercritical Fluid Chromatography", BIOANALYSIS, vol. 2, no. 1, 31 December 2010 (2010-12-31), pages 5 - 7, XP009521614, ISSN: 1757-6180, DOI: 10.4155/bio.09.165
TIANHUA XU: "High-speed Countercurrent Chromatography for the Preparation of Nosiheptide", FEED RESEARCH, no. 11, 7 June 2015 (2015-06-07), pages 58 - 59, XP009521615, ISSN: 1002-2813
LINGLI QIN ET AL: "Separation and Purification of Ularitide by Preparative-HPLC", DRUGS & CLINIC, vol. 28, no. 1, 30 January 2013 (2013-01-30), pages 37 - 40, XP055713404, ISSN: 1674-5515, DOI: 10.7501/j.issn.1674-5515.2013.01.010
TIANHUA XU: "High-speed Countercurrent Chromatography for the Preparation of Nosiheptide", FEED RESEARCH, no. 11, 7 June 2015 (2015-06-07), pages 58 - 59, XP009521615, ISSN: 1002-2813
LINGLI QIN ET AL: "Separation and Purification of Ularitide by Preparative-HPLC", DRUGS & CLINIC, vol. 28, no. 1, 30 January 2013 (2013-01-30), pages 37 - 40, XP055713404, ISSN: 1674-5515, DOI: 10.7501/j.issn.1674-5515.2013.01.010
Attorney, Agent or Firm:
BEIJING CHENGHUI LAW FIRM (CN)
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