Title:
METHOD FOR QUICKLY EXTRACTING LONG-FRAGMENT GENOMIC DNA BY SINGLE REACTION TUBE, AND KIT
Document Type and Number:
WIPO Patent Application WO/2020/133600
Kind Code:
A1
Abstract:
A method for quickly extracting long-fragment genomic DNA by a single reaction tube, comprising the following steps: a) adding a lysis solution and protease K to a sample, to lyse cells and release genomic DNA; b) adding a precipitant to the reaction system of step a) to obtain a precipitate of genomic DNA; c) washing the obtained precipitate of genomic DNA using a washing solution; and d) dissolving the genomic DNA using a dissolution solution. Also provided is a kit suitable for the method above.
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Inventors:
MAO AIPING (CN)
ZHANG HAIMAN (CN)
ZHANG JIANGUANG (CN)
ZHANG HAIMAN (CN)
ZHANG JIANGUANG (CN)
Application Number:
PCT/CN2019/071678
Publication Date:
July 02, 2020
Filing Date:
January 14, 2019
Export Citation:
Assignee:
BERRY GENOMICS CO LTD (CN)
International Classes:
C12Q1/6806; C12N15/10
Foreign References:
| CN107904229A | 2018-04-13 | |||
| CN103898096A | 2014-07-02 | |||
| CN101413018A | 2009-04-22 | |||
| CN104109663A | 2014-10-22 |
Other References:
CIULLA, T.A. ET AL.: "A Simple Method for DNA Purification from Peripheral Blood.", ANALYTICAL BIOCHEMISTRY, vol. 174, 31 December 1988 (1988-12-31), XP009007530, DOI: 20190521140807X
JACKSON, D.P. ET AL.: "Tissue Extraction of DNA and RNA and Analysis by the Polymerase Chain Reaction.", JOURNAL OF CLINICAL PATHOLOGY, vol. 43, 31 December 1990 (1990-12-31), XP000997196, DOI: 20190521141056X
ROBERTS RJ ET AL.: "The advantages of SMRT sequencing", GENOME BIOL., vol. 14, no. 7, 3 July 2013 (2013-07-03), pages 405, XP055441217
GENOME BIOL., vol. 18, no. 1, 16 August 2017 (2017-08-16), pages 156
JAIN M ET AL.: "Nanopore sequencing and assembly of a human genome with ultra-long reads", NAT BIOTECHNOL., vol. 36, no. 4, April 2018 (2018-04-01), pages 338 - 345
ZHENG GX ET AL.: "Haplotyping germline and cancer genomes with high-throughput linked-read sequencing", NAT BIOTECHNOL., vol. 34, no. 3, March 2016 (2016-03-01), pages 303 - 11, XP055486933, DOI: 10.1038/nbt.3432
ZHANG F ET AL.: "Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube", NAT BIOTECHNOL., vol. 35, no. 9, September 2017 (2017-09-01), pages 852 - 857, XP055584000, DOI: 10.1038/nbt.3897
LAM ET ET AL.: "Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly", NAT BIOTECHNOL., vol. 30, no. 8, August 2012 (2012-08-01), pages 771 - 6
GOLDENBERGER D ET AL.: "A simple ''universal'' DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification", PCR METHODS APPL., vol. 4, no. 6, June 1995 (1995-06-01), pages 368 - 70, XP007923189, DOI: 10.1101/gr.4.6.368
ZHANG Y ET AL.: "A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction", ADV MATER., vol. 28, no. 48, December 2016 (2016-12-01), pages 10630 - 10636, XP055777170, DOI: 10.1002/adma.201603738
MURPHY NM ET AL.: "High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes", METHODS MOL BIOL., vol. 1551, 2017, pages 73 - 85
See also references of EP 3904512A4
JACKSON, D.P. ET AL.: "Tissue Extraction of DNA and RNA and Analysis by the Polymerase Chain Reaction.", JOURNAL OF CLINICAL PATHOLOGY, vol. 43, 31 December 1990 (1990-12-31), XP000997196, DOI: 20190521141056X
ROBERTS RJ ET AL.: "The advantages of SMRT sequencing", GENOME BIOL., vol. 14, no. 7, 3 July 2013 (2013-07-03), pages 405, XP055441217
GENOME BIOL., vol. 18, no. 1, 16 August 2017 (2017-08-16), pages 156
JAIN M ET AL.: "Nanopore sequencing and assembly of a human genome with ultra-long reads", NAT BIOTECHNOL., vol. 36, no. 4, April 2018 (2018-04-01), pages 338 - 345
ZHENG GX ET AL.: "Haplotyping germline and cancer genomes with high-throughput linked-read sequencing", NAT BIOTECHNOL., vol. 34, no. 3, March 2016 (2016-03-01), pages 303 - 11, XP055486933, DOI: 10.1038/nbt.3432
ZHANG F ET AL.: "Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube", NAT BIOTECHNOL., vol. 35, no. 9, September 2017 (2017-09-01), pages 852 - 857, XP055584000, DOI: 10.1038/nbt.3897
LAM ET ET AL.: "Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly", NAT BIOTECHNOL., vol. 30, no. 8, August 2012 (2012-08-01), pages 771 - 6
GOLDENBERGER D ET AL.: "A simple ''universal'' DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification", PCR METHODS APPL., vol. 4, no. 6, June 1995 (1995-06-01), pages 368 - 70, XP007923189, DOI: 10.1101/gr.4.6.368
ZHANG Y ET AL.: "A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction", ADV MATER., vol. 28, no. 48, December 2016 (2016-12-01), pages 10630 - 10636, XP055777170, DOI: 10.1002/adma.201603738
MURPHY NM ET AL.: "High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes", METHODS MOL BIOL., vol. 1551, 2017, pages 73 - 85
See also references of EP 3904512A4
Attorney, Agent or Firm:
KING & WOOD MALLESONS (CN)
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