METHOD FOR PURIFYING PROTEIN DERIVED FROM MEMBRANE PROTEIN COMPLEX

Title:

METHOD FOR PURIFYING PROTEIN DERIVED FROM MEMBRANE PROTEIN COMPLEX

Document Type and Number:

WIPO Patent Application WO/2022/220042

Kind Code:

A1

Abstract:

The purpose of the present disclosure is to provide a method for purifying a protein derived from a membrane protein complex by eluting the membrane protein complex in the form of a complex even when the protein complex is formed. The present disclosure relates to a method for purifying a protein derived from a membrane protein complex, the method comprising: a protein-binding step in which a membrane protein complex that has been eluted from a membrane is bound to an antibody using an artificial polymer; a protein-eluting step in which the membrane protein complex that has been bound to the antibody in the protein-binding step is eluted under a condition in which the pH is 6.0 or higher; and an artificial polymer removal step in which the artificial polymer is removed from the membrane protein complex eluted in the protein-eluting step by performing solvent extraction under a condition in which the pH is 7.0 or lower.

Inventors:

YAMAGUCHI HARUHIKO (JP)
INOUE YUKINO (JP)
TAKAHASHI SEIJI (JP)
NAKAYAMA TORU (JP)

Application Number:

PCT/JP2022/013633

Publication Date:

October 20, 2022

Filing Date:

March 23, 2022

Export Citation:

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Assignee:

SUMITOMO RUBBER IND (JP)
UNIV TOHOKU (JP)

International Classes:

C07K1/14; C07K1/22; C07K1/36; C08F22/00; C12N9/10

Domestic Patent References:

WO2017002504A1

2017-01-05

Foreign References:

JP2020510026A	2020-04-02
JP2010509602A	2010-03-25

Other References:

ASAWATRERATANAKUL KASEM ET AL: "Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis: A key factor participating in natural rubber biosynthesis.", EUROPEAN JOURNAL OF BIOCHEMISTRY, PUBLISHED BY SPRINGER-VERLAG ON BEHALF OF THE FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES, vol. 270, no. 23, 1 December 2003 (2003-12-01), pages 4671 - 4680, XP002299740, ISSN: 0014-2956, DOI: 10.1046/j.1432-1033.2003.03863.x