Title:
METHOD FOR QUANTIFYING ODORS, CELLS USED IN SAME, AND METHOD FOR PRODUCING SAID CELLS
Document Type and Number:
WIPO Patent Application WO/2019/035476
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for constructing a device for causing a plurality of receptors to be expressed in respectively different eukaryotic cells upon nucleic acids within through-holes, housing a plurality of eukaryotic cells within the through-holes of the device and causing the receptors to be expressed therein, collectively measuring the degree of activation of the plurality of receptors by a test substance, and evaluating the degree of activation as a specific numerical value. Provided is an array which includes a substrate and a hydrophobic coating film upon the substrate, the hydrophobic coating film having a plurality of through-holes, and a nucleic acid containing a gene which codes for a prescribed receptor being provided in the interior of each through-hole in a manner touching the substrate.
Inventors:
KURODA SHUN'ICHI (JP)
Application Number:
PCT/JP2018/030453
Publication Date:
February 21, 2019
Filing Date:
August 16, 2018
Export Citation:
Assignee:
KOMI HAKKO CORP (JP)
International Classes:
C12N15/09; C12M1/00; C12N1/15; C12N1/19; C12N5/071; C12N5/10; C12N15/12; C12Q1/02; G01N21/64
Domestic Patent References:
| WO2003100057A1 | 2003-12-04 | |||
| WO2015020158A1 | 2015-02-12 | |||
| WO2006123555A1 | 2006-11-23 | |||
| WO2003100057A1 | 2003-12-04 |
Other References:
OH, E. H. ET AL.: "Cell -based high-throughput odorant screening system through visualization on a microwell array", BIOSENSORS AND BIOELECTRONICS, vol. 53, no. 1, 15 March 2014 (2014-03-15), pages 18 - 25, XP055577712
SHIROKOVA, E. ET AL.: "Identification of specific ligands for orphan olfactory receptors", J. BIOL. CHEM., vol. 280, no. 12, 25 March 2005 (2005-03-25), pages 11807 - 11815, XP055577704
NACHE, V. ET AL.: "Deciphering the function of the CNGBlb subunit in olfactory CNG channels", SCIENTIFIC REPORTS, vol. 6, 11 July 2016 (2016-07-11), pages 29378, XP055577701
MIMURA, HIDEYA: "Role of histamine receptor in olfactory nerve", JAPANESE JOURNAL OF RHINOLOGY ( NIHON BIKA GAKKAI KAISHI ), vol. 48, no. 1, 2009, pages 79 - 81, XP055577699
TOUHARA, K. ET AL.: "Functional identification and reconstitution of an odorant receptor in single olfactory neurons", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 4040 - 4045, XP002973354, DOI: doi:10.1073/pnas.96.7.4040
SHIRASU, M. ET AL.: "Olfactory rexeptor and neural pathway responsible for highly selective sensing of musk odors", NEURON, vol. 81, 8 January 2014 (2014-01-08), pages 165 - 178, XP028813092, DOI: doi:10.1016/j.neuron.2013.10.021
SATO-AKUHARA, N. ET AL.: "Ligand specificity and evolution of mammalian musk odor receptors: effect of single receptor deletion on odor detection", THE JOURNAL OF NEUROSCIENCE, vol. 36, no. 16, 20 April 2016 (2016-04-20), pages 4482 - 4491, XP009191169
SUZUKI, M. ET AL.: "Deciphering the receptor repertoire encoding specific odorants by time-lapse single- cell array cytometry", SCIENTIFIC REPORTS, vol. 6, 2016, pages 19934, XP055395721, DOI: doi:10.1038/srep19934
GLIA, vol. 16, 1996, pages 247
YOSHIMOTO ET AL., SCIENTIFIC REPORTS, vol. 3, 2013, pages 1191
"GenBank", Database accession no. AY318460
MAINLAND JD ET AL., NATURE NEUROSCIENCE, vol. 17, 2014, pages 114 - 120
SAITO H ET AL., SCIENCE SIGNALING, vol. 2, 2009, pages ra9
IMAMURA MIHO, KAGAKU TO SEIBUTSU, vol. 50, no. 11, 2012
SHIROKOVA, E. ET AL.: "Identification of specific ligands for orphan olfactory receptors", J. BIOL. CHEM., vol. 280, no. 12, 25 March 2005 (2005-03-25), pages 11807 - 11815, XP055577704
NACHE, V. ET AL.: "Deciphering the function of the CNGBlb subunit in olfactory CNG channels", SCIENTIFIC REPORTS, vol. 6, 11 July 2016 (2016-07-11), pages 29378, XP055577701
MIMURA, HIDEYA: "Role of histamine receptor in olfactory nerve", JAPANESE JOURNAL OF RHINOLOGY ( NIHON BIKA GAKKAI KAISHI ), vol. 48, no. 1, 2009, pages 79 - 81, XP055577699
TOUHARA, K. ET AL.: "Functional identification and reconstitution of an odorant receptor in single olfactory neurons", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 4040 - 4045, XP002973354, DOI: doi:10.1073/pnas.96.7.4040
SHIRASU, M. ET AL.: "Olfactory rexeptor and neural pathway responsible for highly selective sensing of musk odors", NEURON, vol. 81, 8 January 2014 (2014-01-08), pages 165 - 178, XP028813092, DOI: doi:10.1016/j.neuron.2013.10.021
SATO-AKUHARA, N. ET AL.: "Ligand specificity and evolution of mammalian musk odor receptors: effect of single receptor deletion on odor detection", THE JOURNAL OF NEUROSCIENCE, vol. 36, no. 16, 20 April 2016 (2016-04-20), pages 4482 - 4491, XP009191169
SUZUKI, M. ET AL.: "Deciphering the receptor repertoire encoding specific odorants by time-lapse single- cell array cytometry", SCIENTIFIC REPORTS, vol. 6, 2016, pages 19934, XP055395721, DOI: doi:10.1038/srep19934
GLIA, vol. 16, 1996, pages 247
YOSHIMOTO ET AL., SCIENTIFIC REPORTS, vol. 3, 2013, pages 1191
"GenBank", Database accession no. AY318460
MAINLAND JD ET AL., NATURE NEUROSCIENCE, vol. 17, 2014, pages 114 - 120
SAITO H ET AL., SCIENCE SIGNALING, vol. 2, 2009, pages ra9
IMAMURA MIHO, KAGAKU TO SEIBUTSU, vol. 50, no. 11, 2012
Attorney, Agent or Firm:
SAITO Takuya et al. (JP)
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