Title:
METHOD FOR QUANTIFYING TARGET DNA IN LIVING BODY
Document Type and Number:
WIPO Patent Application WO/2024/014439
Kind Code:
A1
Abstract:
Provided is a novel quantitative method capable of accurately evaluating the dynamics, etc. of a target gene in the body without being affected by the amount of genomic DNA (gDNA). This problem was solved by the following method. A method for quantifying a target DNA that comprises: a step for preparing a liquid sample from a biological material of a measurement target; a step for quantifying the copy number of the target DNA contained in the liquid sample by digital PCR (dPCR); and a step for calculating the copy number of the target DNA per unit amount of the biological material with the use of the copy number of the target DNA in the liquid sample quantified above.
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Inventors:
MATSUMOTO AKIHIRO (JP)
YAMANAKA YOSUKE (JP)
YAMANAKA YOSUKE (JP)
Application Number:
PCT/JP2023/025502
Publication Date:
January 18, 2024
Filing Date:
July 10, 2023
Export Citation:
Assignee:
ASTELLAS PHARMA INC (JP)
International Classes:
C12Q1/68; C12Q1/6806; C12Q1/6851; C12Q1/686
Domestic Patent References:
| WO2021154672A1 | 2021-08-05 |
Other References:
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SUCHY FABIAN P., NISHIMURA TOSHIYA, SEKI SHINSUKE, WILKINSON ADAM C., HIGUCHI MAIMI, HSU IAN, ZHANG JINYU, BHADURY JOYDEEP, NAKAUC: "Streamlined and quantitative detection of chimerism using digital PCR", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 12, no. 1, 17 June 2022 (2022-06-17), US , pages 10223, XP093129051, ISSN: 2045-2322, DOI: 10.1038/s41598-022-14467-5
MIKA THOMAS, MAGHNOUJ ABDELOUAHID, KLEIN-SCORY SUSANNE, LADIGAN-BADURA SWETLANA, BARANISKIN ALEXANDER, THOMSON JULIA, HASENKAMP JU: "Digital-Droplet PCR for Quantification of CD19-Directed CAR T-Cells", FRONTIERS IN MOLECULAR BIOSCIENCES, vol. 7, 15 May 2020 (2020-05-15), XP055893495, DOI: 10.3389/fmolb.2020.00084
DEIANA MICHELA, MORI ANTONIO, PIUBELLI CHIARA, SCARSO SALVATORE, FAVARATO MOSÈ, POMARI ELENA: "Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR", SCIENTIFIC REPORTS, vol. 10, no. 1, 30 October 2020 (2020-10-30), XP093011274, DOI: 10.1038/s41598-020-75958-x
KAIXIANG ZHANG, DONG-KU KANG, M. MONSUR ALI, LINAN LIU, LOUAI LABANIEH, MENGROU LU, HAMIDREZA RIAZIFAR, THI N. NGUYEN, JASON A. ZE: "Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 15, no. 21, 1 January 2015 (2015-01-01), UK , pages 4217 - 4226, XP055646101, ISSN: 1473-0197, DOI: 10.1039/C5LC00650C
ABRAM TIMOTHY J., CHERUKURY HEMANTH, OU CHEN-YIN, VU TAM, TOLEDANO MICHAEL, LI YIYAN, GRUNWALD JONATHAN T., TOOSKY MELODY N., TIFR: "Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 20, no. 3, 4 February 2020 (2020-02-04), UK , pages 477 - 489, XP093129054, ISSN: 1473-0197, DOI: 10.1039/C9LC01212E
PAVŠIČ JERNEJ; ŽEL JANA; MILAVEC MOJCA: "Digital PCR for direct quantification of viruses without DNA extraction", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 408, no. 1, 19 October 2015 (2015-10-19), Berlin/Heidelberg, pages 67 - 75, XP035597463, ISSN: 1618-2642, DOI: 10.1007/s00216-015-9109-0
YANG, HUIYA ET AL.: "184. Developing a rAAV-Based Gene Replacement Therapy for GM3 Synthase Deficiency", MOLECULAR THERAPY, NATURE PUBLISHING GROUP, GB, vol. 28, no. 4, S1, 28 April 2020 (2020-04-28) - 15 May 2020 (2020-05-15), GB , pages 91, XP009551971, ISSN: 1525-0024, DOI: 10.1016/j.ymthe.2020.04.019
SUCHY FABIAN P., NISHIMURA TOSHIYA, SEKI SHINSUKE, WILKINSON ADAM C., HIGUCHI MAIMI, HSU IAN, ZHANG JINYU, BHADURY JOYDEEP, NAKAUC: "Streamlined and quantitative detection of chimerism using digital PCR", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 12, no. 1, 17 June 2022 (2022-06-17), US , pages 10223, XP093129051, ISSN: 2045-2322, DOI: 10.1038/s41598-022-14467-5
MIKA THOMAS, MAGHNOUJ ABDELOUAHID, KLEIN-SCORY SUSANNE, LADIGAN-BADURA SWETLANA, BARANISKIN ALEXANDER, THOMSON JULIA, HASENKAMP JU: "Digital-Droplet PCR for Quantification of CD19-Directed CAR T-Cells", FRONTIERS IN MOLECULAR BIOSCIENCES, vol. 7, 15 May 2020 (2020-05-15), XP055893495, DOI: 10.3389/fmolb.2020.00084
DEIANA MICHELA, MORI ANTONIO, PIUBELLI CHIARA, SCARSO SALVATORE, FAVARATO MOSÈ, POMARI ELENA: "Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR", SCIENTIFIC REPORTS, vol. 10, no. 1, 30 October 2020 (2020-10-30), XP093011274, DOI: 10.1038/s41598-020-75958-x
KAIXIANG ZHANG, DONG-KU KANG, M. MONSUR ALI, LINAN LIU, LOUAI LABANIEH, MENGROU LU, HAMIDREZA RIAZIFAR, THI N. NGUYEN, JASON A. ZE: "Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 15, no. 21, 1 January 2015 (2015-01-01), UK , pages 4217 - 4226, XP055646101, ISSN: 1473-0197, DOI: 10.1039/C5LC00650C
ABRAM TIMOTHY J., CHERUKURY HEMANTH, OU CHEN-YIN, VU TAM, TOLEDANO MICHAEL, LI YIYAN, GRUNWALD JONATHAN T., TOOSKY MELODY N., TIFR: "Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 20, no. 3, 4 February 2020 (2020-02-04), UK , pages 477 - 489, XP093129054, ISSN: 1473-0197, DOI: 10.1039/C9LC01212E
PAVŠIČ JERNEJ; ŽEL JANA; MILAVEC MOJCA: "Digital PCR for direct quantification of viruses without DNA extraction", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 408, no. 1, 19 October 2015 (2015-10-19), Berlin/Heidelberg, pages 67 - 75, XP035597463, ISSN: 1618-2642, DOI: 10.1007/s00216-015-9109-0
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
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